Team:Hong Kong HKUST/notebook/mod3

From 2013.igem.org

(Difference between revisions)
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<div>
<div>
       <a href='#' class='head'>Week 1</a>
       <a href='#' class='head'>Week 1</a>
-
       <div class='content' align="left">Content 1
+
       <div class='content' align="left">
 +
<p>&nbsp;</p>
 +
<p><strong>August 5</strong></p>
 +
<p>GFP (25)PCR Check with VR VF2</p>
 +
<ul>
 +
  <blockquote>
 +
    <p>- Gel check(overan)</p>
 +
  </blockquote>
 +
</ul>
 +
<p>BBa_J176171</p>
 +
<ul>
 +
  <blockquote>
 +
    <p>- Digestion using SpeI and PstI-HF to extract the backbone</p>
 +
  </blockquote>
 +
</ul>
 +
<p>PolyA / BBa_K404108</p>
 +
<ul>
 +
  <blockquote>
 +
    <p>- Digestion using XbaI and PstI-HF</p>
 +
    <p>- Gel electrophoresis</p>
 +
    <p>- Run Gel </p>
 +
  </blockquote>
 +
</ul>
 +
<p>Inoculation of pSB1C3</p>
 +
<p>&nbsp;</p>
 +
<p><strong>August 6</strong></p>
 +
<p>GFP (25)</p>
 +
<ul>
 +
  <blockquote>
 +
    <p>- Repeat PCR check</p>
 +
    <p>- Run Gel</p>
 +
  </blockquote>
 +
</ul>
 +
<p>pSB1C3</p>
 +
<ul>
 +
  <blockquote>
 +
    <p>- Plasmid extraction</p>
 +
  </blockquote>
 +
</ul>
 +
<p>PolyA / BBa_K404108</p>
 +
<ul>
 +
  <blockquote>
 +
    <p>- Gel purification</p>
 +
    <p>- Gel check</p>
 +
  </blockquote>
 +
</ul>
 +
<p>BBa_J176171</p>
 +
<ul>
 +
  <blockquote>
 +
    <p>- PCR clean up</p>
 +
  </blockquote>
 +
</ul>
 +
<p>BBa_K929000 &amp; BBa_J52034 </p>
 +
<ul>
 +
  <blockquote>
 +
    <p>- Transformation using CAM plate</p>
 +
  </blockquote>
 +
</ul>
 +
<p>MLS</p>
 +
<ul>
 +
  <blockquote>
 +
    <p>- PCR amplify MLS in RFC 10 and 25 format</p>
 +
  </blockquote>
 +
</ul>
 +
<p>&nbsp;</p>
 +
<p><strong>August 7</strong></p>
 +
<p>MLS (RFC 10 &amp; 25)</p>
 +
<ul>
 +
  <blockquote>
 +
    <p>- PCR clean up</p>
 +
    <p>- Nanodrop for the PCR clean up product</p>
 +
    <p>- Gel check for the PCR clean up product</p>
 +
    <p>- Digestion using EcoRI and PstI</p>
 +
  </blockquote>
 +
</ul>
 +
<p>PolyA / BBa_K404108</p>
 +
<ul>
 +
  <blockquote>
 +
    <p>- Gel check</p>
 +
  </blockquote>
 +
</ul>
 +
<p>pSB1C3 </p>
 +
<ul>
 +
  <blockquote>
 +
    <p>- Digest with EcoRI &amp; PstI </p>
 +
    <p>- Run gel and cut gel </p>
 +
  </blockquote>
 +
</ul>
 +
<p>GFP(25) </p>
 +
<ul>
 +
  <blockquote>
 +
    <p>- Digestion with EcoRI &amp; SpeI</p>
 +
    <p>- Run gel and cut gel </p>
 +
  </blockquote>
 +
</ul>
 +
<p>&nbsp;</p>
 +
<p><strong>August 8</strong></p>
 +
<p>GFP25 &amp; pSB1C3</p>
 +
<ul>
 +
<blockquote>
 +
  <p>- Gel purification</p>
 +
    <p>- Nanodrop</p>
 +
    <p>- Gel check </p>
 +
  </blockquote>
 +
</ul>
 +
<p>polyA</p>
 +
<ul>
 +
  <blockquote>
 +
    <p>- Digestion with EcoRI and XbaI</p>
 +
    <p>- PCR clean up</p>
 +
  </blockquote>
 +
</ul>
 +
<p>BBa_K929000 &amp; BBa_J52034 </p>
 +
<ul>
 +
  <blockquote>
 +
    <p>- Inoculation </p>
 +
  </blockquote>
 +
</ul>
 +
<p>&nbsp;</p>
 +
<p><strong>August 9</strong></p>
 +
<p>BBa_K929000 &amp; BBa_J52034 </p>
 +
<p>- Plasmid extraction: J52034 is not transformed</p>
 +
<p>MLS.pSB1C3 &amp; GFP.PolyA</p>
 +
<p>- Ligation</p>
 +
<p>- Transformation to SURE on CAM plate</p>
 +
<p>&nbsp;</p>
 +
 
       </div>
       </div>
     </div>
     </div>

Revision as of 17:01, 19 September 2013








June 2013

Week 4
Wet lab
· Inoculation of pBlueScript KS(+) for training
· Autoclave basic materials
· Preparing LB
· LB-Ampicillin plates poured
Dry lab
Protocols review

July 2013

Week 1
Content 1
Week 2
Content 1
Week 3
Content 1
Week 4
Content 1
Week 5
Content 1

August 2013

Week 1

 

August 5

GFP (25)PCR Check with VR VF2

    - Gel check(overan)

BBa_J176171

    - Digestion using SpeI and PstI-HF to extract the backbone

PolyA / BBa_K404108

    - Digestion using XbaI and PstI-HF

    - Gel electrophoresis

    - Run Gel

Inoculation of pSB1C3

 

August 6

GFP (25)

    - Repeat PCR check

    - Run Gel

pSB1C3

    - Plasmid extraction

PolyA / BBa_K404108

    - Gel purification

    - Gel check

BBa_J176171

    - PCR clean up

BBa_K929000 & BBa_J52034

    - Transformation using CAM plate

MLS

    - PCR amplify MLS in RFC 10 and 25 format

 

August 7

MLS (RFC 10 & 25)

    - PCR clean up

    - Nanodrop for the PCR clean up product

    - Gel check for the PCR clean up product

    - Digestion using EcoRI and PstI

PolyA / BBa_K404108

    - Gel check

pSB1C3

    - Digest with EcoRI & PstI

    - Run gel and cut gel

GFP(25)

    - Digestion with EcoRI & SpeI

    - Run gel and cut gel

 

August 8

GFP25 & pSB1C3

    - Gel purification

    - Nanodrop

    - Gel check

polyA

    - Digestion with EcoRI and XbaI

    - PCR clean up

BBa_K929000 & BBa_J52034

    - Inoculation

 

August 9

BBa_K929000 & BBa_J52034

- Plasmid extraction: J52034 is not transformed

MLS.pSB1C3 & GFP.PolyA

- Ligation

- Transformation to SURE on CAM plate

 

Week 2
Content 1
Week 3
Content 1
Week 4
Content 1

September 2013

Week 1
Content 1
Week 2
Content 1
Week 3
Content 1
Week 4
Content 1
Week 5
Content 1

Retrieved from "http://2013.igem.org/Team:Hong_Kong_HKUST/notebook/mod3"