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From 2013.igem.org

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-	<h1 id='Background'>Background</h1>	+	<h1 id='Background'>Background</h1>The optimization of bacterial compound expression has its roots in metabolic pathways. Often, the production pathways of multiple precursor compounds require tuning before a synthetic system can work at full efficiency. As a result, the use of different light wavelengths to manipulate expression levels was of interest to the 2012 iGEM Team. Light expression is cheap relative to chemical methods. Moreover, light expression systems can undergo fine manipulation through changes in intensity, and can undergo rapid removal of inputs in a simple, straightforward manner. Finally, many light expression systems are reversible, depending on the wavelength of light used. The 2012 iGEM Team attempted to modulate expression of beta-galactosidase using a green light sensor and red light sensor. The final results were unsatisfactory; therefore, the 2013 iGEM Team sought to improve and refine compound expression through the use of the green and red light sensors.
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Revision as of 21:12, 19 September 2013

Contents 1 Background 1.1 Cloning 2 [Top] 2.1 Our System 3 [Top]

Background

The optimization of bacterial compound expression has its roots in metabolic pathways. Often, the production pathways of multiple precursor compounds require tuning before a synthetic system can work at full efficiency. As a result, the use of different light wavelengths to manipulate expression levels was of interest to the 2012 iGEM Team. Light expression is cheap relative to chemical methods. Moreover, light expression systems can undergo fine manipulation through changes in intensity, and can undergo rapid removal of inputs in a simple, straightforward manner. Finally, many light expression systems are reversible, depending on the wavelength of light used. The 2012 iGEM Team attempted to modulate expression of beta-galactosidase using a green light sensor and red light sensor. The final results were unsatisfactory; therefore, the 2013 iGEM Team sought to improve and refine compound expression through the use of the green and red light sensors.

Cloning

[Top]

CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

ccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

Our System

[Top]

SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS SSSSSSSSSSSSSSSSSSSSSSSSSSS sssssssssssssssssssssssssss SSSSSSSSSSSSSSSSSSSSSSSSS