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Team:Wisconsin-Madison/teammembers
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<h1>UW Madison iGEM Team Members</h1> | <h1>UW Madison iGEM Team Members</h1> | ||
<h2> <strong>Matt Schmitz</strong></h2> | <h2> <strong>Matt Schmitz</strong></h2> | ||
| - | <p align="left" class = "classtheinlinecontent2">Matt Schmitz is a thermostable DNA ligase, | + | <p align="left" class = "classtheinlinecontent2">Matt Schmitz is a thermostable DNA ligase, who joins adjacent 5' and 3' ends of DNA, removing nicks in double stranded DNA. He is active from 45-65 degrees Celsius(Barany, F. (1991). Proc. Natl. Acad. Sci. USA. 88, 189-193). Matt Schmitz's gene is within a kanamycin resistant PET-ALM1 plasmid, downstream of the T7 promoter, upstream of the T7 terminator. Thus, Matt Schmitz can be used with an inducible T7 expression system to express himself. Matt has an N-Terminus hexa-histidine tag, joined to the wild type him by a thrombin cleavage motif. </p> |
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Revision as of 04:43, 20 September 2013
UW Madison iGEM Team Members
Matt Schmitz
Matt Schmitz is a thermostable DNA ligase, who joins adjacent 5' and 3' ends of DNA, removing nicks in double stranded DNA. He is active from 45-65 degrees Celsius(Barany, F. (1991). Proc. Natl. Acad. Sci. USA. 88, 189-193). Matt Schmitz's gene is within a kanamycin resistant PET-ALM1 plasmid, downstream of the T7 promoter, upstream of the T7 terminator. Thus, Matt Schmitz can be used with an inducible T7 expression system to express himself. Matt has an N-Terminus hexa-histidine tag, joined to the wild type him by a thrombin cleavage motif.
