"
Team:Wisconsin-Madison/teammembers
From 2013.igem.org
(Difference between revisions)
Cbjohnson4 (Talk | contribs) |
Cbjohnson4 (Talk | contribs) |
||
| Line 26: | Line 26: | ||
<h2> <strong>Matt Schmitz</strong></h2> | <h2> <strong>Matt Schmitz</strong></h2> | ||
| - | <img src="https://mywebspace.wisc.edu/mtschmitz/website%20files/matt.jpg" height="300" width=" | + | <img src="https://mywebspace.wisc.edu/mtschmitz/website%20files/matt.jpg" height="300" width="270"> |
<p align="left" class = "classtheinlinecontent2">Matt Schmitz is a thermostable DNA ligase, who joins adjacent 5' and 3' ends of DNA, removing nicks in double stranded DNA. He is active from 45-65 degrees Celsius(Barany, F. (1991). Proc. Natl. Acad. Sci. USA. 88, 189-193). Matt Schmitz's gene is within a kanamycin resistant PET-ALM1 plasmid, downstream of the T7 promoter, upstream of the T7 terminator. Thus, Matt Schmitz can be used with an inducible T7 expression system to express himself. Matt has an N-Terminus hexa-histidine tag, joined to the wild him by a thrombin cleavage motif. </p> | <p align="left" class = "classtheinlinecontent2">Matt Schmitz is a thermostable DNA ligase, who joins adjacent 5' and 3' ends of DNA, removing nicks in double stranded DNA. He is active from 45-65 degrees Celsius(Barany, F. (1991). Proc. Natl. Acad. Sci. USA. 88, 189-193). Matt Schmitz's gene is within a kanamycin resistant PET-ALM1 plasmid, downstream of the T7 promoter, upstream of the T7 terminator. Thus, Matt Schmitz can be used with an inducible T7 expression system to express himself. Matt has an N-Terminus hexa-histidine tag, joined to the wild him by a thrombin cleavage motif. </p> | ||
Revision as of 05:00, 20 September 2013
UW Madison iGEM Team Members
Haley Bomb
Boom.
Matt Schmitz
Matt Schmitz is a thermostable DNA ligase, who joins adjacent 5' and 3' ends of DNA, removing nicks in double stranded DNA. He is active from 45-65 degrees Celsius(Barany, F. (1991). Proc. Natl. Acad. Sci. USA. 88, 189-193). Matt Schmitz's gene is within a kanamycin resistant PET-ALM1 plasmid, downstream of the T7 promoter, upstream of the T7 terminator. Thus, Matt Schmitz can be used with an inducible T7 expression system to express himself. Matt has an N-Terminus hexa-histidine tag, joined to the wild him by a thrombin cleavage motif.
Brit Zaro
Kevin Jiang
