Team:Virginia/Notebook

From 2013.igem.org

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!align="center"|[[Team:Virginia|Home]]
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!align="center"|[[Team:Virginia/Team|Team]]
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<head>
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Virginia Official Team Profile]
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<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
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!align="center"|[[Team:Virginia/Project|Project]]
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<title>VGEM Welcomes You!</title>
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!align="center"|[[Team:Virginia/Human_Practices|Human Practices]]
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!align="center"|[[Team:Virginia/Parts|Parts Submitted to the Registry]]
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<style type="text/css">
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!align="center"|[[Team:Virginia/Modeling|Modeling]]
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!align="center"|[[Team:Virginia/Notebook|Notebook]]
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!align="center"|[[Team:Virginia/Safety|Safety]]
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!align="center"|[[Team:Virginia/Attributions|Attributions]]
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</head>
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May 20:
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<body>
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• Organized Gilmer 147 lab space
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<div class="igemlogo">
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• Tour of Gilmer lab facilities from Dr. Kay Christopher
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<a href="https://2013.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/igem.org/1/17/IGEM_basic_Logo_white_stylized.png" width="70"> </a>
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• Discussed work schedules. Typically, there will be people in the lab from 9am to 9pm, working staggered 8-hour shifts.
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</div>
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• Finance: Received $7500 from Medical School, expecting $25,000 each from College and Engineering (total: $65,000 raised, $9000 left over from last year)
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<div class="topbanner">
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• Reviewed and discussed project proposals.  
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  <div id="logo">
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• List of buffers we need:
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<p id="VGEM"><a href="https://2013.igem.org/Team:Virginia"><span>V</span>GEM</a></p>
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<p><a href="http://www.virginia.edu/"> University of Virginia</a></p>
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May 21:
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</div>
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• Discussed modified CRISPR idea. This is different from the original project proposal. We would abandon the Cmr-crRNA complexes and use the MS2 coat proteins to export shRNA sequences via use of a CRISPR array. This array consists of a promoter with Cas6 (cleaves repeats in the array) and shRNA sequences that are complimentary to knockdown targets. The double stranded RNA is then cleaved by a eukaryotic cell’s natural Dicer system.
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  <div id="menu11">
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• If we wanted to use a human cell line (BSL-2), we would have to write it into our PI’s (Kozminski’s) protocol and get approval from Environmental Health and Safety. Dr. Christopher says that HeLa cells are easy to grow, but generally difficult to transfect. However, mouse cell lines are BSL-1 and are easily transfected and there is a lot of literature available to see if particular lines can express whatever surface receptors (ß-1, etc.). We will need to talk to Kozminski to obtain BSL-2 approval, however, since transfecting E. coli with invasin will allow them to be phagocytosed by eukaryotic cells.
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    <ul>
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• Kozminski says we will need to determine BSL’s and clearly outline our protocols before contacting EHS. We should do this via him or Dr. Christopher.
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        <li>
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• Description of biosafetly levels: http://www.cdc.gov/biosafety/publications/bmbl5/BMBL5_sect_IV.pdf
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                <span class="title">Home</span>
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• Shaun, Josh, Matt, Greg, Chris L, Jon, Bethany and Liz completed BSL-1 training
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                <span class="text"><a href="https://2013.igem.org/Team:Virginia">The start</a></span>
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        </li>
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List of to Do’s
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        <li>
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- PTAO number
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        <span class="title">Project</span>
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                <span class="text">
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                <p><a href="https://2013.igem.org/Team:Virginia/Project_Overview">Overview</a></p>
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                <p><a href="https://2013.igem.org/Team:Virginia/Background">Background</a></p>
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                <p><a href="https://2013.igem.org/Team:Virginia/Parts">Parts</a></p>
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                <p><a href="https://2013.igem.org/Team:Virginia/Results">Results</a></p>
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                <p><a href="https://2013.igem.org/Team:Virginia/Modeling">Modeling</a></p>
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                <p><a href="https://2013.igem.org/Team:Virginia/Applications">Applications</a></p></span>
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        </li>
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        <li>
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                <span class="title">Notebook</span>
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                <span class="text">
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                <p><a href="https://2013.igem.org/Team:Virginia/Notebook_Overview">Overview</a></p>
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                <p><a href="https://2013.igem.org/Team:Virginia/Notebook">Notebook</a></p>
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                <p><a href="https://2013.igem.org/Team:Virginia/Protocols">Protocols</a></p>
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                <p><a href="https://2013.igem.org/Team:Virginia/Gallery">Gallery</a></p></span>
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        </li>
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        <li>
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                <span class="title">Human Practices</span>
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                <span class="text">
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                <p><a href="https://2013.igem.org/Team:Virginia/Human_Practices_Overview">Overview</a></p>
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                <p><a href="https://2013.igem.org/Team:Virginia/Public_Perception">Public Perception</a></p>
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                <p><a href="https://2013.igem.org/Team:Virginia/Relevance">Relevance</a></p>
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          <p><a href="https://2013.igem.org/Team:Virginia/Outreach">Outreach</a></p></span>
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        </li>
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        <li>
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                <span class="title">Team</span>
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  <span class="text">
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                <p><a href="https://2013.igem.org/Team:Virginia/Team_Overview">Overview</a></p>
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                <p><a href="https://2013.igem.org/Team:Virginia/Bios">Bios</a></p>
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                <p><a href="https://igem.org/Team.cgi?year=2013&team_name=Virginia">Profile</a></p>
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                <p><a href="https://2013.igem.org/Team:Virginia/Sponsors">Sponsors</a></p>
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      <p><a href="https://2013.igem.org/Team:Virginia/Collaboration">Collaboration</a></p></span></li></ul></div></div>
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<div class="body">
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<div class="footer">
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  <h3>&nbsp;</h3>
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<h3>Contact</h3>
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<p>Questions? Comments? Email us at <a href="virginia.igem@gmail.com">virginia.igem@gmail.com</a></p>
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</div>
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<p>&nbsp;</p>
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</body>
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</html>

Revision as of 20:35, 21 September 2013

VGEM Welcomes You!

 

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