Template:Kyoto/Notebook/Aug 30

From 2013.igem.org

(Difference between revisions)
(→Aug 30)
(→Aug 30)
Line 7: Line 7:
!Name||quantity||concentration[µg/mL]||260/280||260/230
!Name||quantity||concentration[µg/mL]||260/280||260/230
|-
|-
-
|PT181 atte(2) Ets||305.3 ||2.2 ||1.66 ||0.36
+
|PT181 atte(2) E+S||305.3 ||2.2 ||1.66 ||0.36
|-
|-
-
|PT181 atte(2) Xtp||217.7 ||3.9 ||1.73 ||0.04
+
|PT181 atte(2) X+P||217.7 ||3.9 ||1.73 ||0.04
|-
|-
-
|PT181 anti(2) Ets||347.7 ||3.1 ||1.88 ||0.31
+
|PT181 anti(2) E+S||347.7 ||3.1 ||1.88 ||0.31
|-
|-
-
|PT181 anti(2) Xtp||376.5 ||5.9 ||0.49 ||2.36
+
|PT181 anti(2) X+P||376.5 ||5.9 ||0.49 ||2.36
|-
|-
-
|PSBIC3 Ets||436.6 ||30.7 ||1.03 ||1.28
+
|PSBIC3 E+S||436.6 ||30.7 ||1.03 ||1.28
|-
|-
-
|PSBIC3 Xtp||457.4 ||7.9 ||1.65 ||0.42
+
|PSBIC3 X+P||457.4 ||7.9 ||1.65 ||0.42
|-
|-
-
|Spinach Ets||490.5 ||3.0 ||1.87 ||0.26
+
|Spinach E+S||490.5 ||3.0 ||1.87 ||0.26
|-
|-
-
|tetR aptamer 12-1R Ets||451.7 ||-12.8 ||5.56 ||-0.04
+
|tetR aptamer 12-1R E+S||451.7 ||-12.8 ||5.56 ||-0.04
|}
|}
</div>
</div>
Line 69: Line 69:
!DNA||concentration[&micro;g/mL]||260/280||260/230  
!DNA||concentration[&micro;g/mL]||260/280||260/230  
|-
|-
-
|pT181 atte-(2) Et3 || || ||
+
|pT181 atte-(2) E+S || || ||
|-
|-
-
|pT181 atte-(2) Xtp || || ||
+
|pT181 atte-(2) X+P || || ||
|-
|-
-
|pT181 anti-(2) Et3 || || ||
+
|pT181 anti-(2) E+S || || ||
|-
|-
-
|pT181 anti-(2) Xtp || || ||
+
|pT181 anti-(2) X+P || || ||
|-
|-
-
|pSB1C3 Et3 || || ||
+
|pSB1C3 E+S || || ||
|-
|-
-
|pSB1C3 Xtp || || ||
+
|pSB1C3 X+P || || ||
|-
|-
-
|Spinach Et3 || || ||
+
|Spinach E+S || || ||
|-
|-
-
|tetR aptamer 12_1R Et3 || || ||
+
|tetR aptamer 12_1R E+S || || ||
 +
|}
 +
</div>
 +
<!-- ここまでをコピーしてね -->
 +
===Restriction Enzyme Digestion===
 +
<!-- こっから -->
 +
<div class="experiment">
 +
<span class="author">じっけんしゃ</span>
 +
{| class="wikitable"
 +
! ||RBS-lysis2 (9)||EcoRI||SpeI||10x bufferB||100x BSA||MilliQ||total
 +
|-
 +
|2 cuts||7.5 &micro;L||0.5 &micro;L||0.5 &micro;L||3 &micro;L||0.3 &micro;L||18.2 &micro;L||30 &micro;L
 +
|-
 +
|NC||0.5 &micro;L||0 &micro;L||0 &micro;L||1 &micro;L||0.1 &micro;L||8.4 &micro;L||10 &micro;L
|}
|}
</div>
</div>
<!-- ここまでをコピーしてね -->
<!-- ここまでをコピーしてね -->

Revision as of 08:46, 22 September 2013

Contents

Aug 30

Gel Extraction

No Name

Namequantityconcentration[µg/mL]260/280260/230
PT181 atte(2) E+S305.3 2.2 1.66 0.36
PT181 atte(2) X+P217.7 3.9 1.73 0.04
PT181 anti(2) E+S347.7 3.1 1.88 0.31
PT181 anti(2) X+P376.5 5.9 0.49 2.36
PSBIC3 E+S436.6 30.7 1.03 1.28
PSBIC3 X+P457.4 7.9 1.65 0.42
Spinach E+S490.5 3.0 1.87 0.26
tetR aptamer 12-1R E+S451.7 -12.8 5.56 -0.04

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
RBS-lysis2 9264.6　1.97　2.21
RBS-lysis2 12220.0　1.89　2.46
RBS-lysis2 14195.3　1.88　1.53

Colony PCR

No name

Samplebase pair
8/29 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT(8/20 LB Amp) (1)~(4)2143
8/29 Plux-RBS-GFP-DT(8/27 LB-CP) (1)~(2)1227
8/29 Pbad-larac-RBS-RFP{BBaI13516} (8/21 LB-CP) (1)~(2)2256
8/29 RBS-lysis3-DT-(1)1210
PreDenatureDenatureAnnealingExtensioncycle
　94°C　94°C　55°C　68°C--
5min　30s　30s　2min　30cycle

Ethanol Precipitation

No name

DNAconcentration[µg/mL]260/280260/230
pT181 atte-(2) E+S
pT181 atte-(2) X+P
pT181 anti-(2) E+S
pT181 anti-(2) X+P
pSB1C3 E+S
pSB1C3 X+P
Spinach E+S
tetR aptamer 12_1R E+S

Restriction Enzyme Digestion

じっけんしゃ

RBS-lysis2 (9)EcoRISpeI10x bufferB100x BSAMilliQtotal
2 cuts7.5 µL0.5 µL0.5 µL3 µL0.3 µL18.2 µL30 µL
NC0.5 µL0 µL0 µL1 µL0.1 µL8.4 µL10 µL
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