Template:Kyoto/Notebook/Aug 30

(Difference between revisions)

Revision as of 11:16, 22 September 2013

No Name

Name	quantity	concentration[µg/mL]	260/280	260/230
pT181 attenuator(2) (EcoRI & SpeI)	305.3	2.2	1.66	0.36
pT181 attenuator(2) (XbaI & PstI)	217.7	3.9	1.73	0.04
pT181 antisense(2) (EcoRI & SpeI)	347.7	3.1	1.88	0.31
pT181 antisense(2) (XbaI & PstI)	376.5	5.9	0.49	2.36
pSB1C3 (EcoRI & SpeI)	436.6	30.7	1.03	1.28
pSB1C3 (XbaI & PstI)	457.4	7.9	1.65	0.42
Spinach (EcoRI & SpeI)	490.5	3.0	1.87	0.26
tetR aptamer 12_1R (EcoRI & SpeI)	451.7	-12.8	5.56	-0.04

No name

DNA	concentration[µg/mL]	260/280	260/230
RBS-lysis2 9	264.6	1.97	2.21
RBS-lysis2 12	220.0	1.89	2.46
RBS-lysis2 14	195.3	1.88	1.53

No name

Sample	base pair
8/29 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT(8/20 LB Amp) (1)~(4)	2143
8/29 Plux-RBS-GFP-DT(8/27 LB-CP) (1)~(2)	1227
8/29 Pbad/araC-RBS-RFP{BBa_I13516} (8/21 LB-CP) (1)~(2)	2256
8/29 RBS-lysis3-DT-(1)	1210

PreDenature	Denature	Annealing	Extension	cycle
94°C	94°C	55°C	68°C	--
5min	30s	30s	2min	30cycle

No name

No name

	8/30 RBS-lysis2 (9)	EcoRI	SpeI	10x bufferB	100x BSA	MilliQ	total
2 cuts	7.5 µL	0.5 µL	0.5 µL	3 µL	0.3 µL	18.2 µL	30 µL
NC	0.5 µL	0 µL	0 µL	1 µL	0.1 µL	8.4 µL	10 µL

	8/22 pSB1C3-(2)	EcoRI	SpeI	10x Buffer	100x BSA	MilliQ	total
2 cuts	8 µL	0.5 µL	0.5 µL	3 µL	0.3 µL	17.7 µL	30 µL
NC	1 µL	0 µL	0 µL	1 µL	0.1 µL	7.9 µL	10 µL

	8/22 pSB1C3-(2)	XbaI	PstI	BufferD	BSA	MilliQ	total
2 cuts	8 µL	0.5 µL	0.5 µL	3 µL	0.3 µL	17.7 µL	30 µL
NC	1 µL	0 µL	0 µL	1 µL	0.1 µL	7.9 µL	10 µL

	8/21 Spinach (1)	EcoRI	SpeI	10x BufferB	100x BSA	MilliQ	total
2 cuts	4 µL	0.5 µL	0.5 µL	3 µL	0.3 µL	21.9 µL	30 µL
NC	0.5 µL	0 µL	0 µL	1 µL	0.1 µL	8.4 µL	10 µL

	8/21 tetR aptamer 12_1R (1)	EcoRI	SpeI	10xBufferB	100xBSA	MilliQ	total
2 cuts	3 µL	0.5 µL	0.5 µL	3 µL	0.3 µL	22.7 µL	30 µL
NC	0.4 µL	0 µL	0 µL	1 µL	0.1 µL	8.4 µL	10 µL

	8/21 pT181 attenuator-(2)	EcoRI	SpeI	XbaI	PstI	10x BufferB	10x BufferD	100x BSA	MilliQ	total
2 cuts	4 µL	0.5 µL	0.5 µL	0 µL	0 µL	3 µL	0 µL	0.3 µL	21.7 µL	30 µL
NC	0.5 µL	0 µL	0 µL	0 µL	0 µL	1 µL	0 µL	0.1 µL	8.4 µL	10 µL
2 cuts	4 µL	0 µL	0 µL	0.5 µL	0.5 µL	0 µL	3 µL	0.3 µL	21.7 µL	30 µL
NC	0.5 µL	0 µL	0 µL	0 µL	0 µL	0 µL	1 µL	0.1 µL	8.4 µL	10 µL

	8/21 pT181 antisense-(2)	EcoRI	SpeI	XbaI	PstI	10x BufferB	10x BufferD	100x BSA	MilliQ	total
2 cuts	9 µL	0.5 µL	0.5 µL	0 µL	0 µL	3 µL	0 µL	0.3 µL	16.7 µL	30 µL
NC	1 µL	0 µL	0 µL	0 µL	0 µL	1 µL	0 µL	0.1 µL	7.9 µL	10 µL
2 cuts	9 µL	0 µL	0 µL	0.5 µL	0.5 µL	0 µL	3 µL	0.3 µL	16.7 µL	30 µL
NC	1 µL	0 µL	0 µL	0 µL	0 µL	0 µL	1 µL	0.1 µL	7.9 µL	10 µL

No name

Lane	Sample	Enzyme1	Enzyme2
1	100bp ladder	--	--
2	RBS-lysis2-(9)	EcoRI	SpeI
3	RBS-lysis2-(9)	--	--
4	pSB1C3-(2)	EcoRI	SpeI
5	pSB1C3-(2)	--	--
6	pSB1C3-(2)	XbaI	PstI
7	pSB1C3-(2)	--	--
8	tRNA-Spinach-(1)	EcoRI	SpeI
9	tRNA-Spinach-(1)	--	--
10	tetR aptamer12_1R	EcoRI	SpeI
11	tetR aptamer12_1R	--	--
12	pT181 attenuator	EcoRI	SpeI
13	pT181 attenuator	EcoRI	SpeI
14	100bp ladder	--	--

No name

Lane	Sample	Enzyme1	Enzyme2
1	8/22 RBS-lysis1-(1)	EcoRI	SpeI
2	8/22 RBS-lysis1-(1)	--	--
3	8/17 RBS-GFP-DT-(2)	XbaI	PstI
4	8/17 RBS-GFP-DT-(2)	--	--
5	8/20 Pcon-RBS-GFP-DT-(1)	EcoRI	XbaI
6	8/20 Pcon-RBS-GFP-DT-(1)	--	--
7	100bp ladder	--	--

@@ Line 151: / Line 151: @@
 !Lane||Sample||Enzyme1||Enzyme2
 |-
-|1||RBS-lysis2-(9)||EcoRI||SpeI
+|1||100bp ladder||--||--
 |-
-|2||RBS-lysis2-(9)||--||--
+|2||RBS-lysis2-(9)||EcoRI||SpeI
 |-
-|3||pSB1C3-(2)||EcoRI||SpeI
+|3||RBS-lysis2-(9)||--||--
 |-
-|4||pSB1C3-(2)||--||--
+|4||pSB1C3-(2)||EcoRI||SpeI
 |-
-|5||pSB1C3-(2)||XbaI||PstI
+|5||pSB1C3-(2)||--||--
 |-
-|6||pSB1C3-(2)||--||--
+|6||pSB1C3-(2)||XbaI||PstI
 |-
-|7||tRNA-Spinach-(1)||EcoRI||SpeI
+|7||pSB1C3-(2)||--||--
 |-
-|8||tRNA-Spinach-(1)||--||--
+|8||tRNA-Spinach-(1)||EcoRI||SpeI
 |-
-|9||tetR aptamer12_1R||EcoRI||SpeI
+|9||tRNA-Spinach-(1)||--||--
 |-
-|10||tetR aptamer12_1R||--||--
+|10||tetR aptamer12_1R||EcoRI||SpeI
 |-
-|11||pT181 attenuator||EcoRI||SpeI
+|11||tetR aptamer12_1R||--||--
 |-
 |12||pT181 attenuator||EcoRI||SpeI
+|-
+|13||pT181 attenuator||EcoRI||SpeI
+|-
+|14||100bp ladder||--||--
+|}
+[[File:igku_Aug30electrophoresis1]]<br>
+</div>
+===Electrophoresis===
+<div class="experiment">
+<span class="author">No name</span>
+{| class="wikitable"
+!Lane||Sample||Enzyme1||Enzyme2
+|-
+|1||8/22 RBS-lysis1-(1)||EcoRI||SpeI
+|-
+|2||8/22 RBS-lysis1-(1)||--||--
+|-
+|3||8/17 RBS-GFP-DT-(2)||XbaI||PstI
+|-
+|4||8/17 RBS-GFP-DT-(2)||--||--
+|-
+|5||8/20 Pcon-RBS-GFP-DT-(1)||EcoRI||XbaI
+|-
+|6||8/20 Pcon-RBS-GFP-DT-(1)||--||--
+|-
+|7||100bp ladder||--||--
 |}
-[[File:igku_Aug28electrophoresis1]]<br>
+[[File:igku_Aug30electrophoresis2]]<br>
 </div>