Template:Kyoto/Notebook/Aug 31

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Revision as of 13:00, 22 September 2013

Aug 31

Gel Extraction

No name

LaneDNAEnzyme
1100bp ladder--
2----
3RBS-lysis2-(9)EcoRI & SpeI
4
5----
6pSB1C3(1)EcoRI & SpeI
7
8----
9pSB1C3(1)XbaI&PstI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

LaneDNAEnzyme
1100bp ladder--
2tRNA-SpinachEcoRI & SpeI
3
4----
5pT181 attenuator-(2)EcoRI & SpeI
6
7pT181 attenuator-(2)XbaI & PstI
8
9----
10pT181 antisense-(2)EcoRI & SpeI
11

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

LaneDNAEnzyme
1100bp ladder--
2pT181 antisenseXbaRI & PstI
3
4----
5tetR aptamer 12_1R-(1)EcoRI & SpeI
6
Nameconcentration[µg/mL]260/280260/230
Pcon-RBS-GFP-DT (EcoRI&SpeI)7.11.880.07
Pcon-RBS-luxR-DT (EcoRI&XbaI)15.31.930.70
Plux (SpeI&PstI)4.51.790.31
RBS-GFP-DT (XbaI&PstI)6.02.160.03
RBS-tetR-DT (XbaI&PstI)7.61.910.38