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Template:Kyoto/Notebook/Aug 31
From 2013.igem.org
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|tetR aptamer 12_1R(EcoRI&SpeI)||--||--||-- | |tetR aptamer 12_1R(EcoRI&SpeI)||--||--||-- | ||
| + | |} | ||
| + | ===Ligation=== | ||
| + | <div class="experiment"> | ||
| + | <span class="author">Nakamoto and Hirano</span> | ||
| + | {| class="wikitable" | ||
| + | !state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2 | ||
| + | |- | ||
| + | |experiment||8/30 pSB1C3(EcoRI&SpeI)||6.6||8/30 tRNA-Spinach(EcoRI&SpeI)||12.5||3.5 | ||
| + | |- | ||
| + | |experiment||8/30 pSB1C3(EcoRI&SpeI)||6.6||8/30 pT181 antisense(EcoRI&SpeI)||12.5||3.5 | ||
| + | |- | ||
| + | |experiment||8/30 pSB1C3(XbaI&Pst)||12.5||8/30 pT181 attenuator(XbaI&PstI)||25||3.5 | ||
| + | |- | ||
| + | |experiment||8/30 pSB1C3(XbaI&Pst)||12.5||8/30 pT181 antisense(XbaI&PstI)||25||3.5 | ||
| + | |- | ||
| + | |experiment||8/20 DT||6.6||8/30 pT181 attenuator(2)||12.5||3.5 | ||
| + | |- | ||
| + | |experiment||8/20 DT||6.6||8/30 pT181 antisense||12.5||3.5 | ||
| + | |- | ||
| + | |experiment||8/27 DT||3.0||8/30 tRNA-Spinach(EcoRI&SpeI)||12.5||3.5 | ||
| + | |- | ||
| + | |experiment||8/20 DT||6.6||8/30 RBS-lysis2||12.5||3.5 | ||
| + | |- | ||
| + | |experiment||8/22 J23100(SpeI&PstI)||--||RBS-tetR-DT||-- | ||
| + | |- | ||
| + | |experiment||8/22 Plac(SpeI&PstI)||8.0||8/18 RBS-lacZα-DT||10 | ||
|} | |} | ||
</div> | </div> | ||
Revision as of 13:16, 22 September 2013
Aug 31
Gel Extraction
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 2 | -- | -- |
| 3 | RBS-lysis2-(9) | EcoRI & SpeI |
| 4 | ||
| 5 | -- | -- |
| 6 | pSB1C3(1) | EcoRI & SpeI |
| 7 | ||
| 8 | -- | -- |
| 9 | pSB1C3(1) | XbaI&PstI |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 2 | tRNA-Spinach | EcoRI & SpeI |
| 3 | ||
| 4 | -- | -- |
| 5 | pT181 attenuator-(2) | EcoRI & SpeI |
| 6 | ||
| 7 | pT181 attenuator-(2) | XbaI & PstI |
| 8 | ||
| 9 | -- | -- |
| 10 | pT181 antisense-(2) | EcoRI & SpeI |
| 11 |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 2 | pT181 antisense | XbaRI & PstI |
| 3 | ||
| 4 | -- | -- |
| 5 | tetR aptamer 12_1R-(1) | EcoRI & SpeI |
| 6 |
| Name | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| RBS-lysis2(EcoRI&SpeI) | 4.0 | 2.69 | 0.24 |
| pSB1C3(EcoRI&SpeI) | 6.0 | 2.48 | 0.05 |
| pSB1C3(XbaI&PstI) | -- | -- | -- |
| tRNA-Spinach | -- | -- | -- |
| pT181 attenuator-(2)(EcoRI&SpeI) | -- | -- | -- |
| pT181 anttenuator-(2)(SbaI&PstI) | -- | -- | -- |
| pT181 antisense-(2)(EcoRI&SpeI) | -- | -- | -- |
| pT181 antisense-(2)(XbaI&PstI) | -- | -- | -- |
| tetR aptamer 12_1R(EcoRI&SpeI) | -- | -- | -- |
Ligation
| state | Vector | Inserter | Ligation High ver.2 | ||
|---|---|---|---|---|---|
| experiment | 8/30 pSB1C3(EcoRI&SpeI) | 6.6 | 8/30 tRNA-Spinach(EcoRI&SpeI) | 12.5 | 3.5 |
| experiment | 8/30 pSB1C3(EcoRI&SpeI) | 6.6 | 8/30 pT181 antisense(EcoRI&SpeI) | 12.5 | 3.5 |
| experiment | 8/30 pSB1C3(XbaI&Pst) | 12.5 | 8/30 pT181 attenuator(XbaI&PstI) | 25 | 3.5 |
| experiment | 8/30 pSB1C3(XbaI&Pst) | 12.5 | 8/30 pT181 antisense(XbaI&PstI) | 25 | 3.5 |
| experiment | 8/20 DT | 6.6 | 8/30 pT181 attenuator(2) | 12.5 | 3.5 |
| experiment | 8/20 DT | 6.6 | 8/30 pT181 antisense | 12.5 | 3.5 |
| experiment | 8/27 DT | 3.0 | 8/30 tRNA-Spinach(EcoRI&SpeI) | 12.5 | 3.5 |
| experiment | 8/20 DT | 6.6 | 8/30 RBS-lysis2 | 12.5 | 3.5 |
| experiment | 8/22 J23100(SpeI&PstI) | -- | RBS-tetR-DT | -- | |
| experiment | 8/22 Plac(SpeI&PstI) | 8.0 | 8/18 RBS-lacZα-DT | 10 | |
