Team:Imperial College/data
From 2013.igem.org
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<h2>Waste Assessment</h2> | <h2>Waste Assessment</h2> | ||
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[[File:Waste_cocktail.png|thumbnail|center|600px|<b>(A)</b> WCM precursor material, this sterilised media made from LB and SRF was used to produce all WCM utilised. <b>(B)</b> Cells containing mCherry pigment grown in SRF <b>(A)</b> over 3 days, then streaked in a qualitative assay to check for growth. <b>(C)</b> mCherry cells were streaked again after 7 days growth in SRF.]] | [[File:Waste_cocktail.png|thumbnail|center|600px|<b>(A)</b> WCM precursor material, this sterilised media made from LB and SRF was used to produce all WCM utilised. <b>(B)</b> Cells containing mCherry pigment grown in SRF <b>(A)</b> over 3 days, then streaked in a qualitative assay to check for growth. <b>(C)</b> mCherry cells were streaked again after 7 days growth in SRF.]] | ||
[[File:PBS_Plus_Waste.jpg|thumbnail|center|600px|<b>(A)</b> SRF in PBS (phosphate buffered saline), a buffer. We can see from this experiment whether our bacteria can grow solely on the waste SRF. <b>(B)</b> Cells containing mCherry pigment grown in SRF <b>(A)</b> over 3 days, then streaked in a qualitative assay to check for growth. <b>(C)</b> mCherry cells were streaked again after 6 days growth in SRF.]] | [[File:PBS_Plus_Waste.jpg|thumbnail|center|600px|<b>(A)</b> SRF in PBS (phosphate buffered saline), a buffer. We can see from this experiment whether our bacteria can grow solely on the waste SRF. <b>(B)</b> Cells containing mCherry pigment grown in SRF <b>(A)</b> over 3 days, then streaked in a qualitative assay to check for growth. <b>(C)</b> mCherry cells were streaked again after 6 days growth in SRF.]] | ||
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Revision as of 20:07, 22 September 2013
Contents |
Growth Assays
Media characterisation
All medias tested
LB
M9 Minimal
M9 Supplemented
Waste Conditioned Media
Waste Assessment
pBAD characterisation
Glucose
Product inhibition
L-lactic Acid
Ethylene glycol
3-hydroxybutyrate (3HB)
Acetoacetate
Poly(3-hydroxybutyrate) P(3HB)
Poly(lactic acid) (PLA)
IPTG induction assay
Originally we intended on using [http://parts.igem.org/Part:BBa_K639003 BBa_K639003] to detect whether our cells were stressed when placed in various toxic byproducts. However, as the data below shows, this biobrick is very leaky. As such, we are using the stress sensor as a marker for cell growth and also to show that the cells had been successfully transformed with the correct chloramphenicol resistance.
Sole carbon source
3HB
Acetoacetate
Induction
Empty Vector Control
Western blots
Enzyme Kinetics
PHB production