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From 2013.igem.org

Revision as of 01:40, 23 September 2013 (view source) Franer (Talk | contribs) (Created page with "<div id="external"> Two 100 ml culture of EColi DH5alfa carrying a plasmid with a mRFP generator under arsenite sensitive promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_...") ← Older edit		Revision as of 01:43, 23 September 2013 (view source) Franer (Talk | contribs) (Replaced content with "<div id="external"> holaslskad </div>") Newer edit →
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-	Two 100 ml culture of EColi DH5alfa carrying a plasmid with a mRFP generator under arsenite sensitive promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) where grown at 30°C until they reach OD=0.4 (600nm). At this point arsenite was added (10ppb and 1000ppb of arsenite final concentration in each other) fluorescence was measured every 30 minutes for 8 hours with a fluorimeter at 584 nm excitation peak and 607 nm emission peak. All these data was normalized by the culture density measured by OD.	+	holaslskad
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Revision as of 01:43, 23 September 2013