Template:Kyoto/Notebook/Sep 1

(Difference between revisions)

Revision as of 05:45, 23 September 2013

No name

Name	Sample	Competent Cells	Total	Plate
8/31 tRNA-Spinach(pSB1C3)	2µL	20µL	22µL	CP
8/31 pT181 antisense(pSB1C3)	2µL	20µL	22µL	CP
8/31 pT181 attenuator(pSB1C3)	2µL	20µL	22µL	CP
8/31 pT181 antisense(pSB1C3)	2µL	20µL	22µL	CP
8/31 pT181 attenuator(2)-DT	2µL	20µL	22µL	CP
8/31 pT181 antisense-DT	2µL	20µL	22µL	CP
8/31 tRNA-Spniach-DT	2µL	20µL	22µL	CP
8/31 RBS-lysis2-DT	2µL	20µL	22µL	CP
8/31 Pconst-RBS-tetR-DT	2µL	20µL	22µL	Amp
8/31 Plac-RBS-lacZα-DT	2µL	20µL	22µL	CP
8/31 Ptet-RBS-lacZα-DT	2µL	20µL	22µL	CP
8/31	2µL	20µL	22µL	CP

No name

PreDenature	Denature	Annealing	Extension	cycle
94°C	94°C	55°C	68°C	--
5min	30s	30s	30min	30cycle

Nakamoto

state	Vector		Inserter
experiment	DT(E+S)	5.5	8/28 RBS-lysis1(E+S)2.4ng/µL	2.4

1. Samples were evaporeted used evaporator into about 7 µL.
2. Add Ligation High 3.5&micro:L and incubate 16°C 1hour.

Tatsui,Stephane,Nakamoto

No name 5xM9 liquid medium

1. mix 5xM9 medium and 3.75%Agar solution 400mL
2. Add 1M MgSO4, 2M Glucose and 1 VitaminB1 0.5mL
3. Add CaCl2 while shaking Erlenmeyer flask.

DNA	concentration[µg/mL]	260/280	260/230
8/21 pSB1C3(1)	309.2	1.69	1.72
8/21 pSB1C3(2)	201.6	1.65	1.76

Hirano

Add 20µL Kanamycin into 80µL MilliQ and apply it on a 9/1 M9 plate

Hirano

Apply entA-colony liquid culture (100µL) on M9(Km)plate and incubate 37°C

2 fold diluted OD 600 2.107

@@ Line 75: / Line 75: @@
 </div>
-===Master Plate===
+===Liquid Culture===
 <div class="experiment">
-<span class="author">Tatsui,Stephane</span>
+<span class="author">Tatsui,Stephane,Nakamoto</span>
 {| class="wikitable"
-!Number||Use LB plate (+抗生物質)
+!Sample||medium
 |-
-| ʅ（´◔౪◔）ʃ||
+|8/21 pSB1C3(BBa_J04450)-(1)||Plusgrow medium(+CP)
-|}
+|-
-</div>
+|8/21 pSB1C3(BBa_J04450)-(2)||Plusgrow medium(+CP)
+|-
+|RBS-lysis3-DT||Plusgrow medium(+CP)
+|}
-===Liquid Culture===
-<div class="experiment">
-<span class="author">Tatsui,Stephane,Nakamoto</span>
 ===M9 Medium===