Template:Kyoto/Notebook/Sep 2

From 2013.igem.org

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===Colony PCR===
===Colony PCR===
<div class="experiment">
<div class="experiment">
-
<span class="author">No name</span>
+
<span class="author">Tatsui</span>
 +
{| class="wikitable"
 +
!|Sample||base pair
 +
|-
 +
|9/1 RBS-lysis2-DT-(1)||985
 +
|-
 +
|Ptet-RBS-lacZα-DT-(1)||765
 +
|-
 +
|Plac-RBS-lacZα-DT-(1)||765
 +
|-
 +
|Plac-RBS-lacZα-DT-(2)||765
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
 +
|-
 +
|5min||30s||30s||1min||30cycles
 +
|}
 +
[[File:igku_sep2electrophoresis1.png]]
</div>
</div>

Revision as of 05:59, 23 September 2013

Contents

Sep 2

Liquid Culture

Hirano

Samplemedium
9/1 entA-(Master Plate)-14mL SOB(+Km)

37°C

Colony PCR

Tatsui

Samplebase pair
9/1 RBS-lysis2-DT-(1)985
Ptet-RBS-lacZα-DT-(1)765
Plac-RBS-lacZα-DT-(1)765
Plac-RBS-lacZα-DT-(2)765
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min30cycles

File:Igku sep2electrophoresis1.png

Restriction Enzyme Digestion

Nakamoto

Liquid Culture

Tatsui,Stephane,Nakamoto

Master Plate

No name

Gel Extraction

EDTA Solution

Hirano

Colony PCR

Hirano

Master Plate

Hirano

Liquid Culture

Hirano

Colony PCR

Hirano

Transformation

Hirano
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