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Template:Kyoto/Notebook/Sep 4
From 2013.igem.org
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(→Sep 4) |
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| + | </div> | ||
| + | |||
| + | ===Colony PCR=== | ||
| + | <div class="experiment"> | ||
| + | <span class="author">Kojima</span> | ||
| + | {| class="wikitable" | ||
| + | !|Sample||base pair | ||
| + | |- | ||
| + | |8/18 J23100 control -(1)||1142 | ||
| + | |- | ||
| + | |8/18 J23100-RBS-GFP-DT -(1)||1156 | ||
| + | |- | ||
| + | |8/18 J23100-RBS-GFP-DT -(2)||1156 | ||
| + | |- | ||
| + | |8/18 J23100-RBS-luxR-DT -(1)||1271 | ||
| + | |- | ||
| + | |8/18 J23100-RBS-luxR-DT -(2)||1271 | ||
| + | |- | ||
| + | |8/18 J23100-RBS-lacZα-DT -(1)||670 | ||
| + | |- | ||
| + | |8/18 J23100-RBS-lacZα-DT -(2)||670 | ||
| + | |- | ||
| + | |8/18 RBS-lysis3 -(1)||997 | ||
| + | |- | ||
| + | |8/18 RBS-lysis3 -(2)||997 | ||
| + | |- | ||
| + | |8/18 P0440(RBS-tetR-DT) -(1)||1154 | ||
| + | |- | ||
| + | |8/18 P0440(RBS-tetR-DT) -(2)||1154 | ||
| + | |- | ||
| + | |negative control||-- | ||
| + | |} | ||
| + | {| class="wikitable" | ||
| + | !PreDenature||Denature||Annealing||Extension||cycle | ||
| + | |- | ||
| + | |94°C||94°C||55°C||68°C||-- | ||
| + | |- | ||
| + | |5min||30s||30s||1min||30cycles | ||
| + | |} | ||
| + | [[File:igku_Aug19electrophoresis1.png]] | ||
| + | <br> | ||
| + | {| class="wikitable" | ||
| + | !Sample||base pair | ||
| + | |- | ||
| + | |8/9 BBa-J23100 3||-- | ||
| + | |- | ||
| + | |8/9 BBa-J23100 4||-- | ||
| + | |- | ||
| + | |8/9 BBa-J23100 5||-- | ||
| + | |- | ||
| + | |} | ||
| + | {| class="wikitable" | ||
| + | !PreDenature||Denature||Annealing||Extension||cycle | ||
| + | |- | ||
| + | |94°C||94°C||55°C||68°C||-- | ||
| + | |- | ||
| + | |5min||30s||30s||30s||30cycles | ||
| + | |} | ||
| + | [[File:igku_Aug19electrophoresis2.png]] | ||
</div> | </div> | ||
Revision as of 12:14, 23 September 2013
Contents |
Sep 4
Gel Extraction
1
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 2 | Pcon | Spe1&Pst1 |
| 3 | Pcon | Spe1&Pst1 |
| 4 | -- | -- |
| 5 | Plac | Spe1&Pst1 |
| 6 | Plac | Spe1&Pst1 |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| （´ω｀） | ||||
| （ΦωΦ^） |
</div>
2
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 2 | Ptet | Spe1&Pst1 |
| 3 | Ptet | Spe1&Pst1 |
| 4 | -- | -- |
| 5 | RBS-tetR-DT | Xba1&Pst1 |
| 6 | RBS-tetR-DT | Xba1&Pst1 |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| （´ω｀） | ||||
| （ΦωΦ^） |
Master Plate
| Number | Use LB plate(+Amp) |
|---|---|
| 4 | 8/9 BBa-J23100-3 |
| 5 | 8/9 BBa-J23100-4 |
| 6 | 8/9 BBa-J23100-5 |
Master Plate
| Number | Use LB plate |
|---|---|
| 1 | JM109-1 |
| 2 | JM109-2 |
| 3 | JM109-3 |
| 4 | JM109-4 |
Liquid Culture
| Sample | medium |
|---|---|
| 8/9 BBa-J23100-3 | Plusgrow medium(+Amp) |
Liquid Culture
| Sample | medium |
|---|---|
| JM109-1(Master plate) | LB medium |
Colony PCR
| Sample | base pair |
|---|---|
| 8/18 J23100 control -(1) | 1142 |
| 8/18 J23100-RBS-GFP-DT -(1) | 1156 |
| 8/18 J23100-RBS-GFP-DT -(2) | 1156 |
| 8/18 J23100-RBS-luxR-DT -(1) | 1271 |
| 8/18 J23100-RBS-luxR-DT -(2) | 1271 |
| 8/18 J23100-RBS-lacZα-DT -(1) | 670 |
| 8/18 J23100-RBS-lacZα-DT -(2) | 670 |
| 8/18 RBS-lysis3 -(1) | 997 |
| 8/18 RBS-lysis3 -(2) | 997 |
| 8/18 P0440(RBS-tetR-DT) -(1) | 1154 |
| 8/18 P0440(RBS-tetR-DT) -(2) | 1154 |
| negative control | -- |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94°C | 94°C | 55°C | 68°C | -- |
| 5min | 30s | 30s | 1min | 30cycles |
File:Igku Aug19electrophoresis1.png
| Sample | base pair |
|---|---|
| 8/9 BBa-J23100 3 | -- |
| 8/9 BBa-J23100 4 | -- |
| 8/9 BBa-J23100 5 | -- |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94°C | 94°C | 55°C | 68°C | -- |
| 5min | 30s | 30s | 30s | 30cycles |
