Team:UNITN-Trento/Notebook/Labposts/08/41

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{"date" : "2013-08-18","author" : "thomas","title" : "Preparing sequencing samples of AraC-pBAD EFE","content" : "This morning I got to prepares samples of AraC-pBAD EFE to send them for sequencing. We decided to sequence both samples with Vf and Vr primers. So knowing the preps concentrations, I prepared the mix as follow:

Quantification results

	Volume added to sample 1:1 (µl)	Volume added to sample 1:2 (µl)
Template DNA	5.5	6.5
Primer	2	2
Water	up to 15	up to 15

The samples were then putted in a falcon and send with a courier. ","tags" : "aracpbad-efe"}