Team:UNITN-Trento/Notebook/Labposts/08/41
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(Created page with "{"date" : "2013-08-18","author" : "thomas","title" : "<html>Preparing sequencing samples of AraC-pBAD EFE</html>","content" : "<html>This morning I got to prepares samples of Ara...") |
Revision as of 12:17, 23 September 2013
{"date" : "2013-08-18","author" : "thomas","title" : "Preparing sequencing samples of AraC-pBAD EFE","content" : "This morning I got to prepares samples of AraC-pBAD EFE to send them for sequencing. We decided to sequence both samples with Vf and Vr primers. So knowing the preps concentrations, I prepared the mix as follow:
Quantification results
The samples were then putted in a falcon and send with a courier. ","tags" : "aracpbad-efe"}
Volume added to sample 1:1 (µl) | Volume added to sample 1:2 (µl) | |
---|---|---|
Template DNA | 5.5 | 6.5 |
Primer | 2 | 2 |
Water | up to 15 | up to 15 |