Team:NCTU Formosa

E.colightuner

Using sRNA, temperature and light, we want to construct a complicated regulation system. First, we took advantage of a powerful regulated molecules, sRNA. Bacterial small RNAs(sRNA) are small non-coding molecules, and they are highly structured and contain several stem-loops. sRNAs can either bind to protein targets, and modify the function of the bound protein , or bind to mRNA targets and regulate gene expression. Second, we used a Ribosome binding site which can sense change of temperature, 37oC RBS. Because this sequence has a unique secondary structure, it can’t function in room temperature. When temperature increasing, the secondary structure would change and can function. Third, we designed a new promoter, Pred. It is activated by red light. When removing the red light, it would be turned off, so we use this promoter to strengthen our project. By these important factors, we can achieve the pathway regulation and do more applications.