Template:Kyoto/Notebook/Sep 12

From 2013.igem.org

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==Sep 12==
==Sep 12==
 +
===Miniprep===
 +
<div class="experiment">
 +
<span class="author">Nakamoto</span>
 +
{|class="wikitable"
 +
!DNA||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|Pcon-pT181atte-DT||293.6　||1.88　||1.84
 +
|-
 +
|Pcon-apt12-1R-DT||303.8　||1.75　||1.64
 +
|-
 +
|Fusion1atte(pSB1c3)||172.0　||1.96　||2.31
 +
|
 +
|RBS-lisis1-DT||235.1||1.59||1.60
 +
|
 +
| RBS-lisis2-DT||387.7||1.91||2.01
 +
|
 +
| RBS-lisis3-DT||387.9||1.91||2.09
 +
|
 +
|Plux||166.7||1.98||1.63
 +
|}
 +
</div>
 +
 +
===Electrophoresis===
 +
<div class="experiment">
 +
<span class="author">Nakamoto</span>
 +
{| class="wikitable"
 +
!Lane||Sample
 +
|-
 +
|1||100bp ladder
 +
|-
 +
|2||9/11 P-RBS-lisis1-DT(Colony PCR prodution)
 +
|-
 +
|}
 +
[[File:igku_xxxxxx.xxx]]<br>
 +
===Liquid Culture===
 +
<div class="experiment">
 +
<span class="author">Nakamoto</span>
 +
{| class="wikitable"
 +
!Sample||medium
 +
|-
 +
|9/10 Pcon-apt12-1R-DT1||Plusgrow medium(+Amp)
 +
|-
 +
|9/10 DT-Pcon-pT181atte1||Plusgrow medium(+CP)
 +
|-
 +
|9/10 Fusion1-atte(pSB1c3)||Plusgrow medium(+CP)　
 +
|}
 +
</div>v>
 +
 +
===Electrophoresis===
 +
<div class="experiment">
 +
<span class="author">Tatsui</span></div>
 +
 +
1
 +
{| class="wikitable"
 +
!Lane||Sample
 +
|-
 +
|1||1kb ladder
 +
|-
 +
|2||pSB4K5(EcoR1&Spe1)|-
 +
|3|| pSB4K5 NC(EcoR1&Spe1)
 +
|-
 +
|4||RBS-lacz-DT(EcoR1&Spe1)|-
 +
|5|| EcoR1&Spe1  NC( EcoR1&Spe1)|-
 +
|6||Pcon-RBS-lacz-DT( EcoR1&Spe1)
 +
|-
 +
|7|| Pcon-RBS-lacz-DT  NC( EcoR1&Spe1)|-
 +
|8||1kb ladder
 +
|-
 +
}
 +
[[File:igku_xxxxxx.xxx]]<br>
 +
 +
===Electrophoresis===
 +
<div class="experiment">
 +
<span class="author">Tatsui</span></div>
 +
 +
2
 +
{| class="wikitable"
 +
!Lane||Sample
 +
|-
 +
|1||1kb ladder
 +
|-
 +
|2||Ptet-pT181anti(Spe1&Pst1) |-
 +
|3|| Ptet-pT181anti NC(Spe1&Pst1)
 +
|-
 +
|4||RBS-lysis2-DT(Xba1&Pst1)|-
 +
|5|| EcoR1&Spe1  NC( EcoR1&Spe1)|-
 +
|6||--
 +
|-
 +
|7|| --|
 +
|8||1kb ladder
 +
|-
 +
}
 +
[[File:igku_xxxxxx.xxx]]<br>

Revision as of 13:33, 23 September 2013

Contents

Sep 12

Miniprep

Nakamoto

DNAconcentration[µg/mL]260/280260/230
Pcon-pT181atte-DT293.6　1.88　1.84
Pcon-apt12-1R-DT303.8　1.75　1.64
Fusion1atte(pSB1c3)172.0　1.96　2.31 RBS-lisis1-DT235.11.591.60 RBS-lisis2-DT387.71.912.01 RBS-lisis3-DT387.91.912.09 Plux166.71.981.63

Electrophoresis

Nakamoto

LaneSample
1100bp ladder
29/11 P-RBS-lisis1-DT(Colony PCR prodution)

File:Igku xxxxxx.xxx

Liquid Culture

Nakamoto

Samplemedium
9/10 Pcon-apt12-1R-DT1Plusgrow medium(+Amp)
9/10 DT-Pcon-pT181atte1Plusgrow medium(+CP)
9/10 Fusion1-atte(pSB1c3)Plusgrow medium(+CP)　
v>

Electrophoresis

Tatsui

1

} File:Igku xxxxxx.xxx

Electrophoresis

Tatsui

2

LaneSample
11kb ladder
2- 3 pSB4K5 NC(EcoR1&Spe1)
4- 5- 6Pcon-RBS-lacz-DT( EcoR1&Spe1)
7- 81kb ladder
} File:Igku xxxxxx.xxx
LaneSample
11kb ladder
2- 3 Ptet-pT181anti NC(Spe1&Pst1)
4- 5- 6--
7 81kb ladder
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