Template:Kyoto/Notebook/Aug 17

From 2013.igem.org

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(Aug 17)
(Colony PCR)
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===Aug 17===
===Aug 17===
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===Colony PCR===
 
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<div class="experiment">
 
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<span class="author">Nakamoto</span>
 
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{| class="wikitable"
 
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!|Sample||base pair
 
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|-
 
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|8/15 BBa_K112806(lysis2) -(1)||828
 
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|-
 
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|negative control||--
 
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|}
 
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{| class="wikitable"
 
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!PreDenature||Denature||Annealing||Extension||cycle
 
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|-
 
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|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
 
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|-
 
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|5min||30s||30s||1min||30cycles
 
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|}
 
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<!-- [[File:image]] -->
 
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{| class="wikitable"
 
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!Sample||base pair
 
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|-
 
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|8/10 BBa_Q0400 -(1)||3995
 
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|-
 
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|negative control||--
 
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|}
 
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{| class="wikitable"
 
-
!PreDenature||Denature||Annealing||Extension||cycle
 
-
|-
 
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|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
 
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|-
 
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|5min||30s||30s||30s||30cycles
 
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|}
 
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[[File:igku_Aug19electrophoresis2.png]]
 
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</div>
 
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<br>
 
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===Colony PCR===
===Colony PCR===
<div class="experiment">
<div class="experiment">

Revision as of 13:36, 23 September 2013

Aug 17

Colony PCR

Nakamoto

Samplebase pair
8/15 BBa_K112806(lysis2) -(1)828
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min30cycles
Samplebase pair
8/10 BBa_Q0400 -(1)3995
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s30s30cycles

File:Igku Aug19electrophoresis2.png