Template:Kyoto/Notebook/Sep 12

From 2013.igem.org

(Difference between revisions)
(→Sep 12)
(→Electrophoresis)
Line 58: Line 58:
|1||1kb ladder
|1||1kb ladder
|-
|-
-
|2||pSB4K5(EcoR1&Spe1)|-
+
|2||pSB4K5(EcoR1&Spe1)
 +
|-
|3|| pSB4K5 NC(EcoR1&Spe1)
|3|| pSB4K5 NC(EcoR1&Spe1)
|-
|-
-
|4||RBS-lacz-DT(EcoR1&Spe1)|-
+
|4||RBS-lacz-DT(EcoR1&Spe1)
-
|5|| EcoR1&Spe1  NC( EcoR1&Spe1)|-
+
|-
 +
|5|| EcoR1&Spe1  NC( EcoR1&Spe1)
 +
|-
|6||Pcon-RBS-lacz-DT( EcoR1&Spe1)
|6||Pcon-RBS-lacz-DT( EcoR1&Spe1)
|-
|-
-
|7|| Pcon-RBS-lacz-DT  NC( EcoR1&Spe1)|-
+
|7|| Pcon-RBS-lacz-DT  NC( EcoR1&Spe1)
 +
|-
|8||1kb ladder
|8||1kb ladder
|-
|-
Line 84: Line 88:
|6||--
|6||--
|-
|-
-
|7|| --|
+
|7|| --|-
|8||1kb ladder
|8||1kb ladder
|-
|-
}
}
[[File:igku_xxxxxx.xxx]]<br>
[[File:igku_xxxxxx.xxx]]<br>

Revision as of 13:37, 23 September 2013

Contents

Sep 12

Miniprep

Nakamoto

DNAconcentration[µg/mL]260/280260/230
Pcon-pT181atte-DT293.6　1.88　1.84
Pcon-apt12-1R-DT303.8　1.75　1.64
Fusion1atte(pSB1c3)172.0　1.96　2.31
RBS-lisis1-DT235.11.591.60
RBS-lisis2-DT387.71.912.01
RBS-lisis3-DT387.91.912.09
Plux166.71.981.63

Electrophoresis

Nakamoto

LaneSample
1100bp ladder
29/11 P-RBS-lisis1-DT(Colony PCR prodution)

File:Igku xxxxxx.xxx

Liquid Culture

Nakamoto

Samplemedium
9/10 Pcon-apt12-1R-DT1Plusgrow medium(+Amp)
9/10 DT-Pcon-pT181atte1Plusgrow medium(+CP)
9/10 Fusion1-atte(pSB1c3)Plusgrow medium(+CP)　
v>

Electrophoresis

Tatsui

1

} File:Igku xxxxxx.xxx
2
LaneSample
11kb ladder
2pSB4K5(EcoR1&Spe1)
3 pSB4K5 NC(EcoR1&Spe1)
4RBS-lacz-DT(EcoR1&Spe1)
5 EcoR1&Spe1 NC( EcoR1&Spe1)
6Pcon-RBS-lacz-DT( EcoR1&Spe1)
7 Pcon-RBS-lacz-DT NC( EcoR1&Spe1)
81kb ladder
} File:Igku xxxxxx.xxx
LaneSample
11kb ladder
2- 3 Ptet-pT181anti NC(Spe1&Pst1)
4- 5- 6--
7- 81kb ladder
Retrieved from "http://2013.igem.org/Template:Kyoto/Notebook/Sep_12"