Template:Kyoto/Notebook/Aug 17
From 2013.igem.org
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+ | </div> | ||
+ | ===Master Plate=== | ||
+ | <div class="experiment"> | ||
+ | <span class="author">Nakamoto</span> | ||
+ | {| class="wikitable" | ||
+ | !Number||Use LB plate (+CP) | ||
+ | |- | ||
+ | |1||BBa_K112806(lysis2) -(1) | ||
+ | |} | ||
+ | </div> | ||
+ | <div class="experiment"> | ||
+ | {| class="wikitable" | ||
+ | !Number||Use LB plate (+Kana) | ||
+ | |- | ||
+ | |1||BBa_Q04400(tetR) -(1) | ||
+ | |} | ||
</div> | </div> |
Revision as of 14:08, 23 September 2013
Contents |
Aug 17
Colony PCR
Sample | base pair |
---|---|
8/15 BBa_K112806(lysis2) -(1) | 828 |
negative control | -- |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
94°C | 94°C | 55°C | 68°C | -- |
5min | 30s | 30s | 1min | 30cycles |
Sample | base pair |
---|---|
8/10 BBa_Q0400 -(1) | 3995 |
negative control | -- |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
94°C | 94°C | 55°C | 68°C | -- |
5min | 30s | 30s | 30s | 30cycles |
Liquid Culture
Sample | medium |
---|---|
8/15 BBa_K112806(lysis2) -(1) | Plusgrow medium(+CP) |
8/15 BBa_Q04400(tetR) -(1) | Plusgrow medium(+Amp) |
Master Plate
Number | Use LB plate (+CP) |
---|---|
1 | BBa_K112806(lysis2) -(1) |
Number | Use LB plate (+Kana) |
---|---|
1 | BBa_Q04400(tetR) -(1) |