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Template:Kyoto/Notebook/Sep 12
From 2013.igem.org
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[[File:igku_xxxxxx.xxx]] | [[File:igku_xxxxxx.xxx]] | ||
| + | |||
| + | ===Gel Extraction=== | ||
| + | <div class="experiment"> | ||
| + | <span class="author">Tatsui</span> | ||
| + | {| class="wikitable" | ||
| + | !Lane||DNA||Enzyme | ||
| + | |- | ||
| + | |1||1kb ladder||-- | ||
| + | |- | ||
| + | |2||RBS-lacZ-DT||EcoR1&Spe1 | ||
| + | |- | ||
| + | |3||RBS-lacZ-DT||EcoR1&Spe1 | ||
| + | |- | ||
| + | |4||RBS-lacZ-DT||EcoR1&Spe1 | ||
| + | |- | ||
| + | |5||--||-- | ||
| + | | | ||
| + | |6||Pcon-RBS-lacZ-DT||EcoR1&Spe1 | ||
| + | |- | ||
| + | |7||Pcon-RBS-lacZ-DT||EcoR1&Spe1 | ||
| + | |- | ||
| + | |8||Pcon-RBS-lacZ-DT||EcoR1&Spe1 | ||
| + | |- | ||
| + | |9||1kb ladder | ||
| + | |} | ||
| + | [[File:igku_Aug19electrophoresis3]]<br> | ||
| + | [[File:igku_Aug19electrophoresis4]]<br> | ||
| + | {| class="wikitable" | ||
| + | !Name||concentration[µg/mL]||260/280||260/230 | ||
| + | |- | ||
| + | |8/18 lysis2-1(XbaI & PstI)||3.1||3.64||0.19 | ||
| + | |- | ||
| + | |8/18 lysis2-1(XbaI & PstI)||3.4||1.89||0.13 | ||
| + | |} | ||
| + | |||
| + | </div> | ||
Revision as of 14:17, 23 September 2013
Contents |
Sep 12
Miniprep
| DNA | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| Pcon-pT181atte-DT | 293.6 | 1.88 | 1.84 |
| Pcon-apt12-1R-DT | 303.8 | 1.75 | 1.64 |
| Fusion1atte(pSB1c3) | 172.0 | 1.96 | 2.31 |
| RBS-lisis1-DT | 235.1 | 1.59 | 1.60 |
| RBS-lisis2-DT | 387.7 | 1.91 | 2.01 |
| RBS-lisis3-DT | 387.9 | 1.91 | 2.09 |
| Plux | 166.7 | 1.98 | 1.63 |
Electrophoresis
| Lane | Sample |
|---|---|
| 1 | 100bp ladder |
| 2 | 9/11 P-RBS-lisis1-DT(Colony PCR prodution) |
Liquid Culture
| Sample | medium |
|---|---|
| 9/10 Pcon-apt12-1R-DT1 | Plusgrow medium(+Amp) |
| 9/10 DT-Pcon-pT181atte1 | Plusgrow medium(+CP) |
| 9/10 Fusion1-atte(pSB1c3) | Plusgrow medium(+CP) |
Electrophoresis
1
| Lane | Sample |
|---|---|
| 1 | 1kb ladder |
| 2 | pSB4K5(EcoR1&Spe1) |
| 3 | pSB4K5 NC(EcoR1&Spe1) |
| 4 | RBS-lacz-DT(EcoR1&Spe1) |
| 5 | EcoR1&Spe1 NC( EcoR1&Spe1) |
| 6 | Pcon-RBS-lacz-DT( EcoR1&Spe1) |
| 7 | Pcon-RBS-lacz-DT NC( EcoR1&Spe1) |
| 8 | 1kb ladder |
2
| Lane | Sample |
|---|---|
| 1 | 1kb ladder |
| 2 | Ptet-pT181anti(Spe1&Pst1) |
| 3 | Ptet-pT181anti NC(Spe1&Pst1) |
| 4 | RBS-lysis2-DT(Xba1&Pst1) |
| 5 | EcoR1&Spe1 NC( EcoR1&Spe1) |
| 6 | -- |
| 7 | -- |
| 8 | 1kb ladder |
Gel Extraction
| Lane | DNA | Enzyme | ||||
|---|---|---|---|---|---|---|
| 1 | 1kb ladder | -- | ||||
| 2 | RBS-lacZ-DT | EcoR1&Spe1 | ||||
| 3 | RBS-lacZ-DT | EcoR1&Spe1 | ||||
| 4 | RBS-lacZ-DT | EcoR1&Spe1 | ||||
| 5 | -- | -- | 6 | Pcon-RBS-lacZ-DT | EcoR1&Spe1 | |
| 7 | Pcon-RBS-lacZ-DT | EcoR1&Spe1 | ||||
| 8 | Pcon-RBS-lacZ-DT | EcoR1&Spe1 | ||||
| 9 | 1kb ladder |
File:Igku Aug19electrophoresis3
File:Igku Aug19electrophoresis4
| Name | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| 8/18 lysis2-1(XbaI & PstI) | 3.1 | 3.64 | 0.19 |
| 8/18 lysis2-1(XbaI & PstI) | 3.4 | 1.89 | 0.13 |
