Template:Kyoto/Notebook/Sep 12

From 2013.igem.org

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Revision as of 14:21, 23 September 2013

Contents

Sep 12

Miniprep

Nakamoto

DNAconcentration[µg/mL]260/280260/230
Pcon-pT181atte-DT293.6　1.88　1.84
Pcon-apt12-1R-DT303.8　1.75　1.64
Fusion1atte(pSB1c3)172.0　1.96　2.31
RBS-lisis1-DT235.11.591.60
RBS-lisis2-DT387.71.912.01
RBS-lisis3-DT387.91.912.09
Plux166.71.981.63

Electrophoresis

Nakamoto

LaneSample
1100bp ladder
29/11 P-RBS-lisis1-DT(Colony PCR prodution)

File:Igku xxxxxx.xxx

Liquid Culture

Nakamoto

Samplemedium
9/10 Pcon-apt12-1R-DT1Plusgrow medium(+Amp)
9/10 DT-Pcon-pT181atte1Plusgrow medium(+CP)
9/10 Fusion1-atte(pSB1c3)Plusgrow medium(+CP)　
v>

Electrophoresis

Tatsui

1

LaneSample
11kb ladder
2pSB4K5(EcoR1&Spe1)
3 pSB4K5 NC(EcoR1&Spe1)
4RBS-lacz-DT(EcoR1&Spe1)
5 EcoR1&Spe1 NC( EcoR1&Spe1)
6Pcon-RBS-lacz-DT( EcoR1&Spe1)
7 Pcon-RBS-lacz-DT NC( EcoR1&Spe1)
81kb ladder

File:Igku xxxxxx.xxx

2

LaneSample
11kb ladder
2Ptet-pT181anti(Spe1&Pst1)
3 Ptet-pT181anti NC(Spe1&Pst1)
4RBS-lysis2-DT(Xba1&Pst1)
5 EcoR1&Spe1 NC( EcoR1&Spe1)
6--
7--
81kb ladder

File:Igku xxxxxx.xxx

Gel Extraction

Tatsui

LaneDNAEnzyme
11kb ladder--
2RBS-lacZ-DTEcoR1&Spe1
3RBS-lacZ-DTEcoR1&Spe1
4RBS-lacZ-DTEcoR1&Spe1
5----
6Pcon-RBS-lacZ-DTEcoR1&Spe1
7Pcon-RBS-lacZ-DTEcoR1&Spe1
8Pcon-RBS-lacZ-DTEcoR1&Spe1
91kb ladder--

File:Igku Aug19electrophoresis3
File:Igku Aug19electrophoresis4

Nameconcentration[µg/mL]260/280260/230
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