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Template:Kyoto/Notebook/Sep 9
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==Sep 9== | ==Sep 9== | ||
| + | ===PCR=== | ||
| + | <div class="experiment"> | ||
| + | <span class="author">Nakagawa</span> | ||
| + | {| class="wikitable" | ||
| + | !KaiA(plasmid)||10x buffer||dNTPs||MgSO4||Primer(661-RBS-KaiA-fwd)||Primer(kaiA-662-rev-ver2)||Kod-plus ver2||MilliQ||total | ||
| + | |- | ||
| + | |1.0||2.5||2.5||1.5||0.75||0.75||0.5||15.5||25 | ||
| + | |} | ||
| + | {| class="wikitable" | ||
| + | !PreDenature||Denature||Annealing||Extension||cycle | ||
| + | |- | ||
| + | |94°C||98°C||58.5°C||68°C||-- | ||
| + | |- | ||
| + | |2min||10s||30s||30s||30cycles | ||
| + | |} | ||
| + | </div> | ||
| + | <div class="experiment"> | ||
| + | {| class="wikitable" | ||
| + | !KaiB(plasmid)||10x buffer||dNTPs||MgSO4||Primer(661-RBS-KaiA-fwd-ver2)||Primer(kaiB-663-rev-ver2)||Kod-plus ver2||MilliQ||total | ||
| + | |- | ||
| + | |1.0||2.5||2.5||1.5||0.75||0.75||0.5||15.5||25 | ||
| + | |} | ||
| + | {| class="wikitable" | ||
| + | !PreDenature||Denature||Annealing||Extension||cycle | ||
| + | |- | ||
| + | |94°C||98°C||58.5°C||68°C||-- | ||
| + | |- | ||
| + | |2min||10s||30s||30s||30cycles | ||
| + | |} | ||
| + | {| class="wikitable" | ||
| + | !P-KaiBC(PCR products)||10x buffer||dNTPs||MgSO4||Primer(prefix-P-kaiBC-fwd-ver2)||Primer(P-KaiBC-suffix-rev-verw)||Kod-plus ver2||MilliQ||total | ||
| + | |- | ||
| + | |0.7||2.5||2.5||1.5||0.75||0.75||0.5||15.8||25 | ||
| + | |} | ||
| + | {| class="wikitable" | ||
| + | !PreDenature||Denature||Annealing||Extension||cycle | ||
| + | |- | ||
| + | |94°C||98°C||58.5°C||68°C||-- | ||
| + | |- | ||
| + | |2min||10s||30s||30s||30cycles | ||
| + | |} | ||
| + | </div> | ||
Revision as of 17:13, 23 September 2013
Sep 9
PCR
| KaiA(plasmid) | 10x buffer | dNTPs | MgSO4 | Primer(661-RBS-KaiA-fwd) | Primer(kaiA-662-rev-ver2) | Kod-plus ver2 | MilliQ | total |
|---|---|---|---|---|---|---|---|---|
| 1.0 | 2.5 | 2.5 | 1.5 | 0.75 | 0.75 | 0.5 | 15.5 | 25 |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94°C | 98°C | 58.5°C | 68°C | -- |
| 2min | 10s | 30s | 30s | 30cycles |
| KaiB(plasmid) | 10x buffer | dNTPs | MgSO4 | Primer(661-RBS-KaiA-fwd-ver2) | Primer(kaiB-663-rev-ver2) | Kod-plus ver2 | MilliQ | total |
|---|---|---|---|---|---|---|---|---|
| 1.0 | 2.5 | 2.5 | 1.5 | 0.75 | 0.75 | 0.5 | 15.5 | 25 |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94°C | 98°C | 58.5°C | 68°C | -- |
| 2min | 10s | 30s | 30s | 30cycles |
| P-KaiBC(PCR products) | 10x buffer | dNTPs | MgSO4 | Primer(prefix-P-kaiBC-fwd-ver2) | Primer(P-KaiBC-suffix-rev-verw) | Kod-plus ver2 | MilliQ | total |
|---|---|---|---|---|---|---|---|---|
| 0.7 | 2.5 | 2.5 | 1.5 | 0.75 | 0.75 | 0.5 | 15.8 | 25 |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94°C | 98°C | 58.5°C | 68°C | -- |
| 2min | 10s | 30s | 30s | 30cycles |
