Template:Kyoto/Notebook/Sep 8

From 2013.igem.org

(Difference between revisions)
(→Sep 8)
(→Gel Extraction)
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|2||RBS-lysis1-DT||X+P
|2||RBS-lysis1-DT||X+P
|-
|-
-
|3|||RBS-lysis1-DT||X+P
+
|3||RBS-lysis1-DT||X+P
|-
|-
-
|5|||RBS-lysis3-DT||X+P
+
|5||RBS-lysis3-DT||X+P
|-
|-
-
|6|||RBS-lysis3-DT||X+P
+
|6||RBS-lysis3-DT||X+P
|}
|}
[[File:igku_xxbeforexx.xxx]]<br>
[[File:igku_xxbeforexx.xxx]]<br>
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!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
|-
|-
-
|（´ω｀）|| || || ||
+
|RBS-lysis1-DT(X+P)|| ||5.3||1.98||0.06
|-
|-
-
|（ΦωΦ^）|| || || ||
+
|RBS-lysis3-DT(X+P)|| ||5.3||1.98||0.06
|}
|}
</div>
</div>
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!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
|-
|-
-
|（´ω｀）|| || || ||
+
|Pcun-pT181 atte(E+S)|| ||5.2||1.82||0.36
|-
|-
-
|（ΦωΦ^）|| || || ||
+
|Spinach-DT(X+P)|| ||8.0||1.87||0.25
 +
|-
 +
|RBS-lacZα-DT(X+P)|| ||10.3||1.71||0.54
|}
|}
{| class="wikitable"
{| class="wikitable"
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|1||1kbp ladder||
|1||1kbp ladder||
|-
|-
-
|2||Ptet-anti||E+P
+
|2||Ptet-pT181-anti||E+P
|-
|-
-
|3||Ptet-anti||E+P
+
|3||Ptet-pT181-anti||E+P
|-
|-
|5||pSB4K5||E+S
|5||pSB4K5||E+S
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!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
|-
|-
-
|（´ω｀）|| || || ||
+
|Ptet-pT181-anti(E+P)|| ||30.6||1.84||1.16
|-
|-
-
|（ΦωΦ^）|| || || ||
+
|pSB4K5(E+S)|| ||21.8||1.87||0.98
|}
|}
{| class="wikitable"
{| class="wikitable"
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!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
|-
|-
-
|（´ω｀）|| || || ||
+
|Pcun-pT181 atte(S+P)|| ||14.5||1.91||1.09
 +
|-
 +
|Plac(S+P)|| ||11.6||1.74||1.19
 +
|-
 +
|pSB1C3(E+S)|| ||5.7||1.74||0.34
|-
|-
-
|（ΦωΦ^）|| || || ||
+
|pSB1C3(X+P)|| ||5.6||2.00||0.37
|}
|}
 +
===Miniprep===
===Miniprep===
<div class="experiment">
<div class="experiment">

Revision as of 17:49, 23 September 2013

Contents

Sep 8

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
1100bp ladder
2RBS-lysis1-DTX+P
3RBS-lysis1-DTX+P
5RBS-lysis3-DTX+P
6RBS-lysis3-DTX+P

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
RBS-lysis1-DT(X+P) 5.31.980.06
RBS-lysis3-DT(X+P) 5.31.980.06
LaneDNAEnzyme
1100bp ladder
3Pcun-pT181 atteE+S
4Pcun-pT181 atteE+S
6Spinach-DTX+P
7Spinach-DTX+P
9RBS-lacZα-DTX+P
10RBS-lacZα-DTX+P

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
Pcun-pT181 atte(E+S) 5.21.820.36
Spinach-DT(X+P) 8.01.870.25
RBS-lacZα-DT(X+P) 10.31.710.54
LaneDNAEnzyme
11kbp ladder
2Ptet-pT181-antiE+P
3Ptet-pT181-antiE+P
5pSB4K5E+S
6pSB4K5E+S

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
Ptet-pT181-anti(E+P) 30.61.841.16
pSB4K5(E+S) 21.81.870.98
LaneDNAEnzyme
11kbp ladder
2Pcun-pT181 atteS+P
3Pcun-pT181 atteS+P
5PlacS+P
6PlacS+P
8pSB1C3E+S
9pSB1C3E+S
11pSB1C3X+P
12pSB1C3X+P

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
Pcun-pT181 atte(S+P) 14.51.911.09
Plac(S+P) 11.61.741.19
pSB1C3(E+S) 5.71.740.34
pSB1C3(X+P) 5.62.000.37

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
(・▽・)　　

Ligation

じっけんしゃ

stateVectorInserterLigation High ver.2
experimentnamexx µg/mLnamexx µg/mLxx µg/mL
NCnamexx µg/mLnamexx µg/mLxx µg/mL

Restriction Enzyme Digestion

じっけんしゃ

DNArestricion enzymerestriction enzymeBufferBSAMilliQtotal
2 cuts（°°） µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL

じっけんしゃ

DNArestricion enzymerestriction enzymeBufferBSAMilliQtotal
2 cuts（°°） µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL

Transformation

じっけんしゃ

NameSampleCompetent CellsTotalPlate
いでんしなんちゃらµLごにょごにょµLほにゃららµLこーせーぶっしつ
いかどうよう

Electrophoresis

じっけんしゃ

LaneSampleEnzyme1Enzyme2
（°Д°）
LaneSampleEnzyme1Enzyme2
（°Д°）
LaneSampleEnzyme1Enzyme2
（°Д°）

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）

Ligation

じっけんしゃ

stateVectorInserterLigation High ver.2
experimentnamexx µg/mLnamexx µg/mLxx µg/mL
NCnamexx µg/mLnamexx µg/mLxx µg/mL
stateVectorInserterLigation High ver.2
experimentnamexx µg/mLnamexx µg/mLxx µg/mL
NCnamexx µg/mLnamexx µg/mLxx µg/mL

PCR

No name

genome DNAKOD plus10x bufferdNTPMgSO4SasA_fwd primerSasA_rev primerMilliQtotal
0.52.52.51.50.750.75 25
PreDenatureDenatureAnnealingExtensioncycle
　°C　°C　°C　°C--
（◎Д◎）　　　　

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
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