Template:Kyoto/Notebook/Sep 9

From 2013.igem.org

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!DNA||concentration[µg/mL]||260/280||260/230  
!DNA||concentration[µg/mL]||260/280||260/230  
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|(・▽・)||　||　||
+
|9/8 pSB1C3||83.2|1.45||0.76
 +
|-
 +
|9/8 RBS-lysis2-DT||72.8|1.67||0.97
 +
|-
 +
|9/8 spinach-DT||152.6|1.88||1.97
 +
|-
 +
|Nuclease-Free water||-0.4|-14.30||0.25
|}
|}
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Revision as of 17:52, 23 September 2013

Sep 9

PCR

Nakagawa

KaiA(plasmid)10x bufferdNTPsMgSO4Primer(661-RBS-KaiA-fwd)Primer(kaiA-662-rev-ver2)Kod-plus ver2MilliQtotal
1.02.52.51.50.750.750.515.525
PreDenatureDenatureAnnealingExtensioncycle
94°C98°C58.5°C68°C--
2min10s30s30s30cycles
KaiB(plasmid)10x bufferdNTPsMgSO4Primer(661-RBS-KaiA-fwd-ver2)Primer(kaiB-663-rev-ver2)Kod-plus ver2MilliQtotal
1.02.52.51.50.750.750.515.525
PreDenatureDenatureAnnealingExtensioncycle
94°C98°C58.5°C68°C--
2min10s30s30s30cycles
P-KaiBC(PCR products)10x bufferdNTPsMgSO4Primer(prefix-P-kaiBC-fwd-ver2)Primer(P-KaiBC-suffix-rev-verw)Kod-plus ver2MilliQtotal
0.72.52.51.50.750.750.515.825
PreDenatureDenatureAnnealingExtensioncycle
94°C98°C58.5°C68°C--
2min10s30s30s30cycles
DT(plasmid)10x bufferdNTPsMgSO4Primer(664-DT-fwd-ver2)Primer(DT-suffix-660-rev-ver2)Kod-plus ver2MilliQtotal
1.02.52.51.50.750.750.515.525
PreDenatureDenatureAnnealingExtensioncycle
94°C98°C58.5°C68°C--
2min10s30s30s30cycles

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
9/8 pSB1C31.450.76
9/8 RBS-lysis2-DT1.670.97
9/8 spinach-DT1.881.97
Nuclease-Free water-14.300.25
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