Team:KU Leuven/Project/Aphid Background

Synopsis

During a long period of co-evolution, plants and aphids have established a complex interaction. On the one hand, aphids have established complex life cycles involving extensive phenotype plasticity with rapid population growth (Blackman and Eastop, 1984) and a very short generation time (Morrison and Peairs, 1998). On the other hand, plants have also evolved delicate and complicated defence systems comprising constitutive defence traits and defence pathways induced upon aphid attack (Chen 2008). Breeders and growers are still struggling to find an efficient strategy for aphid control in major crop plants, as the damage can run in the millions. The KU Leuven iGEM project has an answer to this problem. Our project consists of a genetically engineered bacteria that can reduce the aphid damage on a sustainable way by hacking into their communication system.

Basic aphid biology

Aphids are an extremely successful group of insects which occurs throughout the world, with the greatest number of species in the temperate regions: Europe, North America and Asia (Dixon et al, 1987). As individuals they are small and inconspicuous. However, they can become so numerous that they can damage whole fields or orchards. The complex life cycles and polymorphism enable them to exploit their host plants and respond to every alteration of their environment to a high degree (Biology of Aphids, Dixon, 1973). Aphids are small, mostly soft-bodied insects of the super family Aphidoidea (World crop pests, 2A, Aphids their biology, natural enemies and control, Minks and Harrewijn). Their body can be green, black brown, pink or almost colourless (Biology of aphids, Dixon, 1973). About 4400 species of 10 families are known and around 10% of these species are serious pests for agriculture and forestry (Dedryver et al, 2010). Annual worldwide crop losses due to aphids are estimated at hundreds of millions of dollars (Morrison and Peairs 1998).

How aphids feed on plants

The head of aphids is pointed downward for feeding. They use modified mouth parts, stylets, to obtain their food by sucking plant juices (World crop pests, 2A, Aphids their biology, natural enemies and control, minks and harrewijn). Aphids passively feed on phloem sap of the plants. Once a phloem vessel, which is under high pressure, is punctured, the sap is forced into the aphid’s food canal. Phloem sap is rich in sugars, but poor in amino acids, which are essential for growth. Therefore, aphids ingest a very large amount of food in order to acquire sufficient proteins. The residual solution of digested food is ejected into the exterior in the form of a droplet of honeydew (Biology of Aphids, Dixon, 1973). This sugary liquid is produced through a ‘tail’ at the rear end of the aphid, called a cauda (World crop pests, 2A, Aphids their biology, natural enemies and control, minks and harrewijn). Most aphids also have paired tubular structures found at the end of the abdomen, called cornicles (Siphunculus). Through these cornicles, aphids excrete droplets of a quick-hardening defensive fluid containing triacylglycerols, the cornicle wax. Other defensive compounds, like alarm pheromones, can also be secreted by the cornicles (aphid, McGraw-Hill Encyclopedia of Science and Technology).

Life cycles

Aphids display a diverse range of complicated life cycles (World crop pests, 2A, Aphids their biology, natural enemies and control, minks and harrewijn). Many aphid species are monophagous, they feed on only one plant species. The economically important aphids tend to have a wider host range and are polyphagous (Blackman and Eastop, 1985). They have a primary woody plant during winter and a secondary herbaceous plant in the summer (Dixon et al, 1994). In addition, aphid species have evolved a wide range of annual cycles and adaptive mechanisms that can vary between and within species (Biology of aphids, Dixon, 1973). Most aphids are parthenogenetic and viviparous for most of the year, but are also capable of sexual reproduction with the production of eggs during the summer. The annual cycle generally (97%) includes a single sexual generation. These mating females (oviparae) lay eggs, all of which are female. On hatching, each egg gives rise to a wingless offspring that gives birth parthenogenetically to further parhenogenetic females (viviparae). These may be winged or wingless, in response to environmental conditions (biology of aphids, Dixon, 1973). Crowding is a particularly strong stimulus to develop aphids of the winged, dispersal stage. Other external factors also play a role in the morph determination: food availability and quality, predators, photoperiod, time of the year and so on. In conclusion, aphids can rapidly exploit agricultural landscapes because of their high reproductive potential, dispersal capacities, adaptability to local environment, short generation time and feed on an endless source of plants (Dedryver et al, 2010).

Damage to plants and solutions

Aphid damage to plants may be caused directly, by nutrient drain, or indirectly by several mechanisms. These indirect mechanisms include plant virus transmission through the aphids’ piercing-sucking mouthparts, injection of saliva containing phytotoxic components that have a disastrous effect on plant growth and harmful fungi growth on the aphids’ honeydew hindering photosynthetic activity. Common damage symptoms on plants are decreased growth rates, mottled leaves, yellowing, stunted growth, curled leaves, gall formation and low yields and death (Biology of Aphids, Dixon, 1973). Different methods can be used to control an aphid infestation. For many crops, insecticides provide a simple and effective strategy for aphid control. However, the application of such chemicals is not desirable in the long term, because of the development of insecticide resistance and the potential negative effects on the population dynamics of other beneficial insects (World crop pests, 2A, Aphids their biology, natural enemies and control, minks and harrewijn). Aphids are vulnerable to many kinds of predators, including ladybird beetles and parasitoid wasps, and parasites and these can also be used to control aphids (World crop pests, 2A, Aphids their biology, natural enemies and control, minks and harrewijn).

Synopsis

Attraction of insects to plants and other host organisms involves detection of specific semiochemicals (natural signal chemicals mediating changes in behavior and development) or specific rations of these semiochemicals (Pickett et al, 2006). Plants colonized and damaged by herbivorous insects produce a group of volatile organic compounds, herbivore-induced plant volatiles. These compounds act through induced direct and indirect defenses on the herbivore pests (Lou et al, 2006). The direct defenses include chemicals that act as repellents for herbivorous pests. The indirect defenses increase the herbivore mortality through the recruitment of parasitoids and predators (Hatano et al, 2008).

Prey localization

Predatory and parasitoid insects have a specialized sensory nervous system to detect their prey (Hatano et al, 2008). They use volatiles produced by the herbivores, reliable but at low concentration, or those produced by the plant to locate their prey. These latter are easily detectable, but are less reliable. To overcome the reliablility-detecability problem predators and parasitoids focus on the responses on stimuli created by specific interactions between the herbivore and its plant (Vet & Dicke, 1992). In response to an aphid attack, plants modify their volatile emissions and these changes are detected by aphid enemies (Du et al, 1998). Host selection occurs in three phases: habitat localization, host localization and host acceptance (Vinson, 1976). Aphid natural enemies must first locate the aphid habitat, the host plant where aphids are present. Therefore, plant-derived volatiles are used, since evidence of aphid damage is acquired. One of the important herbivore-induced plant volatiles that are used by predators is methyl salicylate (MeS) (Zu & Park, 2005). The secretion of MeS is induced by the feeding of aphids on the plant (Birkett et al, 2000). Following habitat localization, natural enemies use short range physical (colour, shape, movement of aphid) and chemical cues to search for a suitable herbivore on the host plant (Hatano et al, 2008). One of the chemical cues used by natural enemies is aphid honeydew. Mostly, the natural enemies need physical contact with honeydew to change their behavior (Ide et al, 2007). In addition to aphid honeydew, the aphid alarm pheromone, (E)-β-Farnesene (EBF) is also an important semiochemicals in aphid localization. It is released in the cornicle secretions of many aphid species (Franscis et al, 2005) to alert surrounding aphids of the presence of natural enemies (Kunert et al, 2005). Detection of these short range chemical cues leads not to the aphid directly, but only indicates the presence, improving prey detection of the predators and parasitoids. Once an aphid is located, natural enemies have to recognize it as a potential prey before they attack it. For host recognition, non volatile chemical cues are important, in particular contact with the cornicle wax on the surface of the aphids. Predators use their antennae or their mouthparts to recognize the prey. Parasitoids use probing to assess host quality before oviposition (Hatano et al, 2008).

β-Farnesene

Aphid populations are regulated by natural enemies such as ladybird beetles and parasitoid wasps (World crop pests, 2A, Aphids their biology, natural enemies and control, minks and harrewijn). For many pest species of aphids, avoidance of predators involves the release of an alarm pheromone. (E)-β-Farnesene (EβF), a volatile sesquiterpene, is released from the cornicles on the aphids’ abdomen when attacked by its natural enemies (Dixon 1998). Therefore, EβF functions as a direct repellent and acts as a kairomone (chemical messengers emitted by organisms of one species but benefit members of another species) for attraction of natural enemies of aphids (Hatano et al. 2008).
The high oxidation rate of EβF has hampered the application of EβF to cop plants against aphids (Hille Ris Lambers and Schepers 1978). We get around this problem by using E. coli to produce the aphids’ own alarm pheromone (EβF) and use them right on the plants. Beside using the aphid communication, we also want to attract aphid predators such as ladybugs to make sure the aphids are thoroughly removed.

Methyl Salicylate

When plants are attacked by herbivore insects, they emit a volatile signal that is dependent on the type of insect (Vlot et al, 2008). Herbivore-induced plant volatiles mediate relationships between plants and insects throught the attraction of natural enemies and the repulsion of herbivores (Turlings and Ton, 2006). Manipulation of herbivore-induced plant volatiles can be used to concentrate and increase populations of natural enemies in a specific place, such as crop fields, or to repel pests from crop plants (Khan et al, 2008). Methyl salicylate (MeS) is a phenolic compound that is released when plants are attacked by herbivore insects (Reymond and Farmer, 1998). Upon the activation of the salicylic acid pathway, liquid MeS increases in concentration within a plant, the volatile form is subsequently released in high concentrations (Vlot et al, 2008). Furthermore, MeS itself also induces the salicylic acid pathway and can therefore elicit responses in neighboring healty plants (Heidel and Baldwin, 2004). MeS is also involved in plant defense, it functions as an activator of the defense-related genes.

Green peach aphid

In our experiments, we used Myzus persicae or the green peach aphid, a small generalist aphid that can be found worldwide. The green peach aphid attacks plants in the field as well as in greenhouses. Their primary overwintering host is Prunus sp. (peach trees and their hybrids). However, in the summer, they leave their priary host to feed on a wide range of plants including vegetable crops, weeds and ornamentals.
Temperature greatly influence the lifecycle of the green peach aphid, especially cold winters. Aphids overwinter in the egg stage on Prunus trees. As temperatures warm, the aphid may go through multiple generations while still on the tree, but as densities increase, winged adults are produced. These winged forms colonize nearby plants by depositing daughters on one plant and then moving on to the next plant. Each daughter begins asexual reproduction (parthenogenesis) by rapidly giving birth to new daughters, which often already have developing daughter inside them ready to emerge in a few days. As densities increase on the newly colonized plants, and as the plants deteriorate, new winged forms are produced. This cycle repeats as long as the weather is favourable. Eventually, as day length begins to shorten and temperatures change, the winged females search for Prunus trees to colonize. Females arrive on the overwintering locations first and give birth to wingless egg laying forms (oviparae), which mate with winged males.

Adalia bipunctata

In our experiments we used the two-spotted ladybug. Adalia bipunctata, commonly known as the two-spotted ladybug, is a carnivorous beetle of the family Coccinellidae that is found in western and central Europe and North America. The two-spotted ladybeetle feed on aphids and other small insects. The wing covers of adult two-spotted lady beetles are bright-red with one black spot on each side (Marshall, 2000). Its life cycle starts with bright yellow eggs and ladybugs pass through four larval stages. The larvae and the adults are predators. The larvae of older ages move actively on the whole plant for the search of food and also migrate to other plants. The adult individuals keep not far from preys where they mate and lay the eggs. Therefore, ladybugs are frequently used in ornamentals, fruit and vegetable crops as beneficial to control aphids, for example in greenhouses.

Birkett, M.A., Campbell, C.A.M., Chamberlain, K., Guerrieri, E., Hick, A.J., Martin, J.L., Matthes, M., Napier, J.A., Pettersson, J., Pickett, J.A., Poppy, G.M., Pow, E.M., Pye, B.J., Smart, L.E., Wadhams, G.H., Wadhams, L.J. and Woodcock, C.M. (2000). New roles for cis-jasmone as an insect semiochemicals and in plant defense. PNAS 97:9329-9334
Blackman, R.L., and Eastop, V.F. (1985). Aphids of the world crops: an identification guide. Wiley, Chichester, United Kingdom
Chen, M. (2008). Inducible direct plant defense against insect herbivores a review. Insect Sci. 15:101–114 Dedryver, C.A., Le Ralec, A., and Fabre, F. (2010). The conflicting relationships between aphids and men: a review of aphid damage and control strategies. ComptesRendusBiologies 333:539–553
Dixon, A.F.G. (1973). Biology of aphids. Edward Arnold, London, United Kingdom
Dixon, A.F.G. (1998). Aphid Ecology (2nd edition). Chapman and Hall, London, United Kingdom
Dixon, A.F.G. (2005). Insect predator-prey dynamics: ladybird beetles and biological control. Cambridge University Press, Cambridge, United Kingdom
Dixon, A.F.G., Kindlmann, P., Leps, J., and Holman, J. (1987). Why are there so few species of aphids, especially in the tropics. Amer. Nat. 129:580-592
Du, Y.J., Poppy, G.M., Powell, W., Pickett, J.A., Wadhams, L.J., and Woodcock, C.M. (1998). Identification of semiochemicals released during aphid feeding that attractparasitoidAphidiuservi. J. Chem. Ecol. 24:1355-1368
Francis, F., Martin, T., Lognay, G., and Haubruge, E. (2005).Role of (E)-b-farnesene in systematic aphid prey location by Episyrphusbalteatus larvae (Diptera: Syrphidae). European J. of Entomol., 102:431-436
Francis, F., Vandermoten, S., Verheggen, F.,Lognay, G., and Haubruge, E. (2004). Is the (E)-β-farnesene only volatile terpenoid in aphids? J. of Applied Entomol. 129 (1):6-11
Hatano, E., Kunert, G., Michaud, J.P., and Weisser, W.W. (2008). Chemical cues mediating aphid location by natural enemies. Eur. J. Entomol. 105:797-806
Heidel, A.H., and Baldwin, I.T. (2004). Microarray analysis of salicylic acid and jasmonic acid signalling in responses of Nicotiana attenuate to attack by insects from multiple feeding guilds. Plant Cell Environ., 27:1362-1373
Hodek, I., and Honek., A. (1996). Ecology of Coccinellidae. Kluwer Academic Publishers, Dordrecht, The Netherlands
Ide, T., Suzuki, N., and Katayama, N. (2007).The use of honeydew in foraging for aphids by larvae of the ladybird beetle, Coccinellaseptempunctata L. (Coleoptera: Coccinellidae). Ecol. Entomol. 32:455-460
Khan, Z.R., Midega, C.A.O., Njuguna, E.M., Arnudavi, D.M., Wanyama, J.M., and Pickett, J.A. (2008).Economic performance of the ‘push-pull’ technology for stemborer and striga control in smallholder farming systems in western Kenya. Crop. Prot., 27:1084-1097
Kunert, G., Otto, S., Röse, S.R., Gershenzon, J., and Weisser, W.W. (2005). Alarm pheromones mediates production of winged dispersal morphs in aphids. Ecology letters, 8:596-603
Lambers, D. H. R., and Schepers, A. (1978).Effect of trans-beta-farnesene, used as a repellant against landing aphid alatae in seed potato growing. Potato Res. 21:23-26
Luo, Q., Nakic, M., Wheatley, T., Richell, R., Martin, A., and Blair, R.J. (2006). The neural basis of implicit moral attitude - an IAT study using event-related fMRI. NeuroImage 30:1449–1457
Minks, A.K. and Harrewijn, P. (1987). Aphids: Their biology, natural enemies and control. Elsevier Science Publishers B.V., Amsterdam, The Netherlands
Mordvilko, A.K. (1928). The evolution of cycles and the origin of heteroecy (migration) in plant-lice. Annals and magazine of natural history (serie 10) 2:570-582
Morrison, W. P. and F. B. Peairs (1998). Introduction: Response model concept and economic impact. In: Quisenberry, S.S., and Peairs, F.B. (eds) Response model for an introduced pest – the Russian Wheat Aphid. Thomas Say Publications, Entomological Society of America, Lanham, Maryland, pp. 1-11
Pickett, J.A., Wadhams, L.J., Woodcock, C.M. and Hardie, J. (1992). The chemical ecology of aphids.Annu. Rev. Entomol. 37:67-90
Reymond, P., and Farmer, E.E. (1998). Jasmonate and salicylate as global signals for defense gene expression. Curr. Opin. Plant Biol., 1:404-411
Shingleton, A.W., Sisk, G.C., and Stern, D.L. (2003). Diapause in the pea aphid (Acyrthosiphonpisum) is a slowing but not a cessation of development. BMC Developmental Biology 3(7)
Turlings, T.C.J., and Ton, J. (2006). Exploiting scents of distress: the prospect of manipulating herbivore-induced plant odors to enhance the control of a agricultural pests. Curr. Opin. Plant Biol., 9:421-427
Van Emden, H.F., Eastop, V.F., Hughes, R.D., and Way, M.J. (1969). The Ecology of Myzuspersicae.Annu. Rev. Entomol., 14:197-270
Vet, L.E.M., and Dicke, M. (1992).Ecology of infochemical use by natural enemies in a tritrophic context.Annu. Rev. Entomol. 37:141-172
Vinson, S.B. (1976). Host selection by insect parasitoids.Annu. Rev. Entomol. 21:109-133
Vlot, A.C., Klessig, D.F., and Park, S.W. (2008). Systemic acquired resistance: the elusive signal(s). Curr. Opin. Plant Biol., 11:436-442
Zhu, J.W., and Park, K.C. (2005). Methyl salicylate, a soybean aphid-induced plant volatile attractive to the predator Coccinellaseptempunctata. J. Chem. Ecol. 31:1733-1746