Template:Kyoto/Notebook/Sep 17

From 2013.igem.org

(Difference between revisions)
(Liquid Culture)
(PCR)
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<!-- ここから -->
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<div class="experiment">
<div class="experiment">
-
<span class="author">No name</span>
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<span class="author">Hirano</span>
{| class="wikitable"
{| class="wikitable"
-
!genome DNA||KOD plus||10x buffer||dNTP||MgSO4||SasA_fwd primer||SasA_rev primer||MilliQ||total
+
!Pcon-tet ap-DT(VF)||BigDye||5x buffer||primer(VF)||MilliQ||total
|-
|-
-
| ||0.5||2.5||2.5||1.5||0.75||0.75|| ||25
+
|1.3||0.5||1.75||0.5||0.45||10.5
|}
|}
{| class="wikitable"
{| class="wikitable"
!PreDenature||Denature||Annealing||Extension||cycle
!PreDenature||Denature||Annealing||Extension||cycle
|-
|-
-
| &deg;C|| &deg;C|| &deg;C|| &deg;C||--
+
|96&deg;C||96&deg;C||50&deg;C||60&deg;C||--
|-
|-
-
|(◎Д◎) || || || ||
+
|2m||10s||5s||4m||30cycles
 +
|}
 +
</div>
 +
<!-- ここまでをコピーしてね --><!-- ここから -->
 +
<div class="experiment">
 +
{| class="wikitable"
 +
!Pcon-tet ap-DT(VR)||BigDye||5x buffer||primer(VR)||MilliQ||total
 +
|-
 +
|1.3||0.5||1.75||0.5||0.45||10.5
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|96&deg;C||96&deg;C||50&deg;C||60&deg;C||--
 +
|-
 +
|2m||10s||5s||4m||30cycles
|}
|}
</div>
</div>
<!-- ここまでをコピーしてね -->
<!-- ここまでをコピーしてね -->

Revision as of 01:59, 24 September 2013

Sep 17

Liquid Culture

Tatsui and Hirano

Samplemedium
Spi-DTLB medium(+CP)
Pcon-Spi-DT,Pcon-pT181-anti-spi-DTLB medium(+Amp) 

PCR

Hirano

Pcon-tet ap-DT(VF)BigDye5x bufferprimer(VF)MilliQtotal
1.30.51.750.50.4510.5
PreDenatureDenatureAnnealingExtensioncycle
96°C96°C50°C60°C--
2m10s5s4m30cycles
Pcon-tet ap-DT(VR)BigDye5x bufferprimer(VR)MilliQtotal
1.30.51.750.50.4510.5
PreDenatureDenatureAnnealingExtensioncycle
96°C96°C50°C60°C--
2m10s5s4m30cycles