Template:Kyoto/Notebook/Sep 17
From 2013.igem.org
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(→PCR) |
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<!-- ここから --> | <!-- ここから --> | ||
<div class="experiment"> | <div class="experiment"> | ||
- | <span class="author"> | + | <span class="author">Hirano</span> |
{| class="wikitable" | {| class="wikitable" | ||
- | ! | + | !Pcon-tet ap-DT(VF)||BigDye||5x buffer||primer(VF)||MilliQ||total |
|- | |- | ||
- | | ||0.5|| | + | |1.3||0.5||1.75||0.5||0.45||10.5 |
|} | |} | ||
{| class="wikitable" | {| class="wikitable" | ||
!PreDenature||Denature||Annealing||Extension||cycle | !PreDenature||Denature||Annealing||Extension||cycle | ||
|- | |- | ||
- | | | + | |96°C||96°C||50°C||60°C||-- |
|- | |- | ||
- | | | + | |2m||10s||5s||4m||30cycles |
+ | |} | ||
+ | </div> | ||
+ | <!-- ここまでをコピーしてね --><!-- ここから --> | ||
+ | <div class="experiment"> | ||
+ | {| class="wikitable" | ||
+ | !Pcon-tet ap-DT(VR)||BigDye||5x buffer||primer(VR)||MilliQ||total | ||
+ | |- | ||
+ | |1.3||0.5||1.75||0.5||0.45||10.5 | ||
+ | |} | ||
+ | {| class="wikitable" | ||
+ | !PreDenature||Denature||Annealing||Extension||cycle | ||
+ | |- | ||
+ | |96°C||96°C||50°C||60°C||-- | ||
+ | |- | ||
+ | |2m||10s||5s||4m||30cycles | ||
|} | |} | ||
</div> | </div> | ||
<!-- ここまでをコピーしてね --> | <!-- ここまでをコピーしてね --> |
Revision as of 01:59, 24 September 2013
Sep 17
Liquid Culture
Sample | medium |
---|---|
Spi-DT | LB medium(+CP) |
Pcon-Spi-DT,Pcon-pT181-anti-spi-DT | LB medium(+Amp) |
PCR
Pcon-tet ap-DT(VF) | BigDye | 5x buffer | primer(VF) | MilliQ | total |
---|---|---|---|---|---|
1.3 | 0.5 | 1.75 | 0.5 | 0.45 | 10.5 |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
96°C | 96°C | 50°C | 60°C | -- |
2m | 10s | 5s | 4m | 30cycles |
Pcon-tet ap-DT(VR) | BigDye | 5x buffer | primer(VR) | MilliQ | total |
---|---|---|---|---|---|
1.3 | 0.5 | 1.75 | 0.5 | 0.45 | 10.5 |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
96°C | 96°C | 50°C | 60°C | -- |
2m | 10s | 5s | 4m | 30cycles |