Template:Kyoto/Notebook/Sep 17
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+ | </div> | ||
+ | <!-- ここまでをコピーしてね --> | ||
+ | ===Ligation=== | ||
+ | |||
+ | <!-- こっから --> | ||
+ | <div class="experiment"> | ||
+ | <span class="author">じっけんしゃ</span> | ||
+ | {| class="wikitable" | ||
+ | !state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2 | ||
+ | |- | ||
+ | |experiment||name||xx µg/mL||name||xx µg/mL||xx µg/mL | ||
+ | |- | ||
+ | |NC||name||xx µg/mL||name||xx µg/mL||xx µg/mL | ||
|} | |} | ||
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<!-- ここまでをコピーしてね --> | <!-- ここまでをコピーしてね --> |
Revision as of 02:02, 24 September 2013
Contents |
Sep 17
Liquid Culture
Sample | medium |
---|---|
Spi-DT | LB medium(+CP) |
Pcon-Spi-DT,Pcon-pT181-anti-spi-DT | LB medium(+Amp) |
PCR
Pcon-tet ap-DT(VF) | BigDye | 5x buffer | primer(VF) | MilliQ | total |
---|---|---|---|---|---|
1.3 | 0.5 | 1.75 | 0.5 | 0.45 | 10.5 |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
96°C | 96°C | 50°C | 60°C | -- |
2m | 10s | 5s | 4m | 30cycles |
Pcon-tet ap-DT(VR) | BigDye | 5x buffer | primer(VR) | MilliQ | total |
---|---|---|---|---|---|
1.3 | 0.5 | 1.75 | 0.5 | 0.45 | 10.5 |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
96°C | 96°C | 50°C | 60°C | -- |
2m | 10s | 5s | 4m | 30cycles |
Ligation
state | Vector | Inserter | Ligation High ver.2 | ||
---|---|---|---|---|---|
experiment | name | xx µg/mL | name | xx µg/mL | xx µg/mL |
NC | name | xx µg/mL | name | xx µg/mL | xx µg/mL |