Template:Kyoto/Notebook/Sep 17

From 2013.igem.org

(Difference between revisions)
(PCR)
(PCR)
Line 41: Line 41:
|-
|-
|2m||10s||5s||4m||30cycles
|2m||10s||5s||4m||30cycles
 +
|}
 +
</div>
 +
<!-- ここまでをコピーしてね -->
 +
===Ligation===
 +
 +
<!-- こっから -->
 +
<div class="experiment">
 +
<span class="author">じっけんしゃ</span>
 +
{| class="wikitable"
 +
!state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2
 +
|-
 +
|experiment||name||xx &micro;g/mL||name||xx &micro;g/mL||xx &micro;g/mL
 +
|-
 +
|NC||name||xx &micro;g/mL||name||xx &micro;g/mL||xx &micro;g/mL
|}
|}
</div>
</div>
<!-- ここまでをコピーしてね -->
<!-- ここまでをコピーしてね -->

Revision as of 02:02, 24 September 2013

Contents

Sep 17

Liquid Culture

Tatsui and Hirano

Samplemedium
Spi-DTLB medium(+CP)
Pcon-Spi-DT,Pcon-pT181-anti-spi-DTLB medium(+Amp) 

PCR

Hirano

Pcon-tet ap-DT(VF)BigDye5x bufferprimer(VF)MilliQtotal
1.30.51.750.50.4510.5
PreDenatureDenatureAnnealingExtensioncycle
96°C96°C50°C60°C--
2m10s5s4m30cycles
Pcon-tet ap-DT(VR)BigDye5x bufferprimer(VR)MilliQtotal
1.30.51.750.50.4510.5
PreDenatureDenatureAnnealingExtensioncycle
96°C96°C50°C60°C--
2m10s5s4m30cycles

Ligation

じっけんしゃ

stateVectorInserterLigation High ver.2
experimentnamexx µg/mLnamexx µg/mLxx µg/mL
NCnamexx µg/mLnamexx µg/mLxx µg/mL