Template:Kyoto/Notebook/Sep 17
From 2013.igem.org
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<div class="experiment"> | <div class="experiment"> | ||
- | <span class="author"> | + | <span class="author">No Name</span> |
{| class="wikitable" | {| class="wikitable" | ||
!state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2 | !state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2 | ||
|- | |- | ||
- | | | + | |8/28 ||name||xx µg/mL||name||xx µg/mL||xx µg/mL |
|- | |- | ||
|NC||name||xx µg/mL||name||xx µg/mL||xx µg/mL | |NC||name||xx µg/mL||name||xx µg/mL||xx µg/mL | ||
+ | |} | ||
+ | </div> | ||
+ | <!-- ここまでをコピーしてね --> | ||
+ | ===Gel Extraction=== | ||
+ | <!-- こっから --> | ||
+ | <div class="experiment"> | ||
+ | <span class="author">No Name</span> | ||
+ | {| class="wikitable" | ||
+ | !Lane||DNA||Enzyme | ||
+ | |- | ||
+ | |(>ω・)b || || | ||
+ | |- | ||
+ | | || || | ||
+ | |} | ||
+ | [[File:igku_xxbeforexx.xxx]]<br> | ||
+ | [[File:igku_xxafterxx.xxx]]<br> | ||
+ | {| class="wikitable" | ||
+ | !Name||concentration[µg/mL]||260/280||260/230 | ||
+ | |- | ||
+ | |(´ω`)|| || || | ||
+ | |- | ||
+ | |(ΦωΦ^)|| || || | ||
|} | |} | ||
</div> | </div> | ||
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Revision as of 02:05, 24 September 2013
Contents |
Sep 17
Liquid Culture
Sample | medium |
---|---|
Spi-DT | LB medium(+CP) |
Pcon-Spi-DT,Pcon-pT181-anti-spi-DT | LB medium(+Amp) |
PCR
Pcon-tet ap-DT(VF) | BigDye | 5x buffer | primer(VF) | MilliQ | total |
---|---|---|---|---|---|
1.3 | 0.5 | 1.75 | 0.5 | 0.45 | 10.5 |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
96°C | 96°C | 50°C | 60°C | -- |
2m | 10s | 5s | 4m | 30cycles |
Pcon-tet ap-DT(VR) | BigDye | 5x buffer | primer(VR) | MilliQ | total |
---|---|---|---|---|---|
1.3 | 0.5 | 1.75 | 0.5 | 0.45 | 10.5 |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
96°C | 96°C | 50°C | 60°C | -- |
2m | 10s | 5s | 4m | 30cycles |
Ligation
state | Vector | Inserter | Ligation High ver.2 | ||
---|---|---|---|---|---|
8/28 | name | xx µg/mL | name | xx µg/mL | xx µg/mL |
NC | name | xx µg/mL | name | xx µg/mL | xx µg/mL |
Gel Extraction
Lane | DNA | Enzyme |
---|---|---|
(>ω・)b | ||
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
Name | concentration[µg/mL] | 260/280 | 260/230 |
---|---|---|---|
(´ω`) | |||
(ΦωΦ^) |