Template:Kyoto/Notebook/Sep 17

From 2013.igem.org

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|2||rowspan="3"|Pt181 atte -(2)||rowspan="3"|E+S
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!Name||concentration[µg/mL]||260/280||260/230
!Name||concentration[µg/mL]||260/280||260/230
|-
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|（´ω｀）|| || ||
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|PT181-atte E+S||9.2||1.15||0.12
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</div>
</div>
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{| class="wikitable"
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!Lane||DNA||Enzyme
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|-
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|1||1kbpladder||--
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|-
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|2||rowspan="3"|pSB1C3||rowspan="3"|E+S
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|3
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|4
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[[File:igku_xxbeforexx.xxx]]<br>
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[[File:igku_xxafterxx.xxx]]<br>
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{| class="wikitable"
 +
!Name||concentration[&micro;g/mL]||260/280||260/230
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|-
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|pSB1C3||10.0||1.84||0.40
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|}
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{| class="wikitable"
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!Lane||DNA||Enzyme
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|-
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|1||1kbpladder||--
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|-
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|2||rowspan="3"|pSB4K5||rowspan="3"|X+P
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|-
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|3
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|-
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|4
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|}
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[[File:igku_xxbeforexx.xxx]]<br>
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[[File:igku_xxafterxx.xxx]]<br>
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{| class="wikitable"
 +
!Name||concentration[&micro;g/mL]||260/280||260/230
 +
|-
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|pSB4K5||7.6||1.81||0.32
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|}
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{| class="wikitable"
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!Lane||DNA||Enzyme
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|-
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|1||1kbpladder||--
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|-
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|2||rowspan="3"|Pcon-RBS-tetR-DT||rowspan="3"|X+P
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|-
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|3
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|-
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|4
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|}
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[[File:igku_xxbeforexx.xxx]]<br>
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[[File:igku_xxafterxx.xxx]]<br>
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{| class="wikitable"
 +
!Name||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|Pcon-RBS-tetR-DT||8.0||2.40||1.14
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|}
 +
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{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||1kbpladder||--
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|-
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|2||rowspan="3"|Pcon-RBS-tetR-DT||rowspan="3"|X+P
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|-
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|3
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|-
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|4
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|}
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[[File:igku_xxbeforexx.xxx]]<br>
 +
[[File:igku_xxafterxx.xxx]]<br>
 +
{| class="wikitable"
 +
!Name||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|Pcon-RBS-tetR-DT||8.0||2.40||1.14
 +
|}
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Revision as of 02:25, 24 September 2013

Contents

Sep 17

Liquid Culture

Tatsui and Hirano

Samplemedium
Spi-DTLB medium(+CP)
Pcon-Spi-DT,Pcon-pT181-anti-spi-DTLB medium(+Amp)　

PCR

Hirano

Pcon-tet ap-DT(VF)BigDye5x bufferprimer(VF)MilliQtotal
1.30.51.750.50.4510.5
PreDenatureDenatureAnnealingExtensioncycle
96°C96°C50°C60°C--
2m10s5s4m30cycles
Pcon-tet ap-DT(VR)BigDye5x bufferprimer(VR)MilliQtotal
1.30.51.750.50.4510.5
PreDenatureDenatureAnnealingExtensioncycle
96°C96°C50°C60°C--
2m10s5s4m30cycles

Ligation

No Name

stateVectorInserterLigation High ver.2
8/28 namexx µg/mLnamexx µg/mLxx µg/mL
NCnamexx µg/mLnamexx µg/mLxx µg/mL

Gel Extraction

No Name

LaneDNAEnzyme
11kbpladder--
2Pt181 atte -(2)E+S
3
4

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
PT181-atte E+S9.21.150.12
LaneDNAEnzyme
11kbpladder--
2pSB1C3E+S
3
4

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
pSB1C310.01.840.40
LaneDNAEnzyme
11kbpladder--
2pSB4K5X+P
3
4

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
pSB4K57.61.810.32
LaneDNAEnzyme
11kbpladder--
2Pcon-RBS-tetR-DTX+P
3
4

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
Pcon-RBS-tetR-DT8.02.401.14
LaneDNAEnzyme
11kbpladder--
2Pcon-RBS-tetR-DTX+P
3
4

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
Pcon-RBS-tetR-DT8.02.401.14
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