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Template:Kyoto/Notebook/Sep 17
From 2013.igem.org
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Revision as of 02:26, 24 September 2013
Contents |
Sep 17
Liquid Culture
| Sample | medium |
|---|---|
| Spi-DT | LB medium(+CP) |
| Pcon-Spi-DT,Pcon-pT181-anti-spi-DT | LB medium(+Amp) |
PCR
| Pcon-tet ap-DT(VF) | BigDye | 5x buffer | primer(VF) | MilliQ | total |
|---|---|---|---|---|---|
| 1.3 | 0.5 | 1.75 | 0.5 | 0.45 | 10.5 |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 96°C | 96°C | 50°C | 60°C | -- |
| 2m | 10s | 5s | 4m | 30cycles |
| Pcon-tet ap-DT(VR) | BigDye | 5x buffer | primer(VR) | MilliQ | total |
|---|---|---|---|---|---|
| 1.3 | 0.5 | 1.75 | 0.5 | 0.45 | 10.5 |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 96°C | 96°C | 50°C | 60°C | -- |
| 2m | 10s | 5s | 4m | 30cycles |
Ligation
| state | Vector | Inserter | Ligation High ver.2 | ||
|---|---|---|---|---|---|
| 8/28 | name | xx µg/mL | name | xx µg/mL | xx µg/mL |
| NC | name | xx µg/mL | name | xx µg/mL | xx µg/mL |
Gel Extraction
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 1kbpladder | -- |
| 2 | Pt181 atte -(2) | E+S |
| 3 | ||
| 4 |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| PT181-atte E+S | 9.2 | 1.15 | 0.12 |
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 1kbpladder | -- |
| 2 | pSB1C3 | E+S |
| 3 | ||
| 4 |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| pSB1C3 | 10.0 | 1.84 | 0.40 |
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 1kbpladder | -- |
| 2 | pSB4K5 | X+P |
| 3 | ||
| 4 |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| pSB4K5 | 7.6 | 1.81 | 0.32 |
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 1kbpladder | -- |
| 2 | Pcon-RBS-tetR-DT | X+P |
| 3 | ||
| 4 |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| Pcon-RBS-tetR-DT | 8.0 | 2.40 | 1.14 |
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 1kbpladder | -- |
| 2 | pSB1C3 | X+P |
| 3 | ||
| 4 |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| pSB1C3 | 9.1 | 2.17 | 0.35 |
