Template:Kyoto/Notebook/Sep 17

(Difference between revisions)

Revision as of 02:27, 24 September 2013

Tatsui and Hirano

Sample	medium
Spi-DT	LB medium(+CP)
Pcon-Spi-DT,Pcon-pT181-anti-spi-DT	LB medium(+Amp)

Hirano

Pcon-tet ap-DT(VF)	BigDye	5x buffer	primer(VF)	MilliQ	total
1.3	0.5	1.75	0.5	0.45	10.5

PreDenature	Denature	Annealing	Extension	cycle
96°C	96°C	50°C	60°C	--
2m	10s	5s	4m	30cycles

Pcon-tet ap-DT(VR)	BigDye	5x buffer	primer(VR)	MilliQ	total
1.3	0.5	1.75	0.5	0.45	10.5

PreDenature	Denature	Annealing	Extension	cycle
96°C	96°C	50°C	60°C	--
2m	10s	5s	4m	30cycles

No Name

state	Vector		Inserter		Ligation High ver.2
8/28	name	xx µg/mL	name	xx µg/mL	xx µg/mL
NC	name	xx µg/mL	name	xx µg/mL	xx µg/mL

No Name

Name	concentration[µg/mL]	260/280	260/230
PT181-atte E+S	9.2	1.15	0.12

Name	concentration[µg/mL]	260/280	260/230
pSB1C3	10.0	1.84	0.40

Name	concentration[µg/mL]	260/280	260/230
pSB4K5	7.6	1.81	0.32

Name	concentration[µg/mL]	260/280	260/230
Pcon-RBS-tetR-DT	8.0	2.40	1.14

Name	concentration[µg/mL]	260/280	260/230
pSB1C3	9.1	2.17	0.35

@@ Line 146: / Line 146: @@
 |1||1kbpladder||--
 |-
-|2||rowspan="3"|pSB1C3||rowspan="3"|X+P
+|2||rowspan="3"|pSB1C3||rowspan="3"|E+P
 |-
 |3