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Template:Kyoto/Notebook/Sep 11
From 2013.igem.org
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!pKaiBC(200p,400p,800p,1ng)||10*buffer KOD plus||dNTPs||MgSO4||primer mix(F&R)||KOD plus||MilliQ||total | !pKaiBC(200p,400p,800p,1ng)||10*buffer KOD plus||dNTPs||MgSO4||primer mix(F&R)||KOD plus||MilliQ||total | ||
|- | |- | ||
| - | | || | + | | ||2.5||2.5||1.5||1.5||0.5||16|| ||25 |
|} | |} | ||
{| class="wikitable" | {| class="wikitable" | ||
Revision as of 02:44, 24 September 2013
Sep 11
| pKaiBC(200p,400p,800p,1ng) | 10*buffer KOD plus | dNTPs | MgSO4 | primer mix(F&R) | KOD plus | MilliQ | total | |
|---|---|---|---|---|---|---|---|---|
| 2.5 | 2.5 | 1.5 | 1.5 | 0.5 | 16 | 25 |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| °C | °C | °C | °C | -- |
| （◎Д◎） |
