Template:Kyoto/Notebook/Sep 11

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(→Sep 11)
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<span class="author">Tatsui</span>
<span class="author">Tatsui</span>
{| class="wikitable"
{| class="wikitable"
-
!pKaiBC(200p,400p,800p,1ng)||10*buffer KOD plus||dNTPs||MgSO4||primer mix(F&R)||KOD plus||MilliQ||total
+
!pKaiBC(200p)||10*buffer KOD plus||dNTPs||MgSO4||primer mix(F&R)||KOD plus||MilliQ||total
|-
|-
| ||2.5||2.5||1.5||1.5||0.5||16|| ||25
| ||2.5||2.5||1.5||1.5||0.5||16|| ||25
Line 10: Line 10:
!PreDenature||Denature||Annealing||Extension||cycle
!PreDenature||Denature||Annealing||Extension||cycle
|-
|-
-
|　&deg;C||　&deg;C||　&deg;C||　&deg;C||--
+
|　94&deg;C||　98&deg;C||　60&deg;C||　68&deg;C||--
|-
|-
-
|（◎Д◎）　||　||　||　||
+
|2min　||10sec　||30sec　||30sec　||
|}
|}
</div>
</div>

Revision as of 02:49, 24 September 2013

Sep 11

Tatsui

pKaiBC(200p)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
2.52.51.51.50.516 25
PreDenatureDenatureAnnealingExtensioncycle
　94°C　98°C　60°C　68°C--
2min　10sec　30sec　30sec　
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