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Template:Kyoto/Notebook/Sep 11
From 2013.igem.org
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!PreDenature||Denature||Annealing||Extension||cycle | !PreDenature||Denature||Annealing||Extension||cycle | ||
|- | |- | ||
| - | | 94°C|| 98°C|| 60°C|| 68°C|| | + | | 94°C|| 98°C|| 60°C|| 68°C||30 |
|- | |- | ||
|2min ||10sec ||30sec ||30sec || | |2min ||10sec ||30sec ||30sec || | ||
|} | |} | ||
</div> | </div> | ||
Revision as of 02:50, 24 September 2013
Sep 11
| pKaiBC(200p) | 10*buffer KOD plus | dNTPs | MgSO4 | primer mix(F&R) | KOD plus | MilliQ | total | |
|---|---|---|---|---|---|---|---|---|
| 2.5 | 2.5 | 1.5 | 1.5 | 0.5 | 16 | 25 |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94°C | 98°C | 60°C | 68°C | 30 |
| 2min | 10sec | 30sec | 30sec |
