Template:Kyoto/Notebook/Sep 11

From 2013.igem.org

(Difference between revisions)
(→Colony PCR)
(→Sep 11)
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==Sep 11==
==Sep 11==
 +
===PCR===
<div class="experiment">
<div class="experiment">
<span class="author">Tatsui</span>
<span class="author">Tatsui</span>
Line 59: Line 60:
===Colony PCR===
===Colony PCR===
 +
<div class="experiment">
 +
<span class="author">tatsui</span>
 +
{| class="wikitable"
 +
!Sample||base pair
 +
|-
 +
|(｀・ω・´)||
 +
|}
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{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
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|　&deg;C||　&deg;C||　&deg;C||　&deg;C||--
 +
|-
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|（◎Д◎）　||　||　||　||
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|}
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</div>

Revision as of 03:08, 24 September 2013

Sep 11

PCR

Tatsui

pKaiBC(200p)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
0.52.52.51.51.50.516 25
PreDenatureDenatureAnnealingExtensioncycle
　94°C　98°C　60°C　68°C30
2min　10sec　30sec　30sec　
pKaiBC(400p)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
12.52.51.51.50.515.5 25
PreDenatureDenatureAnnealingExtensioncycle
　94°C　98°C　60°C　68°C30
2min　10sec　30sec　30sec　
pKaiBC(800p)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
22.52.51.51.50.514.5 25
PreDenatureDenatureAnnealingExtensioncycle
　94°C　98°C　60°C　68°C30
2min　10sec　30sec　30sec　
pKaiBC(1ng)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
2.52.52.51.51.50.514 25
PreDenatureDenatureAnnealingExtensioncycle
　94°C　98°C　60°C　68°C30
2min　10sec　30sec　30sec　

Colony PCR

tatsui

Samplebase pair
(｀・ω・´)
PreDenatureDenatureAnnealingExtensioncycle
　°C　°C　°C　°C--
（◎Д◎）　　　　
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