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Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/4.5PhageSpotTest
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(Created page with "{{TeamBYUProvo}} <br> {| width="100%" | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification March - April Notebook: Experiments'''</font> <...") |
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: 1x LB top agar | : 1x LB top agar | ||
:Phage: 14 T3 | :Phage: 14 T3 | ||
| - | : | + | : 13 T5 |
| - | : | + | : 10 T2 |
| - | : | + | : 20OX171 |
| - | : | + | : 40ØX174 |
'''IV) Actual Procedure''' | '''IV) Actual Procedure''' | ||
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: We plated together .5mL of BL21 with 1X LB TA. | : We plated together .5mL of BL21 with 1X LB TA. | ||
We separated the plates into different segments and then spot tested the phage. | We separated the plates into different segments and then spot tested the phage. | ||
| - | The plates were then put in a 37°C incubator | + | The plates were then put in a 37°C incubator. |
| + | |||
'''V) Results''' | '''V) Results''' | ||
: No plaques were seen on a uniform bacterial lawn. The control was clear of any contamination or plaques. The phage were not viable. | : No plaques were seen on a uniform bacterial lawn. The control was clear of any contamination or plaques. The phage were not viable. | ||
Revision as of 22:16, 17 June 2013
| Phage Purification March - April Notebook: Experiments
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4.5 Phage Spot Test
I) Purpose
II) Expected Outcome
III) Reagants Used
IV) Actual Procedure
We separated the plates into different segments and then spot tested the phage. The plates were then put in a 37°C incubator. V) Results
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