Template:Kyoto/Notebook/Sep 23

(Difference between revisions)

Revision as of 04:55, 24 September 2013

No Name

Lane	DNA	Enzyme
1	1kbp ladder
3-4	Pcon-RBS-tetR-PT

Name	concentration[µg/mL]	260/280	260/230
Pcon-PT181 anti E+S	46.7	1.06	0.93
Pcon-RBS-tetR-DT E+X	119.3	0.99
RBS-8FP-DT E+X	87.7	1.00	0.98
ap42_1R-DT E+X	68.8	1.32	1.03
Pcon-RBS-tetR-DT 1-2 A	203.0	1.66	1.79
Pcon-RBS-tetR-DT 3-2 A	67.7	2.64	1.28
Pcon-RBS-GFP-DT 1-S A	185.6	1.88	2.04
Pcon-RBS-GFP-DT 2-S A	298.6	1.68	1.55
Pcon-tetR-DT E-2 A	202.4	1.57	1.66

No name

genome DNA	KOD plus	10x buffer	dNTP	MgSO4	SasA_fwd primer	SasA_rev primer	MilliQ	total
9/8 Pcon-pT181 anti	0.5	2.5	2.5	1.5	0.75	0.75	15.5	25
8/20 Ptet(1)	0.5	2.5	2.5	1.5	0.75	0.75	15.5	25
9/16 Plac 2	0.5	2.5	2.5	1.5	0.75	0.75	15.5	25

PreDenature	Denature	Annealing	Extension	cycle
94　°C	98　°C	57　°C	68　°C	--
2min	10sec	30sec	20sec	30cycles

Yoshida

state	Vector		Inserter		Ligation High ver.2
experiment	9/21 Pcon-RBS-GFP-DT E+X	47.5 µg/mL	9/15 Pcon-RBS-tetR-DT E+S	59 µg/mL	2.3 µg/mL
experiment	9/21 Pcon-RBS-GFP-DT E+X	47.5 µg/mL	9/15 Pcon-apt12_1R-DT E+S	17 µg/mL	2.4 µg/mL
experiment	9/21 Pcon-RBS-GFRDT E+X	2.1 µg/mL	9/15 Pcon-DT181 atte-DT	21 µg/mL	3.0 µg/mL
experiment	9/22 apt 12_1R-DT E+X	67.8 µg/mL	9/18 Pcon-DT181 atte E+S	5.7 µg/mL	3.5 µg/mL
experiment	9/22RBS-GFP-DT E+X	87.7 µg/mL	9/18 Pcon-DT18 atte E+S	6.7 µg/mL	3.5 µg/mL
experiment	9/13 DT E+X	25.4 µg/mL	9/22 Pcon-DT181 anti E+S	46.8 µg/mL	2.3 µg/mL

No name

genome DNA	KOD plus	10x buffer	dNTP	MgSO4	SasA_fwd primer	SasA_rev primer	MilliQ	total
Pcon-atte-DT (E-1A)	0.5	2.5	2.5	1.5	0.75	0.75	77.5	25
Pcon-atte-DT (2-1A)	0.5	2.5	2.5	1.5	0.75	0.75	77.5	25
Pcon-anti-spin-DT (1-2A)	0.5	2.5	2.5	1.5	0.75	0.75	77.5	25
Pcon-anti-spin-DT (E-1A)	0.5	2.5	2.5	1.5	0.75	0.75	77.5	25

PreDenature	Denature	Annealing	Extension	cycle
94　°C	98　°C	57　°C	68　°C	--
2min	10s	30s	45s	30cycle

No name

DNA	concentration[µg/mL]	260/280	260/230
9/22 Plac-DT181 atte-1	204.0	2.00	1.95
9/22 Plac-DT181 atte-2	195.3	1.84	1.62
9/22 Plac-DT181 anti-1	188.2	1.87	2.15
9/22 Plac-DT181 anti-2	165.0	1.95	2.66

Hirano

Lane	Sample	Enzyme1	Enzyme2
1,6	100bp ladder(Promega)
2	Pcon-atte-DT (E-1A)
3	Pcon-atte-DT (2-1A)
4	Pcon-anti-spi-DT (1-2A)
5	Pcon-anti-spi-DT (E-1A)

Hirano

Name	concentration[µg/mL]	260/280	260/230
Pcon atte-DT(E-1A)	166.7	1.51	0.99
Pcon atte-DT(2-1A)	122.1	1.75	1.20
Pcon anti spi DT(1-2A)	99.1	1.86	1.81

@@ Line 125: / Line 125: @@
 <!-- こっから -->
 <div class="experiment">
-<span class="author">じっけんしゃ</span>
+<span class="author">Hirano</span>
 {| class="wikitable"
 !Lane||Sample||Enzyme1||Enzyme2
@@ Line 140: / Line 140: @@
 |}
 [[File:igku_xxxxxx.xxx]]<br>
+</div>
+<!-- ここまでをコピーしてね -->
+===Gel Extraction===
+<!-- こっから -->
+<div class="experiment">
+<span class="author">Hirano</span>
+{| class="wikitable"
+!Name||concentration[&micro;g/mL]||260/280||260/230
+|-
+|Pcon atte-DT(E-1A)||166.7 ||1.51 ||0.99
+|-
+|Pcon atte-DT(2-1A)||122.1 ||1.75 ||1.20
+|-
+|Pcon anti spi DT(1-2A)||99.1 ||1.86 ||1.81
+|}
 </div>
 <!-- ここまでをコピーしてね -->