Template:Kyoto/Notebook/Aug 15

From 2013.igem.org

(Difference between revisions)
(Aug 15)
(Liquid Culture)
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==Aug 15==
==Aug 15==
===Liquid Culture===
===Liquid Culture===
-
<!-- ここから -->
 
<div class="experiment">
<div class="experiment">
-
<span class="author">No name</span>
+
<span class="author">Nakamoto</span>
{| class="wikitable"
{| class="wikitable"
!Sample||medium
!Sample||medium
|-
|-
-
|8/10 BBa_B0034(1)|RBS|  
+
|8/10 BBa_B0034(1)||LB (+Amp)
|-
|-
-
|8/10 BBa_B0034(2) |RBS| 
+
|8/10 BBa_B0034(2)||LB (+Amp)
|-
|-
-
|8/10 BBa_R0011(1)|Plac| 
+
|8/10 BBa_R0011(1)||LB (+CP)
|-
|-
-
|8/10 BBa_R0011(2)|Plac| 
+
|8/10 BBa_R0011(2)||LB (+CP)
|-
|-
-
|8/10 BBa_P0412(1) |LacI| 
+
|8/10 BBa_P0412(1)||LB (+CP)
|-
|-
-
|8/10 BBa_P0412(2) |LacI| 
+
|8/10 BBa_P0412(2)||LB (+CP)
|}
|}
</div>
</div>
-
<!-- ここまでをコピーしてね -->
+
 
===LB Medium Plate===
===LB Medium Plate===
<div class="experiment">
<div class="experiment">

Revision as of 05:42, 24 September 2013

Contents

Aug 15

Liquid Culture

Nakamoto

Samplemedium
8/10 BBa_B0034(1)LB (+Amp)
8/10 BBa_B0034(2)LB (+Amp)
8/10 BBa_R0011(1)LB (+CP)
8/10 BBa_R0011(2)LB (+CP)
8/10 BBa_P0412(1)LB (+CP)
8/10 BBa_P0412(2)LB (+CP)

LB Medium Plate

Nakamoto & Tatsui

volume200ml
Bacto T2ypton 2g
Bacto yeast extract 1g
NaCl1g
0.4µ NaOHaq50µL
Agar Pouder2g 

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
BBa_B0034(1)84.1 2.11 2.01
BBa_B0034(2)86.9 2.03 2.02
BBa_R0011(1)78.3 2.26 2.02
BBa_R0011(2)88.9 2.24 2.02
BBa_P0412(1)15.61 2.10 1.93
BBa_P0412(2)12.93 2.18 1.91