Template:Kyoto/Notebook/Sep 16

From 2013.igem.org

(Difference between revisions)
(Created page with "==Sep 16==")
(→Sep 16)
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==Sep 16==
==Sep 16==
 +
===Liquid Culture===
 +
<div class="experiment">
 +
<span class="author">No name</span>
 +
{| class="wikitable"
 +
!Sample||medium
 +
|}
 +
===Gel Extraction===
 +
<div class="experiment">
 +
<span class="author">じっけんしゃ</span>
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|}
 +
[[File:igku_xxbeforexx.xxx]]<br>
 +
[[File:igku_xxafterxx.xxx]]<br>
 +
{| class="wikitable"
 +
!Name||concentration[&micro;g/mL]||260/280||260/230
 +
|}
 +
===Miniprep===
 +
<div class="experiment">
 +
<span class="author">No name</span>
 +
{|class="wikitable"
 +
!DNA||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|(・▽・)||　||　||
 +
|}
 +
===PCR===
 +
<!-- ここから -->
 +
<div class="experiment">
 +
<span class="author">No name</span>
 +
{| class="wikitable"
 +
!genome DNA||KOD plus||10x buffer||dNTP||MgSO4||SasA_fwd primer||SasA_rev primer||MilliQ||total
 +
|-
 +
| ||0.5||2.5||2.5||1.5||0.75||0.75|| ||25
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|　&deg;C||　&deg;C||　&deg;C||　&deg;C||--
 +
|-
 +
|（◎Д◎）　||　||　||　||
 +
|}
 +
===Electrophoresis===
 +
 +
<!-- こっから -->
 +
<div class="experiment">
 +
<span class="author">じっけんしゃ</span>
 +
{| class="wikitable"
 +
!Lane||Sample||Enzyme1||Enzyme2
 +
|-
 +
|（°Д°）|| || ||
 +
|}
 +
[[File:igku_xxxxxx.xxx]]<br>
 +
===Restriction Enzyme Digestion===
 +
<!-- こっから -->
 +
<div class="experiment">
 +
<span class="author">じっけんしゃ</span>
 +
{| class="wikitable"
 +
! ||DNA||restricion enzyme||restriction enzyme||Buffer||BSA||MilliQ||total
 +
|-
 +
|2 cuts||（°°） &micro;L|| &micro;L|| &micro;L|| &micro;L|| &micro;L|| &micro;L|| &micro;L
 +
|-
 +
|NC|| &micro;L|| &micro;L|| &micro;L|| &micro;L|| &micro;L|| &micro;L|| &micro;L
 +
|}
 +
===Ligation===
 +
 +
<!-- こっから -->
 +
<div class="experiment">
 +
<span class="author">じっけんしゃ</span>
 +
{| class="wikitable"
 +
!state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2
 +
|-
 +
|experiment||name||xx &micro;g/mL||name||xx &micro;g/mL||xx &micro;g/mL
 +
|-
 +
|NC||name||xx &micro;g/mL||name||xx &micro;g/mL||xx &micro;g/mL
 +
|}
 +
===PCR===
 +
<!-- ここから -->
 +
<div class="experiment">
 +
<span class="author">No name</span>
 +
{| class="wikitable"
 +
!genome DNA||KOD plus||10x buffer||dNTP||MgSO4||SasA_fwd primer||SasA_rev primer||MilliQ||total
 +
|-
 +
| ||0.5||2.5||2.5||1.5||0.75||0.75|| ||25
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|　&deg;C||　&deg;C||　&deg;C||　&deg;C||--
 +
|-
 +
|（◎Д◎）　||　||　||　||
 +
|}

Revision as of 05:58, 24 September 2013

Contents

Sep 16

Liquid Culture

No name

Samplemedium

Gel Extraction

じっけんしゃ

LaneDNAEnzyme

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
(・▽・)　　

PCR

No name

genome DNAKOD plus10x bufferdNTPMgSO4SasA_fwd primerSasA_rev primerMilliQtotal
0.52.52.51.50.750.75 25
PreDenatureDenatureAnnealingExtensioncycle
　°C　°C　°C　°C--
（◎Д◎）　　　　

Electrophoresis

じっけんしゃ

LaneSampleEnzyme1Enzyme2
（°Д°）

File:Igku xxxxxx.xxx

Restriction Enzyme Digestion

じっけんしゃ

DNArestricion enzymerestriction enzymeBufferBSAMilliQtotal
2 cuts（°°） µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL

Ligation

じっけんしゃ

stateVectorInserterLigation High ver.2
experimentnamexx µg/mLnamexx µg/mLxx µg/mL
NCnamexx µg/mLnamexx µg/mLxx µg/mL

PCR

No name

genome DNAKOD plus10x bufferdNTPMgSO4SasA_fwd primerSasA_rev primerMilliQtotal
0.52.52.51.50.750.75 25
PreDenatureDenatureAnnealingExtensioncycle
　°C　°C　°C　°C--
（◎Д◎）　　　　
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