Template:Kyoto/Notebook/Sep 5

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==Sep 5==
==Sep 5==
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===Ligation===
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<div class="experiment">
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<span class="author">Kojima</span>
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{| class="wikitable"
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!state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2
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|-
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|experiment||Pconst||13.2||spinach-DT||2.1||3.5
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|-
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|experiment||Plac||15.3||spinach-DT||2.1||3.5
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|-
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|experiment||Pconst||13.2||pT181 antisense (XbaI & PstI)||6.6||3.5
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|-
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|experiment||Plac||15.3||pT181 antisense (XbaI & PstI)||6.6||3.5
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|-
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|experiment||Ptet||30.1||pT181 antisense (XbaI & PstI)||6.6||3.5
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|-
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|experiment||Pconst||13.2||pT181 attenuator||11.7||3.5
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|-
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|experiment||Plac||15.3||pT181 attenuator||11.7||3.5
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|-
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|experiment||Ptet||30.1||RBS-lacZ&alpha;-DT||3.0||3.5
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|-
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|}
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incubate 16 &deg;C 1 hour
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</div>

Revision as of 06:05, 24 September 2013

Sep 5

Ligation

Kojima

stateVectorInserterLigation High ver.2
experimentPconst13.2spinach-DT2.13.5
experimentPlac15.3spinach-DT2.13.5
experimentPconst13.2pT181 antisense (XbaI & PstI)6.63.5
experimentPlac15.3pT181 antisense (XbaI & PstI)6.63.5
experimentPtet30.1pT181 antisense (XbaI & PstI)6.63.5
experimentPconst13.2pT181 attenuator11.73.5
experimentPlac15.3pT181 attenuator11.73.5
experimentPtet30.1RBS-lacZα-DT3.03.5

incubate 16 °C 1 hour

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