Template:Kyoto/Notebook/Sep 20

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==Sep 20==
==Sep 20==
 +
===Ligation===
 +
 +
<!-- こっから -->
 +
<div class="experiment">
 +
<span class="author">Okazaki,Kanaoka,Tatsui,Hirano</span>
 +
{| class="wikitable"
 +
!state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2
 +
|-
 +
|experiment||9/18 Plac(Spe1&Pst1)||3.0&micro;L||9/18 RBS-lysis3-DT (XbaI&PstI)||12.4&micro;L||3.5 &micro;L
 +
|-
 +
|experiment||9/12 Ptet-pT181antisense||1.0&micro;L||9/18 spinach-DT||11.9&micro;L||3.5 &micro;L
 +
|-
 +
|experiment||9/13 Pcon-RBS-luxR-DT||2.1&micro;L||9/10 Plux-RBS-GFP-DT||5.6&micro;L||3.5 &micro;L
 +
|-
 +
|experiment||9/13 Pcon-pT181attenuator(SpeI&PstI)||2.0&micro;L||9/18 aptamer12-1R-DT (Xba1&Pst1)||6.3&micro;L||3.5 &micro;L
 +
|-
 +
|experiment||9/18 Plac2(Spe1&Ps)||3.0&micro;L||9/18 aptamer12-1R-DT(Xba1&Pst1)||4.5&micro;L||x3.5&micro;L
 +
|-
 +
|}
 +
</div>
 +
<!-- ここまでをコピーしてね -->
 +
 +
===Transformation===
 +
<div class="experiment">
 +
<span class="author">Tatsui</span>
 +
{| class="wikitable"
 +
!Name||Sample||Competent Cells||Plate
 +
|-
 +
|9/17 IC3-Pcon-pT181attenuator-DT||2&micro;L||20&micro;L||CP
 +
|-
 +
|9/17 IC3-Pcon-aptamer12-1R-DT||2&micro;L||20&micro;L||CP
 +
|-
 +
|9/17 IC3-Pcon-antisense-spinach-DT||2&micro;L||20&micro;L||CP
 +
|-
 +
|9/17 IC3-Pcon-RBS-tetR-DTx2||2&micro;L||20&micro;L||CP
 +
|-
 +
|9/14 IC3-PkaiBC||2&micro;L||20&micro;L||CP
 +
|-
 +
|9/14 IC3-RpaB||2&micro;L||20&micro;L||CP
 +
|-
 +
|9/16 IC3-pT181attenuator(EcoRI&SpeI) ||2&micro;L||20&micro;L||CP
 +
|-
 +
|9/17 IC3-pT181attenuator(XbaI&PstI) ||2&micro;L||20&micro;L||CP
 +
|-
 +
|9/17IC3-Pcon-spinach-DT||2&micro;L||20&micro;L||CP
 +
|-
 +
|9/14 RBS-GFP-DT-PkaiBC||2&micro;L||20&micro;L||CP
 +
|-
 +
|Plux-RBS-lysis3-DT||2&micro;L||20&micro;L||CP
 +
|-
 +
|9/20 Ptet-pT181antisense-spinach-DT||2&micro;L||20&micro;L||CP
 +
|-
 +
|9/20 Pcon-spinach-DT(4K5) ||2&micro;L||20&micro;L||K
 +
|-
 +
|9/20 Pcon-pT181antisense-spinach-DT(4K5) ||2&micro;L||20&micro;L||K
 +
|-
 +
|9/20 KaiABC||2&micro;L||20&micro;L||K
 +
|-
 +
|9/20 KaiABC||2&micro;L||20&micro;L||K
 +
|-
 +
|9/20 Plac-RBS-lysis3-DT||2&micro;L||20&micro;L||Amp
 +
|-
 +
|9/20 Plac-aptamer12-1R||2&micro;L||20&micro;L||Amp
 +
|-
 +
|9/20 Plux-RBS-GFP-DT-Pcon-RBS-luxR-DT||2&micro;L||20&micro;L||Amp
 +
|-
 +
|Plac-aptamer12-1R-DT||2&micro;L||20&micro;L||Amp
 +
|-
 +
|9/20 Plac-aptamer12-1R-DT||2&micro;L||20&micro;L||Amp
 +
|-
 +
|9/20Pcon-pT181attenuator-aptamer-DT||2&micro;L||20&micro;L||Amp
 +
|-
 +
|9/17 Plac-pT181-attenuatorx2||2&micro;L||20&micro;L||Amp
 +
|-
 +
|9/15 Pcon-RBS-lacZ&alpha;-DT||2&micro;L||20&micro;L||Amp
 +
|-
 +
|9/14 Pcon-pT181attenuator-RBS-lacZ&alpha;-DT||2&micro;L||20&micro;L||Amp
 +
|-
 +
|PUC19||2&micro;L||20&micro;L||Amp
 +
|-
 +
 +
|}
 +
</div>
 +
 +
===PCR===
 +
<!-- ここから -->
 +
<div class="experiment">
 +
<span class="author">Kojima and Yoshida</span>
 +
{| class="wikitable"
 +
!Sample||base pair
 +
|-
 +
|pT181attenuator(IC3)(XbaI&PstI)||--
 +
|-
 +
|Pcon-RBS-lacZ&alpha;-DT||--
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
 +
|-
 +
|5min||30s||30s||3min||30cycles
 +
|}
 +
</div>
 +
===Electrophoresis===
 +
<div class="experiment">
 +
<span class="author">No name</span>
 +
{| class="wikitable"
 +
!Lane||Sample||Enzyme
 +
|-
 +
|1||Pcon-RBS-lacZ&alpha;-DT||--
 +
|-
 +
|2|| 1kb ladder||--
 +
|-
 +
|3||IC3-pT181attenuator ||--
 +
|}
 +
</div>

Revision as of 08:38, 24 September 2013

Contents

Sep 20

Ligation

Okazaki,Kanaoka,Tatsui,Hirano

stateVectorInserterLigation High ver.2
experiment9/18 Plac(Spe1&Pst1)3.0µL9/18 RBS-lysis3-DT (XbaI&PstI)12.4µL3.5 µL
experiment9/12 Ptet-pT181antisense1.0µL9/18 spinach-DT11.9µL3.5 µL
experiment9/13 Pcon-RBS-luxR-DT2.1µL9/10 Plux-RBS-GFP-DT5.6µL3.5 µL
experiment9/13 Pcon-pT181attenuator(SpeI&PstI)2.0µL9/18 aptamer12-1R-DT (Xba1&Pst1)6.3µL3.5 µL
experiment9/18 Plac2(Spe1&Ps)3.0µL9/18 aptamer12-1R-DT(Xba1&Pst1)4.5µLx3.5µL

Transformation

Tatsui

NameSampleCompetent CellsPlate
9/17 IC3-Pcon-pT181attenuator-DT2µL20µLCP
9/17 IC3-Pcon-aptamer12-1R-DT2µL20µLCP
9/17 IC3-Pcon-antisense-spinach-DT2µL20µLCP
9/17 IC3-Pcon-RBS-tetR-DTx22µL20µLCP
9/14 IC3-PkaiBC2µL20µLCP
9/14 IC3-RpaB2µL20µLCP
9/16 IC3-pT181attenuator(EcoRI&SpeI) 2µL20µLCP
9/17 IC3-pT181attenuator(XbaI&PstI) 2µL20µLCP
9/17IC3-Pcon-spinach-DT2µL20µLCP
9/14 RBS-GFP-DT-PkaiBC2µL20µLCP
Plux-RBS-lysis3-DT2µL20µLCP
9/20 Ptet-pT181antisense-spinach-DT2µL20µLCP
9/20 Pcon-spinach-DT(4K5) 2µL20µLK
9/20 Pcon-pT181antisense-spinach-DT(4K5) 2µL20µLK
9/20 KaiABC2µL20µLK
9/20 KaiABC2µL20µLK
9/20 Plac-RBS-lysis3-DT2µL20µLAmp
9/20 Plac-aptamer12-1R2µL20µLAmp
9/20 Plux-RBS-GFP-DT-Pcon-RBS-luxR-DT2µL20µLAmp
Plac-aptamer12-1R-DT2µL20µLAmp
9/20 Plac-aptamer12-1R-DT2µL20µLAmp
9/20Pcon-pT181attenuator-aptamer-DT2µL20µLAmp
9/17 Plac-pT181-attenuatorx22µL20µLAmp
9/15 Pcon-RBS-lacZα-DT2µL20µLAmp
9/14 Pcon-pT181attenuator-RBS-lacZα-DT2µL20µLAmp
PUC192µL20µLAmp

PCR

Kojima and Yoshida

Samplebase pair
pT181attenuator(IC3)(XbaI&PstI)--
Pcon-RBS-lacZα-DT--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s3min30cycles

Electrophoresis

No name

LaneSampleEnzyme
1Pcon-RBS-lacZα-DT--
2 1kb ladder--
3IC3-pT181attenuator --
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