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Template:Kyoto/Notebook/Sep 20
From 2013.igem.org
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!state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2 | !state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2 | ||
|- | |- | ||
| - | |experiment||9/18 Plac( | + | |experiment||9/18 Plac(SpeI&PstI)||3.0µL||9/18 RBS-lysis3-DT (XbaI&PstI)||12.4µL||3.5 µL |
|- | |- | ||
|experiment||9/12 Ptet-pT181antisense||1.0µL||9/18 spinach-DT||11.9µL||3.5 µL | |experiment||9/12 Ptet-pT181antisense||1.0µL||9/18 spinach-DT||11.9µL||3.5 µL | ||
| Line 14: | Line 14: | ||
|experiment||9/13 Pcon-RBS-luxR-DT||2.1µL||9/10 Plux-RBS-GFP-DT||5.6µL||3.5 µL | |experiment||9/13 Pcon-RBS-luxR-DT||2.1µL||9/10 Plux-RBS-GFP-DT||5.6µL||3.5 µL | ||
|- | |- | ||
| - | |experiment||9/13 Pcon-pT181attenuator(SpeI&PstI)||2.0µL||9/18 aptamer12-1R-DT ( | + | |experiment||9/13 Pcon-pT181attenuator(SpeI&PstI)||2.0µL||9/18 aptamer12-1R-DT (XbaI&PstI)||6.3µL||3.5 µL |
|- | |- | ||
| - | |experiment||9/18 Plac2(Spe1& | + | |experiment||9/18 Plac2(Spe1&PstI)||3.0µL||9/18 aptamer12-1R-DT(XbaI&PstI)||4.5µL||x3.5µL |
|- | |- | ||
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Revision as of 08:40, 24 September 2013
Contents |
Sep 20
Ligation
| state | Vector | Inserter | Ligation High ver.2 | ||
|---|---|---|---|---|---|
| experiment | 9/18 Plac(SpeI&PstI) | 3.0µL | 9/18 RBS-lysis3-DT (XbaI&PstI) | 12.4µL | 3.5 µL |
| experiment | 9/12 Ptet-pT181antisense | 1.0µL | 9/18 spinach-DT | 11.9µL | 3.5 µL |
| experiment | 9/13 Pcon-RBS-luxR-DT | 2.1µL | 9/10 Plux-RBS-GFP-DT | 5.6µL | 3.5 µL |
| experiment | 9/13 Pcon-pT181attenuator(SpeI&PstI) | 2.0µL | 9/18 aptamer12-1R-DT (XbaI&PstI) | 6.3µL | 3.5 µL |
| experiment | 9/18 Plac2(Spe1&PstI) | 3.0µL | 9/18 aptamer12-1R-DT(XbaI&PstI) | 4.5µL | x3.5µL |
Transformation
| Name | Sample | Competent Cells | Plate |
|---|---|---|---|
| 9/17 IC3-Pcon-pT181attenuator-DT | 2µL | 20µL | CP |
| 9/17 IC3-Pcon-aptamer12-1R-DT | 2µL | 20µL | CP |
| 9/17 IC3-Pcon-antisense-spinach-DT | 2µL | 20µL | CP |
| 9/17 IC3-Pcon-RBS-tetR-DTx2 | 2µL | 20µL | CP |
| 9/14 IC3-PkaiBC | 2µL | 20µL | CP |
| 9/14 IC3-RpaB | 2µL | 20µL | CP |
| 9/16 IC3-pT181attenuator(EcoRI&SpeI) | 2µL | 20µL | CP |
| 9/17 IC3-pT181attenuator(XbaI&PstI) | 2µL | 20µL | CP |
| 9/17IC3-Pcon-spinach-DT | 2µL | 20µL | CP |
| 9/14 RBS-GFP-DT-PkaiBC | 2µL | 20µL | CP |
| Plux-RBS-lysis3-DT | 2µL | 20µL | CP |
| 9/20 Ptet-pT181antisense-spinach-DT | 2µL | 20µL | CP |
| 9/20 Pcon-spinach-DT(4K5) | 2µL | 20µL | K |
| 9/20 Pcon-pT181antisense-spinach-DT(4K5) | 2µL | 20µL | K |
| 9/20 KaiABC | 2µL | 20µL | K |
| 9/20 KaiABC | 2µL | 20µL | K |
| 9/20 Plac-RBS-lysis3-DT | 2µL | 20µL | Amp |
| 9/20 Plac-aptamer12-1R | 2µL | 20µL | Amp |
| 9/20 Plux-RBS-GFP-DT-Pcon-RBS-luxR-DT | 2µL | 20µL | Amp |
| Plac-aptamer12-1R-DT | 2µL | 20µL | Amp |
| 9/20 Plac-aptamer12-1R-DT | 2µL | 20µL | Amp |
| 9/20Pcon-pT181attenuator-aptamer-DT | 2µL | 20µL | Amp |
| 9/17 Plac-pT181-attenuatorx2 | 2µL | 20µL | Amp |
| 9/15 Pcon-RBS-lacZα-DT | 2µL | 20µL | Amp |
| 9/14 Pcon-pT181attenuator-RBS-lacZα-DT | 2µL | 20µL | Amp |
| PUC19 | 2µL | 20µL | Amp |
PCR
| Sample | base pair |
|---|---|
| pT181attenuator(IC3)(XbaI&PstI) | -- |
| Pcon-RBS-lacZα-DT | -- |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94°C | 94°C | 55°C | 68°C | -- |
| 5min | 30s | 30s | 3min | 30cycles |
Electrophoresis
| Lane | Sample | Enzyme |
|---|---|---|
| 1 | Pcon-RBS-lacZα-DT | -- |
| 2 | 1kb ladder | -- |
| 3 | IC3-pT181attenuator | -- |
