Template:Kyoto/Notebook/Sep 11

From 2013.igem.org

(Difference between revisions)
(→Sep 11)
(→colony PCR)
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|9/9Pcon-spinach-DT(p3B4k5)||605bp
|9/9Pcon-spinach-DT(p3B4k5)||605bp
|}
|}
-
{| class="wikitable"
 
-
!PreDenature||Denature||Annealing||Extension||cycle
 
-
|-
 
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|　94°C||　94°C||　55°C||　68°C||--
 
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|-
 
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|5min||30s　||30s　||42s　||
 
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|}
 
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</div>
 
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<div>
 
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{| class="wikitable"
 
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!Sample||base pair
 
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|-
 
-
 
{| class="wikitable"
{| class="wikitable"
!PreDenature||Denature||Annealing||Extension||cycle
!PreDenature||Denature||Annealing||Extension||cycle
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|-
|-
|9/9Pcon+RBS-luxI-DT||1079bp
|9/9Pcon+RBS-luxI-DT||1079bp
 +
|-
 +
|9/10Pcon+sptamer12_1R-DT||584bp
 +
|-
 +
|9/10DT+Pcon-pT181 attenuater 1||781bp
 +
|-
 +
|9/10Fusion1 attenuater(p3B1C3)-1||638bp
|}
|}
{| class="wikitable"
{| class="wikitable"
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!Sample||base pair
!Sample||base pair
|-
|-
-
|9/10Pcon+sptamer12_1R-DT||584bp
+
||}
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|}
+
{| class="wikitable"
{| class="wikitable"
!PreDenature||Denature||Annealing||Extension||cycle
!PreDenature||Denature||Annealing||Extension||cycle
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!Sample||base pair
!Sample||base pair
|-
|-
-
|9/10DT+Pcon-pT181 attenuater 1||781bp
+
 
|}
|}
{| class="wikitable"
{| class="wikitable"
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!Sample||base pair
!Sample||base pair
|-
|-
-
|9/10Fusion1 attenuater(p3B1C3)-1||638bp
 
|}
|}
{| class="wikitable"
{| class="wikitable"

Revision as of 10:34, 24 September 2013

Sep 11

PCR

Tatsui

pKaiBC(200p)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
0.52.52.51.51.50.516 25
PreDenatureDenatureAnnealingExtensioncycle
　94°C　98°C　60°C　68°C30
2min　10sec　30sec　30sec　
pKaiBC(400p)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
12.52.51.51.50.515.5 25
PreDenatureDenatureAnnealingExtensioncycle
　94°C　98°C　60°C　68°C30
2min　10sec　30sec　30sec　
pKaiBC(800p)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
22.52.51.51.50.514.5 25
PreDenatureDenatureAnnealingExtensioncycle
　94°C　98°C　60°C　68°C30
2min　10sec　30sec　30sec　
pKaiBC(1ng)10*buffer KOD plusdNTPsMgSO4primer mix(F&R)KOD plusMilliQtotal
2.52.52.51.51.50.514 25
PreDenatureDenatureAnnealingExtensioncycle
　94°C　98°C　60°C　68°C30
2min　10sec　30sec　30sec　

colony PCR

Tatsui

Samplebase pair
9/9Pcon-lacZα(p3B4k5)712bp
9/9Pcon-spinach-DT(p3B4k5)605bp
PreDenatureDenatureAnnealingExtensioncycle
　94°C　94°C　55°C　68°C--
5min30s　30s　42s　
Samplebase pair
Pbad\araC-RBS-luxI-DT
PreDenatureDenatureAnnealingExtensioncycle
　94°C　94°C　55°C　68°C--
5min30s　30s　2min　
Samplebase pair
9/9Pcon+RBS-luxI-DT1079bp
9/10Pcon+sptamer12_1R-DT584bp
9/10DT+Pcon-pT181 attenuater 1781bp
9/10Fusion1 attenuater(p3B1C3)-1638bp
PreDenatureDenatureAnnealingExtensioncycle
　94°C　94°C　55°C　68°C--
50sec　30sec　30sec　1min　
Samplebase pair
}
PreDenatureDenatureAnnealingExtensioncycle
　94°C　94°C　55°C　68°C--
50sec　30sec　30sec　1min　
Samplebase pair
PreDenatureDenatureAnnealingExtensioncycle
　94°C　94°C　55°C　68°C--
50sec　30sec　30sec　1min　
Samplebase pair
PreDenatureDenatureAnnealingExtensioncycle
　94°C　94°C　55°C　68°C--
50sec　30sec　30sec　1min　
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