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Template:Kyoto/Notebook/Sep 7
From 2013.igem.org
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|apt12_1R(pSB1C3)||230.0 ||1.98 ||2.28 | |apt12_1R(pSB1C3)||230.0 ||1.98 ||2.28 | ||
| + | |} | ||
| + | </div> | ||
| + | <!-- ここまでをコピーしてね --> | ||
| + | ===Ligation=== | ||
| + | |||
| + | <!-- こっから --> | ||
| + | <div class="experiment"> | ||
| + | <span class="author">No Name</span> | ||
| + | {| class="wikitable" | ||
| + | !state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2 | ||
| + | |- | ||
| + | |experiment||9/6 Plux (SpeI&PstI)||2 µL||9/7 RBS-lysis2-DT (EcoRI&XbaI)|| µL||3.5 µL | ||
| + | |- | ||
| + | |experiment||9/6 Plac (SpeI&PstI)|| µL||9/7 RBS-lysis2-DT (EcoRI&XbaI)|| µL||3.5 µL | ||
| + | |- | ||
| + | |experiment||9/6 Pcon-RBS-luxR-DT (EcoRI&XbaI)||1.9 µL||9/6 Plux-RBS-GFP-DT(SpeI&PstI)||2.2 µL||3.5 µL | ||
| + | |- | ||
| + | |experiment||9/6 Pbed/araC (SpeI&PstI)||1.6 µL||9/5 RBS-luxI-DT (EcoRI&XbaI)||16 µL||3.5 µL | ||
| + | |- | ||
| + | |experiment||9/6 Plac (SpeI&PstI)||1.5 µL||9/5 pT181 antisense (EcoRI&XbaI)||2.0 µL||3.5 µL | ||
| + | |- | ||
| + | |experiment||9/6 Pcon (SpeI&PstI)||3.0 µL||9/5 RBS-luxI-DT (EcoRI&XbaI)||10 µL||3.5 µL | ||
|} | |} | ||
</div> | </div> | ||
<!-- ここまでをコピーしてね --> | <!-- ここまでをコピーしてね --> | ||
Revision as of 12:11, 24 September 2013
Contents |
Sep 7
Gel Extraction
| Name | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| apt 12_1R-DT | 12.7 | 1.62 | 5.74 |
| RBS-lysis2-DT | 6.7 | 1.71 | 5.46 |
| Pλ-luvI | 8.1 | 1.76 | 2.25 |
Miniprep
| DNA | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| RBS-lysis1-DT | 195.3 | ||
| RBS-lysis3-DT | 255.5 | 1.91 | 2.04 |
| Ptet-pT18 | 183.6 | 1.85 | 1.48 |
| Pcon-RBS-tetR-DT | 134.3 | 1.94 | 2.20 |
| Ptet-RBS-lacZα-DT | 67.7 | 1.92 | 1.79 |
| Pcon-pT181 attenuator | 232.3 | 1.95 | 1.90 |
| pSB4K5 | 237.0 | 1.61 | 1.50 |
| pT181 antisense(pSB1C3) | 256.4 | 1.63 | 1.78 |
| apt12_1R(pSB1C3) | 230.0 | 1.98 | 2.28 |
Ligation
| state | Vector | Inserter | Ligation High ver.2 | ||
|---|---|---|---|---|---|
| experiment | 9/6 Plux (SpeI&PstI) | 2 µL | 9/7 RBS-lysis2-DT (EcoRI&XbaI) | µL | 3.5 µL |
| experiment | 9/6 Plac (SpeI&PstI) | µL | 9/7 RBS-lysis2-DT (EcoRI&XbaI) | µL | 3.5 µL |
| experiment | 9/6 Pcon-RBS-luxR-DT (EcoRI&XbaI) | 1.9 µL | 9/6 Plux-RBS-GFP-DT(SpeI&PstI) | 2.2 µL | 3.5 µL |
| experiment | 9/6 Pbed/araC (SpeI&PstI) | 1.6 µL | 9/5 RBS-luxI-DT (EcoRI&XbaI) | 16 µL | 3.5 µL |
| experiment | 9/6 Plac (SpeI&PstI) | 1.5 µL | 9/5 pT181 antisense (EcoRI&XbaI) | 2.0 µL | 3.5 µL |
| experiment | 9/6 Pcon (SpeI&PstI) | 3.0 µL | 9/5 RBS-luxI-DT (EcoRI&XbaI) | 10 µL | 3.5 µL |
