Template:Kyoto/Notebook/Sep 10

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==Sep 10==
==Sep 10==
 +
===Ligation===
 +
 +
<!-- こっから -->
 +
<div class="experiment">
 +
<span class="author">Nakamoto</span>
 +
{| class="wikitable"
 +
!state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2
 +
|-
 +
|experiment||9/6 Pcon-RBS-luxR-DT||1.9&micro;L||9/6Plux-RBS-GFP-DT||22 &micro;L|| &micro;L
 +
|-
 +
|experiment||9/6 Plux||2&micro;L||9/8 RBS-lysis1-DT||6.6&micro;L||xx &micro;L
 +
|-
 +
|experiment||9/6 Plux||2&micro;L||9/8 RBS-lysis2-DT||6.3&micro;L||xx &micro;L
 +
|-
 +
|experiment||9/8 Ptet-PT181 antisense||1.6&micro;L||9/8 spinach-DT||4.4&micro;L||xx &micro;L
 +
|-
 +
|experiment||9/8 Pcon-PT181 attenuator||3.4&micro;L||9/8 apz 12-1R-PT||2.1&micro;L||xx &micro;L
 +
|-
 +
|experiment||9/8 Pcon||3.4&micro;L||9/8 apz 12-1R-PT||2.3&micro;L||xx &micro;L
 +
|-
 +
|experiment||9/8 DT||3.0&micro;L||9/8 Pcon-PT181 attenuator||7.9&micro;L||xx &micro;L
 +
|-
 +
|experiment||9/10 pSB1C3||1.8&micro;L||9/8 F3m2 attenuator||4.6&micro;L||xx &micro;L
 +
|-
 +
|experiment||9/10 pSB1C3||1.8&micro;L||9/8 F1 attenuator||3.4&micro;L||xx &micro;L
 +
|}
 +
</div>
 +
<!-- ここまでをコピーしてね -->
 +
 +
===Miniprep===
 +
<div class="experiment">
 +
<span class="author">Nakamoto</span>
 +
{|class="wikitable"
 +
!DNA||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|spinach-DT||202.4||1.97||1.93
 +
|-
 +
|Plux-RBS-GFP-DT||164.2||2.80||2.06
 +
|-
 +
|Pcon-DT attenuator|205.4||1.81||2.39
 +
|-
 +
|RSBJC3-2||240.8||1.73||1.43
 +
|-
 +
|Plaz-1||240.8||1.73||1.43
 +
|}
 +
</div>
 +
<!-- ここまでをコピーしてね -->
 +
 +
===Restriction Enzyme Digestion===
 +
<!-- こっから -->
 +
<div class="experiment">
 +
<span class="author">Nakamoto</span>
 +
{| class="wikitable"
 +
! ||9/10　Plux-RBS-GFP-DT||EcoR1||Spel||Buffer||BSA||MilliQ||total
 +
|-
 +
|2cuts||1&micro;L||1&micro;L||3&micro;L||3&micro;L||9.9&micro;L||30&micro;L
 +
|-
 +
|NC|| 0&micro;L||0&micro;L||1&micro;L||1&micro;L||7.4&micro;L||10&micro;L
 +
|}
 +
{| class="wikitable"
 +
! ||9/10　Spinach-DT||Xbal||Pstl||Buffer||BSA||MilliQ||total
 +
|-
 +
|2cuts||1&micro;L||1&micro;L||3&micro;L||3&micro;L||12.1&micro;L||30&micro;L
 +
|-
 +
|NC|| 0&micro;L||0&micro;L||1&micro;L||1&micro;L||7.5&micro;L||10&micro;L
 +
|}
 +
{| class="wikitable"
 +
! ||9/6 apt12-1R -DT||Xbal||Pstl||Buffer||BSA||MilliQ||total
 +
|-
 +
|2cuts||1&micro;L||1&micro;L||3&micro;L||3&micro;L||14.0&micro;L||30&micro;L
 +
|-
 +
|NC|| 0&micro;L||0&micro;L||1&micro;L||1&micro;L||7.6&micro;L||10&micro;L
 +
|}
 +
{| class="wikitable"
 +
! ||9/10　Pcon-pt81 attenuator ||EcoR1||Spel||Buffer||BSA||MilliQ||total
 +
|-
 +
|2cuts||1&micro;L||1&micro;L||3&micro;L||3&micro;L||12.2&micro;L||30&micro;L
 +
|-
 +
|NC|| 0&micro;L||0&micro;L||1&micro;L||1&micro;L||7.5&micro;L||10&micro;L
 +
|}
 +
</div>
 +
<!-- ここまでをコピーしてね -->
 +
 +
===Electrophoresis===
 +
 +
<!-- こっから -->
 +
<div class="experiment">
 +
<span class="author">じっけんしゃ</span>
 +
{| class="wikitable"
 +
!Lane||Sample||Enzyme1||Enzyme2
 +
|-
 +
|1||100bp ladder||--||--
 +
|-
 +
|2|| Plux-RBS-GEP-DT||EcoR1||Spel
 +
|-
 +
|3|| Plux-RBS-GEP-DT||--||--
 +
|-
 +
|4||Spinach-DT||Xbal||Pstl
 +
|-
 +
|5||Spinach-DT||--||--
 +
|-
 +
|6||aptamer 121R-DT||Xbal||Pstl
 +
|-
 +
|7||aptamer 121R-DT||--||--
 +
|-
 +
|8||Pcon-pt181 attenuator|||EcoR1||Spel
 +
|-
 +
|9|| Pcon-pt181 attenuator||--||--
 +
|-
 +
|10||100bp ladder||--||--
 +
|}
 +
[[File:igku_xxxxxx.xxx]]<br>
 +
</div>
 +
<!-- ここまでをコピーしてね -->
 +
 +
 +
===Colony PCR===
 +
<!-- ここから -->
 +
<div class="experiment">
 +
<span class="author">Tatsui</span>
 +
{| class="wikitable"
 +
!Sample||base pair
 +
|-
 +
|9/9 Pcon-pT181 antisense||--
 +
|-
 +
|9/8 J223100(entA)||--
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
 +
|-
 +
|5min||30s||30s||1min10sec||
 +
|}
 +
</div>
 +
<!-- ここまでをコピーしてね -->
 +
 +
===Electrophoresis===
 +
 +
<!-- こっから -->
 +
<div class="experiment">
 +
<span class="author">Tatsui</span>
 +
{| class="wikitable"
 +
!Lane||Sample
 +
|-
 +
|1||100bp ladder
 +
|-
 +
|2||Pcon Pt181 antisense1+spinach-DT
 +
|-
 +
|3||Pcon Pt181 antisense2+spinach-DT
 +
|-
 +
|4||Pcon Pt181 antisense3+spinach-DT
 +
|-
 +
|5||Pcon Pt181 antisense4+spinach-DT
 +
|-
 +
|6||Pcon Pt181 antisense5+spinach-DT
 +
|-
 +
|7||Pcon Pt181 antisense6+spinach-DT
 +
|-
 +
|8||Pcon Pt181 antisense7+spinach-DT
 +
|-
 +
|9||Pcon Pt181 antisense8+spinach-DT
 +
|-
 +
|10||Pcon Pt181 antisense9+spinach-DT
 +
|-
 +
|11||Pcon Pt181 antisense10+spinach-DT
 +
|-
 +
|12||Pcon Pt181 antisense11+spinach-DT
 +
|-
 +
|13||Pcon Pt181 antisense12+spinach-DT
 +
|-
 +
|14||100bp ladder
 +
|}
 +
[[File:igku_xxxxxx.xxx]]<br>
 +
{| class="wikitable"
 +
!Lane||Sample
 +
|-
 +
|1||1kbp ladder
 +
|-
 +
|2||Pcon entA1
 +
|-
 +
|3||Pcon entA2
 +
|-
 +
|4||Pcon entA3
 +
|-
 +
|5||1kbp ladder
 +
|}
 +
</div>
 +
<!-- ここまでをコピーしてね -->
 +
 +
===Gel Extraction===
 +
<!-- こっから -->
 +
<div class="experiment">
 +
<span class="author">Tatsui</span>
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||1kbp ladder||--
 +
|-
 +
|2||KaiA(200pg)||--
 +
|-
 +
|3||KaiA(200pg)||--
 +
|-
 +
|4||--||--
 +
|-
 +
|5||KaiA(400pg)||--
 +
|-
 +
|6||KaiA(400pg)||--
 +
|-
 +
|7||--||--
 +
|-
 +
|8||KaiA(800pg||--
 +
|-
 +
|9||KaiA(800pg)||--
 +
|-
 +
|10||1kbp ladder||--
 +
|}
 +
[[File:igku_xxbeforexx.xxx]]<br>
 +
[[File:igku_xxafterxx.xxx]]<br>
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||1kbp ladder||--
 +
|-
 +
|2||PKaiBC(200pg)||--
 +
|-
 +
|3||PKaiBC(200pg)||--
 +
|-
 +
|4||--||--
 +
|-
 +
|5||PKaiBC(400pg)||--
 +
|-
 +
|6||PKaiBC(400pg)||--
 +
|-
 +
|7||--||--
 +
|-
 +
|8||PKaiBC(800pg||--
 +
|-
 +
|9||PKaiBC(800pg)||--
 +
|-
 +
|10||1kbp ladder||--
 +
|}
 +
</div>
 +
<!-- ここまでをコピーしてね -->
 +
 +
===Liquid Culture===
 +
<!-- ここから -->
 +
<div class="experiment">
 +
<span class="author">Hirano</span>
 +
{| class="wikitable"
 +
!Sample||medium
 +
|-
 +
|9/4 J23100&#9312;(JM109)||LB(Amp)
 +
|-
 +
|9/8 J23100&#9312; (entA)||LB(Amp)　
 +
|}
 +
</div>
 +
<!-- ここまでをコピーしてね -->
 +
 +
===Gel Extraction===
 +
<!-- こっから -->
 +
<div class="experiment">
 +
<span class="author">Tatsui</span>
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||1kbp ladder||--
 +
|-
 +
|2||KaiC||--
 +
|-
 +
|3||KaiC||--
 +
|-
 +
|4||KaiC||--
 +
|-
 +
|5||--||--
 +
|-
 +
|6||1kbp ladder||--
 +
|-
 +
|7||1kbp ladder||--
 +
|-
 +
|8||KaiC||--
 +
|-
 +
|9||KaiC||--
 +
|-
 +
|10||KaiC||--
 +
|11||--||--
 +
|-
 +
|12||1kbp ladder||--
 +
|}
 +
[[File:igku_xxbeforexx.xxx]]<br>
 +
[[File:igku_xxafterxx.xxx]]<br>
 +
{| class="wikitable"
 +
!Name||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|（´ω｀）|| || ||
 +
|-
 +
|（ΦωΦ^）|| || ||
 +
|}
 +
</div>
 +
<!-- ここまでをコピーしてね -->
 +
 +
===Liquid Culture===
 +
<!-- ここから -->
 +
<div class="experiment">
 +
<span class="author">Tatsui</span>
 +
{| class="wikitable"
 +
!Sample||medium
 +
|-
 +
|9/10 Pcon-pt181 antisense-Spinach-DT||Plusgrow 　
 +
|}
 +
</div>
 +
<!-- ここまでをコピーしてね -->
 +
 +
===Colony PCR===
 +
<!-- ここから -->
 +
<div class="experiment">
 +
<span class="author">No name</span>
 +
{| class="wikitable"
 +
!Sample||base pair
 +
|-
 +
|9/9 Phad larac-1 RBS-lux1-DT||2331
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
 +
|-
 +
|5min||30s||30s||2min||
 +
|}
 +
{| class="wikitable"
 +
!Sample||base pair
 +
|-
 +
|9/9 Pcon-lacZ&alpha; (psB4KS)||712
 +
|-
 +
|9/9 Pcon-Spinach-DT(psB4KS)||605
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
 +
|-
 +
|5min||30s||30s||42s||
 +
|}
 +
{| class="wikitable"
 +
!Sample||base pair
 +
|-
 +
|9/9 Plac+RBS-lysis3}-DT||1273
 +
|-
 +
|9/9 Pcon+RBS-lux1-DT||1079
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
 +
|-
 +
|5min||30s||30s||1min12s||
 +
|}
 +
 +
</div>
 +
<!-- ここまでをコピーしてね -->
 +
 +
===Electrophoresis===
 +
 +
<!-- こっから -->
 +
<div class="experiment">
 +
<span class="author">No name</span>
 +
{| class="wikitable"
 +
!Lane||Sample||Enzyme1||Enzyme2
 +
|-
 +
|1||100bp ladder||--||--
 +
|-
 +
|2||9/9 Pcou-RBS-lacZ&alpha;-DT(p3B4KS9)-1||--||--
 +
|-
 +
|3||9/9 Pcou-RBS-lacZ&alpha;-DT(p3B4KS9)-2||--||--
 +
|-
 +
|4||9/9 Pcou-Spinach-DT(pB4KS)-1||--||--
 +
|-
 +
|5||9/9 Pcou-Spinach-DT(pB4KS)-2||--||--
 +
|-
 +
|6||100bp ladder||--||--
 +
|}
 +
[[File:igku_xxxxxx.xxx]]<br>
 +
</div>
 +
<!-- ここまでをコピーしてね -->
 +
===Transformation===
 +
<div class="experiment">
 +
<span class="author">Nakamoto</span>
 +
{| class="wikitable"
 +
!Name||Sample||Competent Cells||Total||Plate
 +
|-
 +
|F1 attenuator +p3B1C3||&micro;L||&micro;L||&micro;L||CP
 +
|-
 +
|F3n2 attenuator +p3B1C3||&micro;L||&micro;L||&micro;L||CP
 +
|-
 +
|F1 antisense +p3B1C3||&micro;L||&micro;L||&micro;L||CP
 +
|-
 +
|F6 antisense+p3B1C3||&micro;L||&micro;L||&micro;L||CP
 +
|-
 +
|apt12-P +p3B1C3||&micro;L||&micro;L||&micro;L||CP
 +
|-
 +
|apt12-1M +p3B1C3||&micro;L||&micro;L||&micro;L||CP
 +
|-
 +
|Pcon-luxR+Plux-GFP||&micro;L||&micro;L||&micro;L||Amp
 +
|-
 +
|Plux+RBS-lysis1-DT||&micro;L||&micro;L||&micro;L||CP
 +
|-
 +
|Plux+RBS-lysis2-DT||&micro;L||&micro;L||&micro;L||CP
 +
|-
 +
|Ptet-pT181antisense+Spinach-DT||&micro;L||&micro;L||&micro;L||CP
 +
|-
 +
|Pcon-pT181 attenuator+apt121R-DT||&micro;L||&micro;L||&micro;L||Amp
 +
|-
 +
|Pcon-1 apt12-1R-DT||&micro;L||&micro;L||&micro;L||Amp
 +
|-
 +
|DT+Pcon-pT181 attenuator||&micro;L||&micro;L||&micro;L||CP
 +
|}
 +
</div>
 +
<!-- ここまでをコピーしてね -->
 +
 +
===Electrophoresis===
 +
 +
<!-- こっから -->
 +
<div class="experiment">
 +
<span class="author">Okazaki</span>
 +
{| class="wikitable"
 +
!Lane||Sample||Enzyme1||Enzyme2
 +
|-
 +
|1||100bp ladder||--||--
 +
|-
 +
|2||Spinach-DT||Xbal||Ptsl
 +
|-
 +
|3||Spinach-DT||Xbal||Ptsl
 +
|-
 +
|4||Spinach-DT||Xbal||Ptsl
 +
|-
 +
|5||Spinach-DT||Xbal||Ptsl
 +
|-
 +
|6||100bp ladder||--||--
 +
|}
 +
[[File:igku_xxxxxx.xxx]]<br>
 +
{| class="wikitable"
 +
!Lane||Sample||Enzyme1||Enzyme2
 +
|-
 +
|1||100bp ladder||--||--
 +
|-
 +
|2||Pcon-pT181 attenuator||EcoR1||Spel
 +
|-
 +
|3||Pcon-pT181 attenuator||EcoR1||Spel
 +
|-
 +
|4||Pcon-pT181 attenuator||EcoR1||Spel
 +
|-
 +
|5||Pcon-pT181 attenuator||EcoR1||Spel
 +
|-
 +
|6||100bp ladder||--||--
 +
|}
 +
[[File:igku_xxxxxx.xxx]]<br>
 +
{| class="wikitable"
 +
!Lane||Sample||Enzyme1||Enzyme2
 +
|-
 +
|1||Plux-RBS-GFP-DT||EcoR1||Spel
 +
 +
|-
 +
|2||Plux-RBS-GFP-DT ||EcoR1||Spel
 +
|-
 +
|3||Plux-RBS-GFP-DT ||EcoR1||Spel
 +
|-
 +
|4||Plux-RBS-GFP-DT ||EcoR1||Spel
 +
|-
 +
|5||100bp ladder||--||--
 +
|-
 +
|6||apt12-1R-DT||Xbal||Pstl
 +
|-
 +
|7||apt12-1R-DT||Xbal||Pstl
 +
|-
 +
|8||apt12-1R-DT||Xbal||Pstl
 +
|-
 +
|9||apt12-1R-DT||Xbal||Pstl
 +
|}
 +
[[File:igku_xxxxxx.xxx]]<br>
 +
 +
</div>
 +
<!-- ここまでをコピーしてね -->

Revision as of 13:08, 24 September 2013

Contents

Sep 10

Ligation

Nakamoto

stateVectorInserterLigation High ver.2
experiment9/6 Pcon-RBS-luxR-DT1.9µL9/6Plux-RBS-GFP-DT22 µL µL
experiment9/6 Plux2µL9/8 RBS-lysis1-DT6.6µLxx µL
experiment9/6 Plux2µL9/8 RBS-lysis2-DT6.3µLxx µL
experiment9/8 Ptet-PT181 antisense1.6µL9/8 spinach-DT4.4µLxx µL
experiment9/8 Pcon-PT181 attenuator3.4µL9/8 apz 12-1R-PT2.1µLxx µL
experiment9/8 Pcon3.4µL9/8 apz 12-1R-PT2.3µLxx µL
experiment9/8 DT3.0µL9/8 Pcon-PT181 attenuator7.9µLxx µL
experiment9/10 pSB1C31.8µL9/8 F3m2 attenuator4.6µLxx µL
experiment9/10 pSB1C31.8µL9/8 F1 attenuator3.4µLxx µL

Miniprep

Nakamoto

DNAconcentration[µg/mL]260/280260/230
spinach-DT202.41.971.93
Plux-RBS-GFP-DT164.22.802.06
205.41.812.39
RSBJC3-2240.81.731.43
Plaz-1240.81.731.43

Restriction Enzyme Digestion

Nakamoto

9/10　Plux-RBS-GFP-DTEcoR1SpelBufferBSAMilliQtotal
2cuts1µL1µL3µL3µL9.9µL30µL
NC 0µL0µL1µL1µL7.4µL10µL
9/10　Spinach-DTXbalPstlBufferBSAMilliQtotal
2cuts1µL1µL3µL3µL12.1µL30µL
NC 0µL0µL1µL1µL7.5µL10µL
9/6 apt12-1R -DTXbalPstlBufferBSAMilliQtotal
2cuts1µL1µL3µL3µL14.0µL30µL
NC 0µL0µL1µL1µL7.6µL10µL
9/10　Pcon-pt81 attenuator EcoR1SpelBufferBSAMilliQtotal
2cuts1µL1µL3µL3µL12.2µL30µL
NC 0µL0µL1µL1µL7.5µL10µL

Electrophoresis

じっけんしゃ

LaneSampleEnzyme1Enzyme2
1100bp ladder----
2 Plux-RBS-GEP-DTEcoR1Spel
3 Plux-RBS-GEP-DT----
4Spinach-DTXbalPstl
5Spinach-DT----
6aptamer 121R-DTXbalPstl
7aptamer 121R-DT----
8Pcon-pt181 attenuatorEcoR1Spel
9 Pcon-pt181 attenuator----
10100bp ladder----

File:Igku xxxxxx.xxx


Colony PCR

Tatsui

Samplebase pair
9/9 Pcon-pT181 antisense--
9/8 J223100(entA)--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min10sec

Electrophoresis

Tatsui

LaneSample
1100bp ladder
2Pcon Pt181 antisense1+spinach-DT
3Pcon Pt181 antisense2+spinach-DT
4Pcon Pt181 antisense3+spinach-DT
5Pcon Pt181 antisense4+spinach-DT
6Pcon Pt181 antisense5+spinach-DT
7Pcon Pt181 antisense6+spinach-DT
8Pcon Pt181 antisense7+spinach-DT
9Pcon Pt181 antisense8+spinach-DT
10Pcon Pt181 antisense9+spinach-DT
11Pcon Pt181 antisense10+spinach-DT
12Pcon Pt181 antisense11+spinach-DT
13Pcon Pt181 antisense12+spinach-DT
14100bp ladder

File:Igku xxxxxx.xxx

LaneSample
11kbp ladder
2Pcon entA1
3Pcon entA2
4Pcon entA3
51kbp ladder

Gel Extraction

Tatsui

LaneDNAEnzyme
11kbp ladder--
2KaiA(200pg)--
3KaiA(200pg)--
4----
5KaiA(400pg)--
6KaiA(400pg)--
7----
8KaiA(800pg--
9KaiA(800pg)--
101kbp ladder--

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

LaneDNAEnzyme
11kbp ladder--
2PKaiBC(200pg)--
3PKaiBC(200pg)--
4----
5PKaiBC(400pg)--
6PKaiBC(400pg)--
7----
8PKaiBC(800pg--
9PKaiBC(800pg)--
101kbp ladder--

Liquid Culture

Hirano

Samplemedium
9/4 J23100①(JM109)LB(Amp)
9/8 J23100① (entA)LB(Amp)　

Gel Extraction

Tatsui

LaneDNAEnzyme
11kbp ladder--
2KaiC--
3KaiC--
4KaiC--
5----
61kbp ladder--
71kbp ladder--
8KaiC--
9KaiC--
10KaiC-- 11----
121kbp ladder--

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）

Liquid Culture

Tatsui

Samplemedium
9/10 Pcon-pt181 antisense-Spinach-DTPlusgrow 　

Colony PCR

No name

Samplebase pair
9/9 Phad larac-1 RBS-lux1-DT2331
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s2min
Samplebase pair
9/9 Pcon-lacZα (psB4KS)712
9/9 Pcon-Spinach-DT(psB4KS)605
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s42s
Samplebase pair
9/9 Plac+RBS-lysis3}-DT1273
9/9 Pcon+RBS-lux1-DT1079
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min12s

Electrophoresis

No name

LaneSampleEnzyme1Enzyme2
1100bp ladder----
29/9 Pcou-RBS-lacZα-DT(p3B4KS9)-1----
39/9 Pcou-RBS-lacZα-DT(p3B4KS9)-2----
49/9 Pcou-Spinach-DT(pB4KS)-1----
59/9 Pcou-Spinach-DT(pB4KS)-2----
6100bp ladder----

File:Igku xxxxxx.xxx

Transformation

Nakamoto

NameSampleCompetent CellsTotalPlate
F1 attenuator +p3B1C3µLµLµLCP
F3n2 attenuator +p3B1C3µLµLµLCP
F1 antisense +p3B1C3µLµLµLCP
F6 antisense+p3B1C3µLµLµLCP
apt12-P +p3B1C3µLµLµLCP
apt12-1M +p3B1C3µLµLµLCP
Pcon-luxR+Plux-GFPµLµLµLAmp
Plux+RBS-lysis1-DTµLµLµLCP
Plux+RBS-lysis2-DTµLµLµLCP
Ptet-pT181antisense+Spinach-DTµLµLµLCP
Pcon-pT181 attenuator+apt121R-DTµLµLµLAmp
Pcon-1 apt12-1R-DTµLµLµLAmp
DT+Pcon-pT181 attenuatorµLµLµLCP

Electrophoresis

Okazaki

LaneSampleEnzyme1Enzyme2
1100bp ladder----
2Spinach-DTXbalPtsl
3Spinach-DTXbalPtsl
4Spinach-DTXbalPtsl
5Spinach-DTXbalPtsl
6100bp ladder----

File:Igku xxxxxx.xxx

LaneSampleEnzyme1Enzyme2
1100bp ladder----
2Pcon-pT181 attenuatorEcoR1Spel
3Pcon-pT181 attenuatorEcoR1Spel
4Pcon-pT181 attenuatorEcoR1Spel
5Pcon-pT181 attenuatorEcoR1Spel
6100bp ladder----

File:Igku xxxxxx.xxx

LaneSampleEnzyme1Enzyme2
1Plux-RBS-GFP-DTEcoR1Spel
2Plux-RBS-GFP-DT EcoR1Spel
3Plux-RBS-GFP-DT EcoR1Spel
4Plux-RBS-GFP-DT EcoR1Spel
5100bp ladder----
6apt12-1R-DTXbalPstl
7apt12-1R-DTXbalPstl
8apt12-1R-DTXbalPstl
9apt12-1R-DTXbalPstl

File:Igku xxxxxx.xxx

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