Template:Kyoto/Notebook/Sep 18

From 2013.igem.org

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==Sep 18==
==Sep 18==
 +
===Restriction Enzyme Digestion===
 +
<div class="experiment">
 +
<span class="author">No name</span>
 +
{| class="wikitable"
 +
! ||9/18Plux ||Spel||Ptsl||Buffer||BSA||MilliQ||total
 +
|-
 +
|2cuts||1&micro;L||1&micro;L||3&micro;L||3&micro;L||18.8&micro;L|| 30&micro;L
 +
|-
 +
|NC||0&micro;L||0&micro;L||1&micro;L||1&micro;L||6.3&micro;L|| 10&micro;L
 +
|}
 +
{| class="wikitable"
 +
!||9/18Pcon-pT181antisense-spin-DT&#9313;||Xbal||Ptsl||Buffer||BSA||MilliQ||total
 +
|-
 +
|2cuts||1&micro;L||1&micro;L||3&micro;L||3&micro;L||14.5&micro;L|| 30&micro;L
 +
|-
 +
|NC||0&micro;L||0&micro;L||1&micro;L||1&micro;L||6.6&micro;L|| 10&micro;L
 +
|}
 +
{| class="wikitable"
 +
! ||9/16 Plac&#9313;||Spel||Ptsl||Buffer||BSA||MilliQ||total
 +
|-
 +
|2cuts||1&micro;L||1&micro;L||3&micro;L||3&micro;L||14.5&micro;L|| 30&micro;L
 +
|-
 +
|NC||0&micro;L||0&micro;L||1&micro;L||1&micro;L||7.3&micro;L|| 10&micro;L
 +
|}
 +
{| class="wikitable"
 +
! ||9/16 spin-DT||Xbal||Ptsl||Buffer||BSA||MilliQ||total
 +
|-
 +
|2cuts||1&micro;L||1&micro;L||3&micro;L||3&micro;L||4.4&micro;L|| 30&micro;L
 +
|-
 +
|NC||0&micro;L||0&micro;L||1&micro;L||1&micro;L||7.1&micro;L|| 10&micro;L
 +
|}
 +
{| class="wikitable"
 +
! ||9/8 Pcon J23100||Spel||Ptsl||Buffer||BSA||MilliQ||total
 +
|-
 +
|2cuts||1&micro;L||1&micro;L||3&micro;L||3&micro;L||16.3&micro;L|| 30&micro;L
 +
|-
 +
|NC||0&micro;L||0&micro;L||1&micro;L||1&micro;L||7.7&micro;L|| 10&micro;L
 +
|}
 +
{| class="wikitable"
 +
! ||9/16 RBS-lacZ&alpha;-DT||Xbal||Ptsl||Buffer||BSA||MilliQ||total
 +
|-
 +
|2cuts||1&micro;L||1&micro;L||3&micro;L||3&micro;L||13.6&micro;L|| 30&micro;L
 +
|-
 +
|NC||0&micro;L||0&micro;L||1&micro;L||1&micro;L||7.6&micro;L|| 10&micro;L
 +
|}
 +
{| class="wikitable"
 +
! ||9/11 Pcon-pT181antisense-spin-DT&#9312:||Xbal||Ptsl||Buffer||BSA||MilliQ||total
 +
|-
 +
|2cuts||1&micro;L||1&micro;L||3&micro;L||3&micro;L||14.8&micro;L|| 30&micro;L
 +
|-
 +
|NC||0&micro;L||0&micro;L||1&micro;L||1&micro;L||7.6&micro;L|| 10&micro;L
 +
|}
 +
{| class="wikitable"
 +
! ||9/6 apt12-1R-DT||Xbal||Ptsl||Buffer||BSA||MilliQ||total
 +
|-
 +
|2cuts||1&micro;L||1&micro;L||3&micro;L||3&micro;L||14.1&micro;L|| 30&micro;L
 +
|-
 +
|NC||0&micro;L||0&micro;L||1&micro;L||1&micro;L||7.6&micro;L|| 10&micro;L
 +
|}
 +
{| class="wikitable"
 +
! ||9/3 pT181antisense||Xbal||Ptsl||Buffer||BSA||MilliQ||total
 +
|-
 +
|2cuts||1&micro;L||1&micro;L||3&micro;L||3&micro;L||15.7&micro;L|| 30&micro;L
 +
|-
 +
|NC||0&micro;L||0&micro;L||1&micro;L||1&micro;L||7.7&micro;L|| 10&micro;L
 +
|}
 +
{| class="wikitable"
 +
! ||9/14 pSB4K5||EcoR1||Spel||Buffer||BSA||MilliQ||total
 +
|-
 +
|2cuts||1&micro;L||1&micro;L||3&micro;L||3&micro;L||14.7&micro;L|| 30&micro;L
 +
|-
 +
|NC||0&micro;L||0&micro;L||1&micro;L||1&micro;L||7.6&micro;L|| 10&micro;L
 +
|}
 +
{| class="wikitable"
 +
! ||8/31 Plux-RBS-GFP-DT ||EcoR1||Spel||Buffer||BSA||MilliQ||total
 +
|-
 +
|2cuts||1&micro;L||1&micro;L||3&micro;L||3&micro;L||12.8&micro;L|| 30&micro;L
 +
|-
 +
|NC||0&micro;L||0&micro;L||1&micro;L||1&micro;L||7.5&micro;L|| 10&micro;L
 +
|}
 +
{| class="wikitable"
 +
! ||9/12 RBS-lysis3-DT ||Xbal||Ptsl||Buffer||BSA||MilliQ||total
 +
|-
 +
|2cuts||1&micro;L||1&micro;L||3&micro;L||3&micro;L||16.8&micro;L|| 30&micro;L
 +
|-
 +
|NC||0&micro;L||0&micro;L||1&micro;L||1&micro;L||7.6&micro;L|| 10&micro;L
 +
|}
 +
{| class="wikitable"
 +
! ||9/18 Pcon-pT181attenuator||EcoR1||Spel||Buffer||BSA||MilliQ||total
 +
|-
 +
|2cuts||1&micro;L||1&micro;L||3&micro;L||3&micro;L||7.7&micro;L|| 30&micro;L
 +
|-
 +
|NC||0&micro;L||0&micro;L||1&micro;L||1&micro;L||7.3&micro;L|| 10&micro;L
 +
|}
 +
</div>
 +
 +
===Electrophoresis===
 +
 +
<div class="experiment">
 +
<span class="author">No name</span>
 +
{| class="wikitable"
 +
!Lane||Sample||Enzyme1||Enzyme2
 +
|-
 +
|1||Plae&#9313; ||Spel||Ptsl
 +
|-
 +
|2||RBS-lysis3-DT||Xbal||Ptsl
 +
|-
 +
|3||Plux-RBS-GFP-DT||EcoR1||Spel
 +
|-
 +
|4||PSB4K5||EcoR1||Spel
 +
|-
 +
|5||Pcon||Spel||Pstl
 +
|-
 +
|6||Plux||Spel||Pstl
 +
|-
 +
|7||1kbp ladder||--||--
 +
|-
 +
|8||Plae&#9313; ||--||--
 +
|-
 +
|9||RBS-lysis3-DT||--||--
 +
|-
 +
|10||Plux-RBS-GFP-DT||--||--
 +
|-
 +
|11||PSB4K5||--||--
 +
|-
 +
|12||Pcon||--||--
 +
|-
 +
|13||Plux||--||--
 +
|}
 +
[[File:igku_xxxxxx.xxx]]<br>
 +
</div>
 +
 +
===Electrophoresis===
 +
 +
<div class="experiment">
 +
<span class="author">Okazaki</span>
 +
{| class="wikitable"
 +
!Lane||Sample||Enzyme1||Enzyme2
 +
|-
 +
|1||100bp ladder ||-- ||--
 +
|-
 +
|2||Pcon pT181antisense spinach-DT&#9313;||Xbal||Pstl
 +
|-
 +
|3||Spinach-DT||Xbal||Pstl
 +
|-
 +
|4||RBS-lacZ&alpha;-DT|| Xbal||Pstl
 +
|-
 +
|5||Pcon pT181antisense-Spinach-DT&#9312;|| Xbal||Pstl
 +
|-
 +
|6||apt12-1R-DT||Xbal||Pstl
 +
|-
 +
|7||pT181attenuator||Xbal||Pstl
 +
|-
 +
|8||Pcon attenuator||EcoR1||Spel
 +
|}
 +
{| class="wikitable"
 +
!Lane||Sample||Enzyme1||Enzyme2
 +
|-
 +
|1||100bp ladder ||-- ||--
 +
|-
 +
|2||Pcon pT181antisense spinach-DT&#9313;||Xbal||Pstl
 +
|-
 +
|3||Spinach-DT||Xbal||Pstl
 +
|-
 +
|4||RBS-lacZ&alpha;-DT|| Xbal||Pstl
 +
|-
 +
|5||Pcon pT181antisense-Spinach-DT&#9312;|| Xbal||Pstl
 +
|-
 +
|6||apt12-1R-DT||Xbal||Pstl
 +
|-
 +
|7||pT181attenuator||Xbal||Pstl
 +
|-
 +
|8||Pcon attenuator||EcoR1||Spel
 +
|}
 +
{| class="wikitable"
 +
!Lane||Sample||Enzyme1||Enzyme2
 +
|-
 +
|1||100bp ladder ||-- ||--
 +
|-
 +
|2||Pcon pT181antisense spinach-DT&#9313;||--||--
 +
|-
 +
|3||Spinach-DT||--||--
 +
|-
 +
|4||RBS-lacZ&alpha;-DT||--||--
 +
|-
 +
|5||Pcon pT181antisense-Spinach-DT&#9312;||--||--
 +
|-
 +
|6||apt12-1R-DT||--||--
 +
|-
 +
|7||pT181attenuator||--||--
 +
|-
 +
|8||Pcon attenuator||--||--
 +
|}
 +
[[File:igku_xxxxxx.xxx]]<br>
 +
</div>
 +
 +
===Gel Extraction===
 +
<div class="experiment">
 +
<span class="author">No name</span>
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||1kbp ldder||--
 +
|-
 +
|2||Plac&#9313;||Spel+Pstl
 +
|-
 +
|3||Plac&#9313;||Spel+Pstl
 +
|
 +
|4||--||--
 +
|-
 +
|5||RBS-lysis3-DT||Xbal+Pstl
 +
|-
 +
|6||RBS-lysis3-DT||Xbal+Pstl
 +
|-
 +
|7||--||--
 +
|-
 +
|8||Plux-RBS-GFP-DT||EcoR1+Spel
 +
|-
 +
|9||Plux-RBS-GFP-DT||EcoR1+Spel
 +
|-
 +
|10||--||--
 +
|-
 +
|11|PSB4KS||EcoR1+Spel
 +
|-
 +
|12|PSB4KS||EcoR1+Spel
 +
|}
 +
[[File:igku_xxbeforexx.xxx]]<br>
 +
[[File:igku_xxafterxx.xxx]]<br>
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||1kbp ldder||--
 +
|-
 +
|2||Pcon||Spel+Pstl
 +
|-
 +
|3|| Pcon||Spel+Pstl
 +
|
 +
|4||--||--
 +
|-
 +
|5||Plux||Spel+PStl
 +
|-
 +
|6|| Plux||Spel+PStl
 +
|}
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||Pcon PT181 antisense spinach-DT||Xbal+Pstl
 +
|-
 +
|2|| Pcon PT181 antisense Spinach-DT||Xbal+Pstl
 +
|-
 +
|3||100bp ladder ||--
 +
|
 +
|4||Pcon PT181 antisense Spinach-DT||Xbal+Pstl
 +
|-
 +
|5|| Pcon PT181 antisense Spinach-DT||Xbal+Pstl
 +
|-
 +
|6|| 1kbp ladder||--
 +
|}
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||100bp ladder ||--
 +
|-
 +
|2||apt12-1R-DT||Xbal+Pstl
 +
|-
 +
|3||apt12-1R-DT||Xbal+Pstl
 +
|
 +
|4||--||--
 +
|-
 +
|5||PT181 attenuator||Xbal+Pstl
 +
|-
 +
|6||PT181 attenuator||Xbal+Pstl
 +
|}
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||100bp ladder ||--
 +
|-
 +
|2||Pcoa attenuator||EcoR1+Pstl
 +
|-
 +
|3||Pcoa attenuator||EcoR1+Pstl
 +
|
 +
|4||--||--
 +
|-
 +
|5||RBS-lacZ&alpha;-DT||Xbal+Pstl
 +
|-
 +
|6||RBS-lacZ&alpha;-DT ||Xbal+Pstl
 +
|}</div>

Revision as of 14:28, 24 September 2013

Contents

Sep 18

Restriction Enzyme Digestion

No name

9/18Plux SpelPtslBufferBSAMilliQtotal
2cuts1µL1µL3µL3µL18.8µL 30µL
NC0µL0µL1µL1µL6.3µL 10µL
9/18Pcon-pT181antisense-spin-DT②XbalPtslBufferBSAMilliQtotal
2cuts1µL1µL3µL3µL14.5µL 30µL
NC0µL0µL1µL1µL6.6µL 10µL
9/16 Plac②SpelPtslBufferBSAMilliQtotal
2cuts1µL1µL3µL3µL14.5µL 30µL
NC0µL0µL1µL1µL7.3µL 10µL
9/16 spin-DTXbalPtslBufferBSAMilliQtotal
2cuts1µL1µL3µL3µL4.4µL 30µL
NC0µL0µL1µL1µL7.1µL 10µL
9/8 Pcon J23100SpelPtslBufferBSAMilliQtotal
2cuts1µL1µL3µL3µL16.3µL 30µL
NC0µL0µL1µL1µL7.7µL 10µL
9/16 RBS-lacZα-DTXbalPtslBufferBSAMilliQtotal
2cuts1µL1µL3µL3µL13.6µL 30µL
NC0µL0µL1µL1µL7.6µL 10µL
9/11 Pcon-pT181antisense-spin-DT&#9312:XbalPtslBufferBSAMilliQtotal
2cuts1µL1µL3µL3µL14.8µL 30µL
NC0µL0µL1µL1µL7.6µL 10µL
9/6 apt12-1R-DTXbalPtslBufferBSAMilliQtotal
2cuts1µL1µL3µL3µL14.1µL 30µL
NC0µL0µL1µL1µL7.6µL 10µL
9/3 pT181antisenseXbalPtslBufferBSAMilliQtotal
2cuts1µL1µL3µL3µL15.7µL 30µL
NC0µL0µL1µL1µL7.7µL 10µL
9/14 pSB4K5EcoR1SpelBufferBSAMilliQtotal
2cuts1µL1µL3µL3µL14.7µL 30µL
NC0µL0µL1µL1µL7.6µL 10µL
8/31 Plux-RBS-GFP-DT EcoR1SpelBufferBSAMilliQtotal
2cuts1µL1µL3µL3µL12.8µL 30µL
NC0µL0µL1µL1µL7.5µL 10µL
9/12 RBS-lysis3-DT XbalPtslBufferBSAMilliQtotal
2cuts1µL1µL3µL3µL16.8µL 30µL
NC0µL0µL1µL1µL7.6µL 10µL
9/18 Pcon-pT181attenuatorEcoR1SpelBufferBSAMilliQtotal
2cuts1µL1µL3µL3µL7.7µL 30µL
NC0µL0µL1µL1µL7.3µL 10µL

Electrophoresis

No name

LaneSampleEnzyme1Enzyme2
1Plae② SpelPtsl
2RBS-lysis3-DTXbalPtsl
3Plux-RBS-GFP-DTEcoR1Spel
4PSB4K5EcoR1Spel
5PconSpelPstl
6PluxSpelPstl
71kbp ladder----
8Plae② ----
9RBS-lysis3-DT----
10Plux-RBS-GFP-DT----
11PSB4K5----
12Pcon----
13Plux----

File:Igku xxxxxx.xxx

Electrophoresis

Okazaki

LaneSampleEnzyme1Enzyme2
1100bp ladder -- --
2Pcon pT181antisense spinach-DT②XbalPstl
3Spinach-DTXbalPstl
4RBS-lacZα-DT XbalPstl
5Pcon pT181antisense-Spinach-DT① XbalPstl
6apt12-1R-DTXbalPstl
7pT181attenuatorXbalPstl
8Pcon attenuatorEcoR1Spel
LaneSampleEnzyme1Enzyme2
1100bp ladder -- --
2Pcon pT181antisense spinach-DT②XbalPstl
3Spinach-DTXbalPstl
4RBS-lacZα-DT XbalPstl
5Pcon pT181antisense-Spinach-DT① XbalPstl
6apt12-1R-DTXbalPstl
7pT181attenuatorXbalPstl
8Pcon attenuatorEcoR1Spel
LaneSampleEnzyme1Enzyme2
1100bp ladder -- --
2Pcon pT181antisense spinach-DT②----
3Spinach-DT----
4RBS-lacZα-DT----
5Pcon pT181antisense-Spinach-DT①----
6apt12-1R-DT----
7pT181attenuator----
8Pcon attenuator---- 
File:Igku xxxxxx.xxx

Gel Extraction

No name

LaneDNAEnzyme
11kbp ldder--
2Plac②Spel+Pstl
3Plac②Spel+Pstl 4----
5RBS-lysis3-DTXbal+Pstl
6RBS-lysis3-DTXbal+Pstl
7----
8Plux-RBS-GFP-DTEcoR1+Spel
9Plux-RBS-GFP-DTEcoR1+Spel
10----
PSB4KSEcoR1+Spel
PSB4KSEcoR1+Spel

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

LaneDNAEnzyme
11kbp ldder--
2PconSpel+Pstl
3 PconSpel+Pstl 4----
5PluxSpel+PStl
6 PluxSpel+PStl
LaneDNAEnzyme
1Pcon PT181 antisense spinach-DTXbal+Pstl
2 Pcon PT181 antisense Spinach-DTXbal+Pstl
3100bp ladder -- 4Pcon PT181 antisense Spinach-DTXbal+Pstl
5 Pcon PT181 antisense Spinach-DTXbal+Pstl
6 1kbp ladder--
LaneDNAEnzyme
1100bp ladder --
2apt12-1R-DTXbal+Pstl
3apt12-1R-DTXbal+Pstl 4----
5PT181 attenuatorXbal+Pstl
6PT181 attenuatorXbal+Pstl
LaneDNAEnzyme
1100bp ladder --
2Pcoa attenuatorEcoR1+Pstl
3Pcoa attenuatorEcoR1+Pstl 4----
5RBS-lacZα-DTXbal+Pstl
6RBS-lacZα-DT Xbal+Pstl
Retrieved from "http://2013.igem.org/Template:Kyoto/Notebook/Sep_18"