Template:Kyoto/Notebook/Sep 15

From 2013.igem.org

(Difference between revisions)
(Restriction Enzyme Digestion)
(Sep 15)
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</div>
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===Electrophoresis===
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<div class="experiment">
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<span class="author">No Name</span>
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{| class="wikitable"
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!Lane||Sample||Enzyme1||Enzyme2
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|-
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|1,14 || || ||
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|-
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|2 ||Pcon-RBS-tetR-DT ||EcoRI ||SpeI
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|-
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|3 ||Pcon-RBS-tetR-DT || ||
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|-
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|4 ||Pcon-pT181 attenuator-DT ||EcoRI ||SpeI
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|-
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|5 ||Pcon-pT181 attenuator-DT || ||
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|-
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|6 ||Pcon-apt12-1R-DT ||EcoRI ||SpeI
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|-
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|7 ||Pcon-apt12-1R-DT || ||
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|-
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|8 ||Pcon-spin-DT ||EcoRI ||SpeI
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|-
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|9 ||Pcon-spin-DT || ||
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|-
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|10 ||Pcon-pT181 antisense-spin-DT ||XbaI ||PstI
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|-
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|11 ||Pcon-pT181 antisense-spin-DT || ||
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|-
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|12 ||RBS-lacZ&alpha;-DT ||XbaI ||PstI
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|-
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|13 ||RBS-lacZ&alpha;-DT || || 
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|-
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|15 ||Ptet-RBS-lacZ&alpha;-DT ||EcoRI ||XbaI
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|-
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|16 ||Ptet-RBS-lacZ&alpha;-DT || ||
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|-
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|17 ||1kbp ladder || ||
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|}
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[[File:igku_xxxxxx.xxx]]<br>
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Revision as of 14:38, 24 September 2013

Contents

Sep 15

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
Pλ-luxI225 1.59 0.83

Liquid Culture

No name

Samplemedium
9/13 Pλ-luxI-1
9/7 pSB4K5-1
9/11 Plac-3

Ligation

じっけんしゃ

stateVectorInserterLigation High ver.2
experiment9/13 Pcon(SpeI & PstI)3.6 µL9/12 RBS-lacZα-DT(XbaI & PstI)4.7 µL8 µL

Restriction Enzyme Digestion

No Name

DNAEcorISpeIBufferBSAMilliQtotal
9/7Pcon-RBS-tetR-DT (EcoRI & SpeI)15 µL1 µL1 µL3 µL3 µL6 µL30 µL
NC0.7 µL0 µL0 µL1 µL1 µL7.3 µL10 µL


DNAEcoRISpeIBufferBSAMilliQtotal
9/12 Pcon-pT181 attenuator-DT (EcoRI & SpeI)6.8 µL1 µL1 µL3 µL3 µL15.2 µL30 µL
NC0.3 µL0 µL0 µL1 µL1 µL7.7 µL10 µL


DNAEcoRISpeIBufferBSAMilliQtotal
9/12 Pcon-apt12_1R-DT(EcoRI & SpeI)6.6 µL1 µL1 µL3 µL3 µL15.4 µL30 µL
NC0.3 µL0 µL0 µL1 µL1 µL7.7 µL10 µL


DNAEcoRISpeIBufferBSAMilliQtotal
9/8 Pcon-spin-DT (EcoRI & SpeI)7.6 µL1 µL1 µL3 µL3 µL15.4 µL30 µL
NC0.3 µL0 µL0 µL1 µL1 µL7.7 µL10 µL


DNAXbaIPstIBufferBSAMilliQtotal
9/11 Pcon-pT181 anticodone-spin-DT(XbaI & PstI)7.2 µL1 µL1 µL3 µL3 µL14.8 µL30 µL
NC0.4 µL0 µL0 µL1 µL1 µL7.6 µL10 µL


DNAXbaIPstIBufferBSAMilliQtotal
8/17 RBS-lacZα-DT (XbaI & PstI)5.3 µL1 µL1 µL3 µL3 µL16.7 µL30 µL
NC0.5 µL0 µL0 µL1 µL1 µL7.5 µL10 µL


DNAEcoRIXbaIBufferBSAMilliQtotal
9/7 Ptet-RBS-LacZα-DT (EcoRI & XbaI)2.9 µL1 µL1 µL3 µL3 µL18.5 µL30 µL
NC1.5 µL0 µL0 µL1 µL1 µL7 µL10 µL


Electrophoresis

No Name

LaneSampleEnzyme1Enzyme2
1,14
2 Pcon-RBS-tetR-DT EcoRI SpeI
3 Pcon-RBS-tetR-DT
4 Pcon-pT181 attenuator-DT EcoRI SpeI
5 Pcon-pT181 attenuator-DT
6 Pcon-apt12-1R-DT EcoRI SpeI
7 Pcon-apt12-1R-DT
8 Pcon-spin-DT EcoRI SpeI
9 Pcon-spin-DT
10 Pcon-pT181 antisense-spin-DT XbaI PstI
11 Pcon-pT181 antisense-spin-DT
12 RBS-lacZα-DT XbaI PstI
13 RBS-lacZα-DT
15 Ptet-RBS-lacZα-DT EcoRI XbaI
16 Ptet-RBS-lacZα-DT
17 1kbp ladder

File:Igku xxxxxx.xxx