Team:Buenos Aires/ model
From 2013.igem.org
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Our objective was to assemble a system that could respond to different concentrations of a specific contaminant in water. | Our objective was to assemble a system that could respond to different concentrations of a specific contaminant in water. | ||
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More specifically, the idea was to produce different amounts of a coloured pigment depending on the concentration of the contaminant present in water. | More specifically, the idea was to produce different amounts of a coloured pigment depending on the concentration of the contaminant present in water. | ||
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+ | As mention all over this wiki, we worked with Arsenic knowing that an Ars operon exist in nature and more important, it exists as a biobrick in IGEM. | ||
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As it is shown in the figure below, in the presence of arsenite (1000 ppb) a typical transcriptional induction is observed over time. | As it is shown in the figure below, in the presence of arsenite (1000 ppb) a typical transcriptional induction is observed over time. | ||
There is a lag of 3 hours in the mRFP produciton after adding arsenite. | There is a lag of 3 hours in the mRFP produciton after adding arsenite. | ||
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== '''mRFP stability over time''' == | == '''mRFP stability over time''' == | ||
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Revision as of 20:49, 24 September 2013
Deterministic Model
Introduction
Our objective was to assemble a system that could respond to different concentrations of a specific contaminant in water.
More specifically, the idea was to produce different amounts of a coloured pigment depending on the concentration of the contaminant present in water.
As mention all over this wiki, we worked with Arsenic knowing that an Ars operon exist in nature and more important, it exists as a biobrick in IGEM.
Results
As it is shown in the figure below, in the presence of arsenite (1000 ppb) a typical transcriptional induction is observed over time. There is a lag of 3 hours in the mRFP produciton after adding arsenite.